A5062670552- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Spine and Intervertebral Disc Pathology
- Sarcoma Diagnosis and Treatment
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- Bone Metabolism and Diseases
- MicroRNA in disease regulation
- Musculoskeletal pain and rehabilitation
- Cancer Mechanisms and Therapy
- Acute Myeloid Leukemia Research
- Mycobacterium research and diagnosis
- Ferroptosis and cancer prognosis
- Cancer Immunotherapy and Biomarkers
- Osteoarthritis Treatment and Mechanisms
- Cell death mechanisms and regulation
- PI3K/AKT/mTOR signaling in cancer
- Advanced biosensing and bioanalysis techniques
- Cancer-related Molecular Pathways
- Mesenchymal stem cell research
- PARP inhibition in cancer therapy
- Circular RNAs in diseases
- Electrospun Nanofibers in Biomedical Applications
- Molecular Biology Techniques and Applications
- Cytokine Signaling Pathways and Interactions
Union Hospital
2018-2024
Huazhong University of Science and Technology
2017-2024
Union Hospital
2017-2023
Molecular Oncology (United States)
2020-2023
University of Chicago Medical Center
2020-2023
Wuhan Union Hospital
2017
An increasing number of studies have demonstrated that long non-coding RNAs (lncRNAs) play pivotal roles in cancer onset and development. LncRNA AFAP1-AS1 has been validated to be abnormally upregulated oncogenic various malignant tumors. The biological role mechanism OS (osteosarcoma) remains unclear. Quantitative reverse transcription PCR (qRT-PCR) is applied examine expression tissues cell lines. function cells investigated via in-vitro in-vivo assays. Western bolt rescue experiments are...
Compression-induced apoptosis of nucleus pulposus (NP) cells plays a pivotal role in the pathogenesis intervertebral disc degeneration (IVDD). Recent studies have shown that dysregulation mitochondrial fission and fusion is implicated variety diseases. However, its regulatory effects on compression-induced NP not yet been fully elucidated. Heat shock protein 70 (HSP70) major cytoprotective heat protein, but physiological IVDD, especially effect fusion, still unknown. Herein, we found...
Objective . Excessive apoptosis of nucleus pulposus cells (NPCs) induced by various stresses, including compression, contributes to the development intervertebral disc degeneration (IVDD). Mesenchymal stem (MSCs) can benefit regeneration NPCs and delay IVDD, but underlying molecular mechanism is poorly understood. This study aimed evaluate antiapoptosis effects bone marrow-derived MSC (BMSC) on rat exposed compression investigate whether mitochondrial pathway was involved. Methods. BMSCs...
Purpose: Programmed cell death 1 ligand-1 (PD-L1) and PD-1 as prognostic biomarkers have spurred considerable interest in several types of malignant tumors. In the present meta-analysis, we aimed to elucidate clinicopathological values PD-L1/PD-1 osteosarcoma. Methods: We systematically searched PubMed, Web Science, EMBASE, Scopus, CBM Cochrane Library databases up March 3, 2018. Eligible studies assessing relationship between PD-L1 or expression outcomes osteosarcoma were incorporated....
Skin injury is repaired through a multi-phase wound healing process of tissue granulation and re-epithelialization. Any failure in the may lead to chronic non-healing wounds or abnormal scar formation. Although significant progress has been made developing novel scaffolds and/or cell-based therapeutic strategies promote healing, effective management large skin remains clinical challenge. Keratinocytes are critical re-epithelialization healing. Here, we investigated whether exogenous...
ABSTRACT This study aimed to systematically investigate whether programmed necrosis contributes H 2 O ‐induced nucleus pulposus (NP) cells death and further explore the underlying mechanism involved. Rat NP were subjected different concentrations of for various time periods. The cell viability was measured using a counting kit‐8, rate detected by Hoechst 33258/propidium iodide (PI) staining. necrosis‐related molecules receptor‐interacting protein 1 (RIP1), 3 (RIP3), poly (ADP‐ribose)...
Nucleus pulposus-derived stem/progenitor cells (NPSCs) provide novel prospects for the regeneration of degenerated intervertebral disc (IVD). Nevertheless, with aging and degeneration IVD, frequency NPSCs markedly decreases. Excessive cell death could be main reason declined NPSCs, however, exact mechanisms remain elusive. Thus, present study was undertaken to explore compression-induced death, effects heat shock protein 90 (HSP90) on survival. Here, we found that compression trigger...
Patients with osteosarcoma exhibiting resistance to chemotherapy or presenting metastasis usually have a poor prognosis. Osteosarcoma stem cells (OSCs) are potential cause of tumor metastasis, relapse, and resistance. Therefore, it is necessary develop novel therapeutic drugs, which not only kill but also target OSCs. This study aims explore the anti-osteosarcoma effects Bruceine D (BD), natural compound derived from Brucea javanica, investigate its underlying mechanisms. Results...
Osteosarcoma (OS) is the most common primary malignancy of bone. Epigenetic regulation plays a pivotal role in cancer development various aspects, including immune response. In this study, we studied potential association alterations DNA methylation and transcription immune-related genes with changes tumor microenvironment (TME) prognosis OS. We obtained multi-omics data for OS patients from Therapeutically Applicable Research to Generate Effective Treatments (TARGET) Gene Expression Omnibus...
Abstract Osteosarcoma (OS) is the most common malignant bone tumor. The prognosis of metastatic and recurrent OS patients still remains unsatisfactory. Cisplatin reveals undeniable anti-tumor effect while induces severe side effects that threatening patients’ health. Dynasore, a cell-permeable small molecule inhibits dynamin activity, has been widely studied in endocytosis phagocytosis. However, dynasore on not yet ascertained. In present study, we suggested inhibited cell proliferation,...
RNA interference (RNAi) is mediated by an ∼21-nt double-stranded small interfering (siRNA) and shows great promise in delineating gene functions developing therapeutics for human diseases. However, effective silencing usually requires the delivery of multiple siRNAs a given gene, which often technically challenging time-consuming. In this study, exploiting type IIS restriction endonuclease-based synthetic biology methodology, we developed fast assembly multiplex (FAMSi) system. our...
Adenovirus (Ad) is a non-enveloped linear double-stranded DNA virus with >50 serotypes in humans. Ad vectors have been used as gene delivery vehicles to express transgenes, small interfering RNAs (siRNAs) for silencing, or CRISPR/Cas and designer nucleases genome editing. Although several methods are generate vectors, the Ad-making process remains technically challenging time consuming. Moreover, techniques not improved past two decades. Gibson Assembly (GDA) technology allows one-step...
Plasmid DNA (pDNA) isolation from bacterial cells is one of the most common and critical steps in molecular cloning biomedical research. Almost all pDNA purification involves disruption bacteria, removal membrane lipids, proteins genomic DNA, bulk lysate, concentration for downstream applications. While many liquid-phase solid-phase methods are used, final preparations usually contaminated with varied degrees host RNA, which cannot be completely digested by RNase A. To develop a simple,...
Aging | doi:10.18632/aging.203232. Linjuan Huang, Ling Zhao, Jing Zhang, Fang He, Hao Wang, Qing Liu, Deyao Shi, Na Ni, William Wagstaff, Connie Chen, Russell R. Reid, Rex C. Haydon, Hue H. Luu, Le Shen, Tong-Chuan Liangdan Tang
Background: Lycorine, a natural alkaloid, has been indicated to have various physiological effects, including potential effect against cancer. However, the anticancer of lycorine on osteosarcoma (OS) and detailed molecular mechanisms involved remain unclear. Purpose: The purpose this study was examine human OS elucidated it underlying Materials methods: In vitro assays, cells were treated with at concentrations. Then cell proliferation, colony formation, cycle distribution, apoptosis,...