A5101102358- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Vector-borne infectious diseases
- Toxin Mechanisms and Immunotoxins
- Antimicrobial Peptides and Activities
- Viral Infections and Vectors
- Antibiotics Pharmacokinetics and Efficacy
- Plant Virus Research Studies
- Advances in Cucurbitaceae Research
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- RNA and protein synthesis mechanisms
- Insect symbiosis and bacterial influences
- Autophagy in Disease and Therapy
- Insect and Pesticide Research
- Garlic and Onion Studies
- Cystic Fibrosis Research Advances
- Salmonella and Campylobacter epidemiology
- Rabies epidemiology and control
- Plant tissue culture and regeneration
- Bacterial Identification and Susceptibility Testing
- Transgenic Plants and Applications
- CRISPR and Genetic Engineering
Nankai University
2016-2025
Liaoning University
2025
Northwest A&F University
2016-2024
State Key Laboratory of Medicinal Chemical Biology
2015-2024
Lingnan Normal University
2021-2024
Shanghai University of Medicine and Health Sciences
2023-2024
Hubei University of Technology
2020-2022
Northwest Institute of Mechanical and Electrical Engineering
2022
The Ohio State University
2007-2017
Hubei University
2017
Abstract Salmonella Typhimurium establishes systemic infection by replicating in host macrophages. Here we show that macrophages infected with S . exhibit upregulated glycolysis and decreased serine synthesis, leading to accumulation of glycolytic intermediates. The effects on synthesis are mediated bacterial protein SopE2, a type III secretion system (T3SS) effector encoded pathogenicity island SPI-1. changes metabolism promote intracellular replication via two mechanisms: glucose levels...
Ehrlichia chaffeensis is an obligatory intracellular bacterium that causes a potentially fatal emerging zoonosis, human monocytic ehrlichiosis. E. has limited capacity for biosynthesis and metabolism thus depends mostly on host-synthesized nutrients growth. Although the host cell cytoplasm rich with these nutrients, as confined within early endosome-like membrane-bound compartment, only enter compartment can be used by this bacterium. How occurs unknown. We found ehrlichial replication...
Bacterial persister cells are dormant and highly tolerant to lethal antibiotics, which believed be the major cause of recurring chronic infections. Activation toxins bacterial toxin-antitoxin systems inhibits growth plays an important role in formation. However, little is known about overall gene expression profile upon toxin activation. More importantly, how persisters evade host immune clearance remains poorly understood. Here we demonstrate that a Pseudomonas aeruginosa system HigB-HigA...
Abstract The rising antibiotic resistance of bacteria imposes a severe threat on human health. Inhibition bacterial virulence is an alternative approach to develop new antimicrobials. Molecules targeting resistant enzymes have been used in combination with cognate antibiotics. It might be ideal that molecule can simultaneously suppress factors and resistance. Here we combined genetic computer-aided inhibitor screening search for such molecules against the pathogen Pseudomonas aeruginosa . To...
Coronavirus disease 2019(COVID-19) caused a large number of infections worldwide. Although some patients recovered from the disease, other problems that accompanied it, such as cardiac injury, could affect patient's subsequent quality life and prognosis.To clarify molecular mechanism injury in SARS-CoV-2 Infection.The RNA-Seq dataset (GSE184715) comparing expression profiling Mock human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) SARS-CoV-2-infected hiPSC-CMs was...
ABSTRACT Murepavadin is a peptidomimetic that specifically targets the lipopolysaccharide transport protein LptD of Pseudomonas aeruginosa . Here, we found murepavadin enhances bactericidal efficacies tobramycin and amikacin. We further demonstrated bacterial respiration activity subsequent membrane potential, which promotes intracellular uptake aminoglycoside antibiotics. In addition, murepavadin-amikacin combination displayed synergistic effect in murine pneumonia model.
Environmental metabolites and metabolic pathways significantly influence bacterial pathogenesis interspecies competition. We previously discovered that a mutation in the triosephosphate isomerase gene, tpiA , Pseudomonas aeruginosa led to defective type III secretion increased susceptibility aminoglycoside antibiotics. In this study, we found enhances Las quorum sensing system due reduced translation of negative regulator RsaL. Further investigations demonstrated an upregulation CspC, CspA...
ABSTRACT The type IV secretion (T4S) system is critical for the virulence of several pathogens. In rickettsial pathogen Ehrlichia chaffeensis , virBD genes are split into two operons, virB3 - virB6 (preceded by sodB ) and virB8 virD4 operons. Between these there duplications virB4 virB9 . this study we found that transcription all five loci was downregulated prior to release E. from host THP-1 cells upregulated at initiation exponential growth. Electrophoretic mobility shift assays revealed...
Summary Ehrlichia chaffeensis is an obligate intracellular bacterium that causes human monocytic ehrlichiosis. Ehrlichiae have a biphasic developmental cycle consisting of dense‐cored cells (DCs) and reticulate (RCs). Isolated DCs are more stress resistant infectious than RCs. Here, we report response regulator, CtrA was upregulated in monocytes at the late growth stage when develop. E. bound to promoters late‐stage transcribed genes: ctrA, ompA (peptidoglycan‐associated lipoprotein), bolA...
Carbon metabolism plays an essential role in bacterial pathogenesis and susceptibility to antibiotics. In Pseudomonas aeruginosa, Crc, Hfq, a small RNA, CrcZ, are central regulators of carbon metabolism. By screening mutants genes involved metabolism, we found that mutation the tpiA gene reduces expression type III secretion system (T3SS) resistance aminoglycoside TpiA is triosephosphate isomerase reversibly converts glyceraldehyde 3-phosphate dihydroxyacetone phosphate, key step connecting...
Polymyxin B and E (colistin) are the last resorts to treat multidrug-resistant Gram-negative pathogens. Pseudomonas aeruginosa is intrinsically resistant a variety of antibiotics. The PhoP-PhoQ two-component regulatory system contributes resistance polymyxins by regulating an arnBCADTEF-pmrE operon that encodes lipopolysaccharide modification enzymes. To identify additional PhoP-regulated genes contribute tolerance polymyxin B, we performed chromatin immunoprecipitation sequencing (ChIP-Seq)...
The two-component system (TCS) composed of a pair sensor histidine kinase and response regulator, allows bacteria to sense signals respond changes in their environment through specific gene activation or repression. present study examined TCS the obligatory intracellular Ehrlichia chaffeensis Anaplasma phagocytophilum, that cause human monocytic ehrlichiosis (HME) granulocytic anaplasmosis (HGA) respectively. genomes E. A. phagocytophilum were each predicted encode three pairs TCSs. All six...
Ehrlichia chaffeensis, the etiologic agent of human monocytic ehrlichiosis, replicates in early endosomes by avoiding lysosomal fusion monocytes and macrophages. In E. chaffeensis we predicted three pairs putative two-component regulatory systems (TCSs) designated PleC-PleD, NtrY-NtrX, CckA-CtrA based on amino acid sequence homology. present study to determine biochemical specificities TCSs, recombinant proteins histidine kinase (HK) domains (rPleCHKD, rNtrYHKD, MBP-rCckAHKD) full-length...
How the obligatory intracellular bacterium Ehrlichia chaffeensis begins to replicate upon entry into human monocytes is poorly understood. Here, we examined potential role of amino acids in initiating replication. PutA converts proline glutamate, and GlnA glutamate glutamine. E. complemented Escherichia coli putA glnA mutants. Methionine sulfoximine, a glutamine synthetase inhibitor, inhibited activity infection cells. Incubation with cells rapidly induced expression that peaked at 24 h...
Pseudomonas aeruginosa is a Gram negative opportunistic pathogenic bacterium, which causes acute and chronic infections. Upon entering host, bacteria alter global gene expression to adapt host environment avoid clearance by host. Enolase glycolytic enzyme involved in carbon metabolism. It also component of RNA degradosome, processing regulation. Here we report that enolase required for the pathogenesis P. murine pneumonia model. Mutation coding (eno) increased bacterial susceptibility...
Post-transcriptional regulation enables bacteria to quickly response environmental stresses. Polynucleotide phosphorylase (PNPase), which contains an N-terminal catalytic core and C-terminal RNA binding KH-S1 domains, is involved in processing. Here we demonstrate that Pseudomonas aeruginosa the domains of PNPase are required for type III secretion system (T3SS) bacterial virulence. Transcriptome analysis revealed a pleiotropic role gene regulation. Particularly, level exsA was decreased...
During infection of a host, Pseudomonas aeruginosa orchestrates global gene expression to adapt the host environment and counter immune attacks. P. harbours hundreds regulatory genes that play essential roles in controlling expression. However, their contributions bacterial pathogenesis remain largely unknown. In this study, we analysed transcriptomic profile cells isolated from lungs infected mice examined upregulated virulence. Mutation novel pvrA (PA2957) attenuated virulence an acute...
Posttranscriptional regulation plays an essential role in the quick adaptation of pathogenic bacteria to host environments, and RNases play key roles this process by modifying small RNAs mRNAs. We find that Pseudomonas aeruginosa endonuclease YbeY is required for rRNA processing bacterial virulence a murine acute pneumonia model. Transcriptomic analyses reveal knocking out ybeY gene results downregulation oxidative stress response genes, including catalase genes katA katB Consistently,...
The human blood-brain barrier (BBB) comprises a single layer of brain microvascular endothelial cells (HBMECs) protecting the from bloodborne pathogens. Meningitis is among most serious diseases, but mechanisms by which major meningitis-causing bacterial pathogens cross BBB to reach remain poorly understood. We found that
Differential gene expression is a key strategy adopted by the Lyme disease spirochaete, Borrelia burgdorferi, for adaptation and survival in mammalian host tick vector. Many B. burgdorferi surface lipoproteins fall into two distinct groups according to their patterns: one group primarily expressed other mammal. Here, we show that Fur homologue this bacterium, also known as oxidative stress regulator (BosR), required repression of outer protein A (OspA) OspD Furthermore, BosR binds directly...
Infections by Pseudomonas aeruginosa are often difficult to cure due its high intrinsic and acquired antibiotic resistance. Once colonized in the human hosts, P. acquires genetic mutations which render bacteria resistance as well ability better adapt host environment. Deciphering evolutionary traits may provide important insights into development of effective combinatory therapy treat infections. In this study, we investigated molecular mechanisms a clinical isolate (ISP50) yields an...
The evolution of bacterial antibiotic resistance is exhausting the list currently used antibiotics and endangers those in pipeline. combination a promising strategy that may suppress development and/or achieve synergistic therapeutic effects. Eravacycline newly approved effective against variety multidrug-resistant (MDR) pathogens. However, to eravacycline strategies remain unexplored. Here, we demonstrated carbapenem-resistant Klebsiella pneumoniae clinical isolate quickly developed...
Abstract Pseudomonas aeruginosa is capable of causing acute and chronic infections in various host tissues, which depends on its abilities to effectively utilize host-derived nutrients produce protein virulence factors toxic compounds. However, the regulatory mechanisms that direct metabolic intermediates towards production compounds are poorly understood. We previously identified a PvrA controls genes involved fatty acid catabolism by binding palmitoyl-coenzyme A (CoA). In this study,...
Toxin-antitoxin (TA) systems play important roles in bacteria persister formation. Increasing evidence demonstrate the of TA regulating virulence factors pathogenic bacteria. The toxin HigB Pseudomonas aeruginosa contributes to formation and regulates expression multiple biofilm However, regulatory mechanism remains elusive. In this study, we explored mediated pathways. We that decreases intracellular level c-di-GMP, which is responsible for increased type III secretion system (T3SS) genes...