Previous studies have suggested that microRNAs (miRNAs/miRs) may positively or negatively control osteogenic differentiation and mineralization by targeting negative regulators of osteogenesis important factors. miR‑21 is in osteoblast Smad7 a critical regulator differentiation, which inhibits proliferation, mouse cells. However, the association between remain to be elucidated. In present study, was found promote level increase matrix MC3T3‑E1 Furthermore, identified as direct target The...

Cryogenic cluster beam experiments have provided crucial insights into the evolution of metallic state from atom to bulk. Surprisingly, one most fundamental properties, ability a metal efficiently screen electric fields, is still poorly understood in small clusters. Theory has predicted that many Na clusters are unable charge inhomogeneities and thus permanent dipole moments. High precision deflection on cryogenically cooled ${\mathrm{Na}}_{N}$ ($N<200$) show moments at least an order...

Neointima formation causes the failure of 60% arteriovenous fistulas (AVFs) within 2 years. Neointima-forming mechanisms are controversial but possibly linked to excess proinflammatory responses and dysregulated Notch signaling. To identify how AVFs fail, we anastomosed carotid artery internal jugular vein in normal uremic mice compared these findings with those failed from patients ESRD. Endothelial cells (ECs) or expressed mesenchymal markers (FSP-1 and/or α-SMA) exhibited increased...

Hemodialysis patients depend on arteriovenous fistulas (AVF) for vascular access. Unfortunately, their 2-yr primary patency rate is only 60% because of AVF clog due to intimal hyperplasia at the venous anastomosis. Chronic kidney disease (CKD) can increase neointima formation by unknown mechanisms. A new mouse model was created, and mechanisms CKD in AVFs were investigated. We created mice anastomosing common carotid artery internal jugular vein. induced [BUN (blood urea nitrogen) control...

Insulin-like growth factor-1 receptor (IGF-1R) can regulate vascular homeostasis and endothelial function. We studied the role of IGF-1R in oxidative stress-induced dysfunction. Unilateral ureteral obstruction (UUO) was performed wild-type (WT) mice with cell (EC)-specific knockout (KO). After UUO KO mice, barrier dysfunction more severe than WT as seen by increased inflammatory infiltration (VE)-cadherin phosphorylation. interstitial fibroblast accumulation enhanced extracellular protein...

Rationale: Mechanical stress plays an important role in proliferation of venous smooth muscle cells (SMCs) neointima, a process formation that contributes to failure vein grafts. However, it is unknown what intracellular growth signal leads SMCs. Objective: The objective this study identify mechanisms mechanical stretch on neointima formation. Methods and Results: By microarray analysis, we found cyclic (15% elongation) stimulated the transcription SGK-1 (serum-, glucocorticoid-regulated...

Fibroblast-specific protein 1 (FSP-1) plays multiple roles in promoting cell proliferation and motility. Increased FSP-1 expression smooth muscle cells (SMCs) has been associated with their enhanced proliferation.To study how contributes to neointima formation of vein grafts.Arteriovenous grafts were created wild-type or FSP-1-GFP mice (green fluorescent regulated by promoter). The effects on bone marrow (BM) migration SMC studied vivo vitro.On creation a graft, there was rapid deposition...

Muscle proteolysis in CKD is stimulated when the ubiquitin-proteasome system activated. Serum glucocorticoid–regulated kinase 1 (SGK-1) involved skeletal muscle homeostasis, but role of this protein CKD–induced wasting unknown. We found that, compared with muscles from healthy controls, patients and mice express low levels SGK-1. In mice, SGK-1-knockout (SGK-1-KO) induced loss that correlated increased expression ubiquitin E3 ligases known to facilitate degradation by ubiquitin-proteasome,...

Background/Aims: Neointimal hyperplasia is responsible for stenosis, which requires corrective vascular surgery, and also a major morphological feature of many cardiovascular diseases. This involves the endothelial-to-mesenchymal transition (EndMT). We investigated whether integrin β3 can modulate EndMT, as well its underlying mechanism. Methods: Integrin was overexpressed or knocked down in human umbilical vein endothelial cells (HUVECs). The expression markers mesenchymal determined by...

Glutathione transferase isozyme A4 (GSTA4) exhibits high catalytic efficiency to metabolize 4-hydroxynonenal (4-HNE), a highly reactive lipid peroxidation product that has been implicated in the pathogenesis of various chronic diseases. We investigated role 4-HNE mechanisms unilateral ureteral obstruction (UUO)-induced fibrosis and its modulation by GSTA4-4 mouse model. Our data indicate after UUO, accumulation adducts were increased renal tissues, with concomitant decrease expression mice....

The present study was performed to compare the clinical efficacy of lateral plate and medial double-plating fixation distal femoral fractures explore indication fractures. From March 2006 April 2014, 48 12 cases were treated with (single plate) plates (double plates), respectively. During surgery, after setting for fractures, if varus stress test knee positive collateral ligament rupture excluded, used stability fragments. All patients followed up at an average period 15.9 months. operation...

Stern–Gerlach deflection measurements have been performed on rare earth clusters TbN, HoN, and TmN (N≤40) at cryogenic temperatures (T≤77 K). TbN HoN share a common size dependence in their magnetic moments. They both exhibit “magic number” sizes which show reduced net moments, similar to previous observations for Gd Dy clusters. smaller moments that do not differ significantly between cluster sizes. The are evidence the atomic canceled by canted or antiferromagnetic alignment. Electric...

Objective— Neointima formation is associated with stenosis and subsequent thrombosis in arteriovenous grafts (AVGs). A role of integrin β3 the neointima AVGs remains poorly understood. Approach Results— In −/− mice, we found significantly accelerated occlusion compared wild-type mice. This caused by development lack endothelial regeneration. The latter a direct consequence impaired functions circulating angiogenic cells (CACs) platelets Evidence suggests involvement platelet regulating CAC...

The magnetic moments and electric dipoles of Tb Pr clusters are investigated using the Stern–Gerlach deflection technique. addition a single oxygen atom induces an increase in dipole TbN clusters, however moment is largely not affected. In neither nor This raises questions as to role conduction electrons exchange interaction rare earth puts into doubt validity Ruderman-Kittel-Kasuya-Yosida (RKKY) mechanism small systems.

Abstract Objective To explore the entry point, orientation, and fixation range of retrograde acetabular posterior column screw. Method The computed tomography data 100 normal adult pelvises (50 males 50 females, respectively) were collected pelvis three-dimensional (3D) reconstruction was performed by using Mimics software 3D model imported into Geomagic Studio software. perspective carried out orienting from ischial tuberosity to iliac fossa in Virtual screw inserted perpendicular...

<title>Abstract</title> Objective The aim of this study is to compare the efficacy plate fixation via tarsal sinus approach with screw alone in treating Sander type II, III, and IV fractures. Method Between 2016 2023, a comprehensive encompassing 73 patients 78 feet calcaneus fractures was conducted. investigation compared open reduction (ORPF) (18 cases) against (ORSF) (60 cases). Parameters such as operation duration, intraoperative blood loss, hospital stay postoperative imaging metrics...

Diagram of the construction, release, and delivery performance CLAP@CS/γ-PGA.

With the rapid growth of digital information, personalized recommendation systems have become an indispensable part Internet services, especially in fields e-commerce, social media, and online entertainment. However, traditional collaborative filtering content-based methods limitations dealing with data sparsity cold start problems, face largescale heterogeneous data, which makes it difficult to meet user expectations. This paper proposes a new label algorithm based on metric learning, aims...