A5115604803- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Cell Image Analysis Techniques
- Gene expression and cancer classification
- Acute Myeloid Leukemia Research
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Acute Lymphoblastic Leukemia research
- Endodontics and Root Canal Treatments
- Single-cell and spatial transcriptomics
- Dental materials and restorations
- Probability and Risk Models
- Financial Risk and Volatility Modeling
- RNA and protein synthesis mechanisms
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- RNA Research and Splicing
- Insurance, Mortality, Demography, Risk Management
- Advanced biosensing and bioanalysis techniques
- Renal and related cancers
- Polysaccharides and Plant Cell Walls
- Genetics and Neurodevelopmental Disorders
- Metabolism and Genetic Disorders
- Antioxidant Activity and Oxidative Stress
- TGF-β signaling in diseases
- Marine animal studies overview
Northwestern Polytechnical University
2024-2025
East China University of Science and Technology
2024-2025
Huazhong University of Science and Technology
2021-2024
Shanghai East Hospital
2024
University of Nottingham
2024
Children's Medical Center
2017-2023
The University of Texas Southwestern Medical Center
2017-2023
Communication University of China
2022
Union Hospital
2021
Central China Normal University
2021
Abstract Tissue-specific gene expression requires coordinated control of gene-proximal and -distal cis -regulatory elements (CREs), yet functional analysis gene-distal CREs such as enhancers remains challenging. Here we describe CRISPR/dCas9-based enhancer-targeting epigenetic editing systems, enCRISPRa enCRISPRi, for efficient enhancer function in situ vivo. Using dual effectors capable re-writing enhancer-associated chromatin modifications, show that enCRISPRi modulate transcription by...
Abstract Epigenetic gene regulation and metabolism are highly intertwined, yet little is known about whether altered epigenetics influence cellular during cancer progression. Here, we show that EZH2 NRASG12D mutations cooperatively induce progression of myeloproliferative neoplasms to penetrant, transplantable, lethal myeloid leukemias in mice. EZH1, an homolog, indispensable for EZH2-deficient leukemia-initiating cells constitutes epigenetic vulnerability. BCAT1, which catalyzes the...
Abstract Background The halophilic bacterium Halomonas elongata is an industrially important strain for ectoine production, with high value and intense research focus. While existing studies primarily delve into the adaptive mechanisms of this under fixed salt concentrations, there a notable dearth attention regarding its response to fluctuating saline environments. Consequently, stress H. shock remains inadequately understood. Results This study investigated mechanism when exposed NaCl at...
Mutations in protein-coding genes are well established as the basis for human cancer, yet how alterations within noncoding genome, a substantial fraction of which contain cis-regulatory elements (CRE), contribute to cancer pathophysiology remains elusive. Here, we developed an integrative approach systematically identify and characterize regulatory variants with functional consequences hematopoietic malignancies. Combining targeted resequencing lineage-associated CREs mutation discovery,...
Lentinan (LNT), a typical triple helix β-glucan extracted from Lentinus edodes, has been widely used as functional food and an orally administered drug. However, its oral pharmacokinetics rarely reported. The aim of this work is to systematically study the intestinal absorption mechanism LNT after administration. Radioactive 99m-technetium (99mTc) was introduced label determine plasma concentration, tissue distribution, excretion in rats results confirmed LNT, with maximal concentration...
Prunus mume (Armeniaca Sieb. var. f. viridicalyx (Makino) T. Y. Chen) is a common landscape plant. It has rich flavonoid content, which shows strong biological activities. In this study, the application potential of flavonoids in anti-photoaging cosmetics was developed. Firstly, were extracted by heating reflux method, and total content reached 192 mg/g under optimal process conditions determined orthogonal testing. Then, crude extract (PMCE) purified NKA-II resin column chromatography,...
Uropathogenic
Abstract Small nucleolar RNA host gene 6 (SNHG6) is a newly discovered long non-coding (lncRNA), while the regulatory mechanism of SNHG6 in chondrosarcoma largely unknown. Here we found that expression was upregulated and showed positive correlation with progression chondrosarcoma. Functional assays demonstrated required for proliferation, migration, invasion cells. Mechanistic study revealed could recruit EZH2 maintain high level H3K27me3 to repress transcription tumor-suppressor genes,...
Abstract Cancers develop from the accumulation of somatic mutations, yet it remains unclear how oncogenic lesions cooperate to drive cancer progression. Using a mouse model harboring NRas G12D and EZH2 mutations that recapitulates leukemic progression, we employ single-cell transcriptomic profiling map cellular composition gene expression alterations in healthy or diseased bone marrows during leukemogenesis. At level, induces myeloid lineage-biased differentiation EZH2-deficiency impairs...
The pig-nosed turtle (Carettochelys insculpta) represents the only extant species within Carettochelyidae family, is a unique Trionychia member fully adapted to aquatic life and currently facing endangerment. To enhance our understanding of this contribute its conservation efforts, we employed high-fidelity (HiFi) Hi-C sequencing technology generate genome assembly at chromosome level. result spans 2.18 Gb, with contig N50 126 Mb, encompassing 34 chromosomes that account for 99.6% genome....
The paper investigates ultimate ruin probability, the probability that time is finite, for an insurance company whose risk reserves follow a Markov-modulated jump–diffusion model. We use both Banach contraction principle and q-scale functions to prove only fixed point of mapping show iterative equation can be employed calculate by algorithm approximating point. Using methodology from Gajek Rudź [(2018). probabilities in regime-switching models. Insurance: Mathematics Economics, 80, 45–53]...
As a matter of fact, Fourier transform is regarded as one the most fundamental tools in image processing recent years, which has been implemented various applications including signal processing, acoustic generation and filtering well processing. With this mind, article, principle transformation introduced discussed. At same time, some related filter techniques to space into frequency space. The specific process four different filters shown study when applied smoothing or sharping graph. To...
Abstract Limbs are a defining characteristic of tetrapods, yet numerous taxa, primarily among amphibians and reptiles, have independently lost limbs as an adaptation to new ecological niches. To elucidate the genetic factors contributing this convergent limb loss, we present 12 Gb chromosome-level assembly Banna caecilian (Ichthyophis bannanicus), limbless amphibian. Our comparative analysis, which includes reconstruction amphibian karyotype evolution, reveals constrained gene length...
ABSTRACT Tissue-specific gene expression requires coordinated control of gene-proximal and -distal cis -regulatory elements (CREs), yet functional analysis gene-distal CREs such as enhancers remains challenging. Here we describe enhanced CRISPR/dCas9-based epigenetic editing systems, enCRISPRa enCRISPRi, for multiplexed enhancer function in situ vivo . Using dual effectors capable re-writing enhancer-associated chromatin modifications, show that enCRISPRi modulate transcription by remodeling...
When an insurance company's risk reserve is governed by a Markov-modulated jump-diffusion model, we study gambler's ruin problem in terms of two-sided probability that the company shall be ruined before its reaches upper barrier level b∈(0,∞). We employ Banach contraction principle and q-scale functions to confirm only fixed point mapping construct iterative algorithm approximating probability. find Lipschitz constant depend on b, premium rate per squared volatility, Markov intensity Poisson...