A5101750113- Acute Myeloid Leukemia Research
- Advanced biosensing and bioanalysis techniques
- Ferroptosis and cancer prognosis
- Cancer Genomics and Diagnostics
- Cancer, Lipids, and Metabolism
- Hemoglobinopathies and Related Disorders
- Biosensors and Analytical Detection
- Connective Tissue Growth Factor Research
- Bone and Dental Protein Studies
- TGF-β signaling in diseases
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Acute Lymphoblastic Leukemia research
- Tuberculosis Research and Epidemiology
- Cancer-related gene regulation
- COVID-19 Clinical Research Studies
- Chronic Myeloid Leukemia Treatments
- Nuclear Receptors and Signaling
- SARS-CoV-2 detection and testing
- Long-Term Effects of COVID-19
- Hedgehog Signaling Pathway Studies
- Signaling Pathways in Disease
- Circular RNAs in diseases
- Kruppel-like factors research
First People's Hospital of Chongqing
2017-2025
Chongqing Medical University
2024-2025
Chongqing University
2024
University of Chicago Medical Center
2019-2023
Molecular Oncology (United States)
2008-2023
University of Chinese Academy of Sciences
2020-2023
Chongqing University of Arts and Sciences
2022
National Natural Science Foundation of China
2018
Southwest Hospital
2014
Army Medical University
2012-2014
Abstract Background We aim to investigate the profile of acute antibody response in COVID-19 patients, and provide proposals for usage test clinical practice. Methods A multi-center cross-section study (285 patients) a single-center follow-up (63 were performed feature SARS-CoV-2. cohort 52 suspects 64 close contacts enrolled evaluate potentiality test. Results The positive rate IgG reached 100% around 20 days after symptoms onset. median day seroconversion both lgG IgM was 13 Seroconversion...
Mesenchymal stem cells (MSCs) are multipotent progenitors that have the ability to differentiate into multiple lineages, including bone, cartilage, and fat. We previously demonstrated least known bone morphogenetic protein (BMP)9 (also as growth differentiation factor 2) is one of potent osteogenic factors can induce both adipogenic MSCs. Nonetheless, molecular mechanism underlying BMP9 action remains be fully understood. Leptin an adipocyte-derived hormone in direct proportion amount body...
COVID-19 infections are still at pandemic levels globally and there currently no specific drugs to treat these infections. Previous studies have demonstrated that serum albumin were abnormally low in patients might be used as a prognosis biomarker. Supplemental has been an experimental therapeutic method. However, dynamic evaluation of with was limited whether could predict the is unknown.We enrolled 79 present study reviewed electronic medical laboratory records. Data processed using SPSS...
Bone morphogenetic protein 9 (BMP9) (or GDF2) was originally identified from fetal mouse liver cDNA libraries. Emerging evidence indicates BMP9 exerts diverse and pleiotropic functions during postnatal development in maintaining tissue homeostasis. However, the expression landscape of signaling and/or adult tissues remains to be analyzed. Here, we conducted a comprehensive analysis its mediators mice. By analyzing ENCODE transcriptome datasets found Bmp9 highly expressed detectable embryonic...
Understanding the bone and musculoskeletal system is essential to maintain health quality of life our aging society. Mesenchymal stem cells (MSCs) can undergo self-renewal differentiate into multiple tissue types including bone. We demonstrated that BMP9 most potent osteogenic factors although molecular mechanism underlying action not fully understood. Long noncoding RNAs (lncRNAs) play important regulatory roles in many physiological and/or pathologic processes. Here, we investigated role...
ABSTRACT Objective Lipid metabolic reprogramming is closely intertwined with the development and progression of thyroid carcinoma (TC); however, its specific mechanism remains elusive. This study aimed to investigate association between lipid metabolism TC progression. Methods We employed liquid chromatography‐mass spectrometry (LC/MS) for an untargeted metabolomics analysis, comparing 12 patients healthy controls (HC). Additionally, we conducted screening differentially expressed genes...
RNA interference (RNAi) is mediated by an ∼21-nt double-stranded small interfering (siRNA) and shows great promise in delineating gene functions developing therapeutics for human diseases. However, effective silencing usually requires the delivery of multiple siRNAs a given gene, which often technically challenging time-consuming. In this study, exploiting type IIS restriction endonuclease-based synthetic biology methodology, we developed fast assembly multiplex (FAMSi) system. our...
Plasmid DNA (pDNA) isolation from bacterial cells is one of the most common and critical steps in molecular cloning biomedical research. Almost all pDNA purification involves disruption bacteria, removal membrane lipids, proteins genomic DNA, bulk lysate, concentration for downstream applications. While many liquid-phase solid-phase methods are used, final preparations usually contaminated with varied degrees host RNA, which cannot be completely digested by RNase A. To develop a simple,...
RNA sequencing (RNA-seq)-based whole transcriptome analysis (WTA) using ever-evolving next-generation technologies has become a primary tool for coding and/or noncoding profiling. As WTA requires RNA-seq data both and RNAs, one key step obtaining high-quality is to remove ribosomal which can be accomplished by various commercial kits. Nonetheless, an ideal rRNA removal method should efficient, user-friendly cost-effective so it adapted homemade library construction. Here, we developed novel...
CCN1 plays distinct roles in various tumor types, but little is known regarding the role of leukemia.We analyzed protein expression leukemia cell lines and AML bone marrow samples. We also evaluated effects antibody- or siRNA-mediated inhibition on growth two (U937 Kasumi-1 cells) MEK/ERK pathway, β-catenin other related genes.U937 cells had markedly higher than 5 lines, was samples normal Blocking with an antibody U937 suppressed proliferation, increased apoptosis, down-regulated Bcl-xL...
In this work, we constructed a novel electrochemiluminescent (ECL) strategy based on sandwich immunoassay-induced target transformation assisted with catalyzed hairpin assembly (CHA) amplification for ultrasensitive bioassay cysteine-rich protein 61 (CCN1) as model. First, the CCN1 could be equally transformed into specific oligonucleotide (initiator I) labeled detection antibody immunoassay. addition, initiator I triggered an efficient nonenzymatic CHA in presence of ferrocene-labeled 1...
Intestinal cancers are developed from intestinal epithelial stem cells (ISCs) in crypts through a multi-step process involved genetic mutations of oncogenes and tumor suppressor genes. ISCs play key role maintaining the homeostasis gut epithelium. In 2009, Sato et al established three-dimensional culture system, which mimicked niche microenvironment by employing factors, successfully grew crypt into organoids or Mini-guts vitro. Since then, organoid technology has been used to delineate...
Abstract Objective To explore the optimal models for predicting formation of high-quality embryos in Poor Ovarian Response (POR) Patients with Progestin-Primed Stimulation (PPOS) using machine learning algorithms. Methods A retrospective analysis was conducted on clinical data 4,216 POR cycles who underwent vitro fertilization (IVF) / intracytoplasmic sperm injection (ICSI) at Sichuan Jinxin Xinan Women and Children’s Hospital from January 2015 to December 2021. Based presence cleavage 72 h...
γ-catenin plays different roles in types of tumors, and its role chronic myeloid leukemia (CML) cells has yet to be identified. In our study, two CML cell lines (K562, KU812) had higher expression levels compared five BCR-ABL-negative cells. Knockdown the BCR-ABL resulted downregulation γ-catenin. Furthermore, by siRNA inhibited proliferation colony formation c-Myc cyclin D1 genes; also potentiated effects imatinib (inhibiting inducing apoptosis) suppressed anti-apoptotic genes Bcl-xL...
Introduction: Moraxella catarrhalis , which is an opportunistic pathogen and one of the three major pathogens community-acquired pneumonia, causes a variety infections in clinic. In recent years, isolation rate has gradually increased. China, due to clinical empirical use antibiotics, resistance isolated from children β-lactam antibiotics reached 99%. The non-susceptible macrolide also increased significantly. Methods: Two isolates (R17123922_R R18013231_R) were in-patients confirmed be...
Purpose: There is growing evidence that the immune system plays an important role in progression of Parkinson's disease, second most common neurodegenerative disorder. This study aims to address comprehensive understanding immunopathogenesis disease and explore new inflammatory biomarkers. Patients Methods: In this study, Immune-related differential expressed genes (DEIRGs) were obtained from GEO database Immport database. The hub gene was screened DEIRGs using LASSO regression random forest...
Acute myeloid leukemia (AML), a disease formed by mutations in pluripotent stem cells or mildly differentiated precursor cells, is clonal malignant of the hematopoietic system. In recent years, with application immunology, cytogenetics, and molecular biology, deeper understanding biological properties AML tumor has laid foundation for accurate classification, diagnosis, prognosis, selection optimal treatment AML. This article reviewed relevant literature on difference clinical diagnosis...