A5070093051- Atherosclerosis and Cardiovascular Diseases
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Cardiovascular Health and Disease Prevention
- Single-cell and spatial transcriptomics
- Peripheral Artery Disease Management
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Cardiovascular Disease and Adiposity
- Cerebrovascular and Carotid Artery Diseases
- Antiplatelet Therapy and Cardiovascular Diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Immune cells in cancer
- Pregnancy and preeclampsia studies
- Cardiac Imaging and Diagnostics
- Lipoproteins and Cardiovascular Health
- Bioinformatics and Genomic Networks
- Galectins and Cancer Biology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Acute Ischemic Stroke Management
- Venous Thromboembolism Diagnosis and Management
- Caveolin-1 and cellular processes
- Medication Adherence and Compliance
- RNA Research and Splicing
- Vascular Procedures and Complications
University Medical Center Utrecht
2023-2025
Utrecht University
2023-2024
Radboud University Nijmegen
2024
Radboud University Medical Center
2024
Oxidized phospholipids play a key role in the atherogenic potential of lipoprotein(a) (Lp[a]); however, Lp(a) is complex particle that warrants research into additional proinflammatory mediators. We hypothesized Lp(a)-associated lipids contribute to atherogenicity Lp(a).
Purpose The Utrecht Cardiovascular Cohort–Second Manifestations of Arterial Disease (UCC-SMART) Study is an ongoing prospective single-centre cohort study with the aim to assess important determinants and prognosis cardiovascular disease progression. This article provides update rationale, design, included patients, measurements findings from start in 1996 date. Participants UCC-SMART includes patients aged 18–90 years referred University Medical Center Utrecht, Netherlands, for management...
Epigenetic age estimators (clocks) are predictive of human mortality risk. However, it is not yet known whether the epigenetic atherosclerotic plaques for risk cardiovascular events.
Women presenting with coronary artery disease more often present fibrous atherosclerotic plaques, which are currently understudied. Phenotypically modulated smooth muscle cells (SMCs) contribute to atherosclerosis in women. How these phenotypically SMCs shape female versus male plaques is unknown.
Systemic low-grade inflammation, measured by plasma high-sensitivity C-reactive protein (hsCRP) levels, is an important risk factor for atherosclerotic cardiovascular disease (ASCVD). To date, however, it unknown whether hsCRP associated with adverse histological plaque features.
Abstract Background and Aims Metabolism dictates macrophage function plays a central role in atherosclerotic plaque progression. The kynurenine pathway, which metabolizes the majority of essential amino acid tryptophan, pivotal regulating immune responses supporting NAD+ synthesis, for cellular energy metabolism. Higher circulating levels are associated with cardiovascular disease, yet their plaques is unclear. This study aims to investigate underlying mechanisms driving increased...
Macrophage influx is an important feature of human plaque destabilisation. Studies in mice suggest a role the isoD(Asp)G(Gly)R(Arg) three amino acid motif fibronectin (isoDGR fibronectin) macrophage influx. The association isoDGR plasma and with vulnerable characteristics has not been investigated large cohorts. Levels were measured individual plaques from carotid endarterectomy (CEA) patients Athero-Express biobank associated content other same patient. using enzyme linked immunosorbent...
Abstract Atherosclerosis is a chronic inflammatory disease which driven in part by the aberrant trans -differentiation of vascular smooth muscle cells (SMCs). No therapeutic drug has been shown to reverse detrimental SMC-derived cell phenotypes into protective phenotypes, hypothesized enabler plaque regression and improved patient outcome. Herein, we describe novel function colchicine beneficial modulation phenotype, independent its conventional anti-inflammatory effects. Using SMC fate...
Sex differences in atherosclerosis have been described with female plaques being mostly perceived as stable and fibrous. Sex-specific mechanisms such mosaic loss of the Y chromosome men linked to cardiovascular health. In women, X-linked X inactivation (XCI) skewing is common several tissues. Yet, information on role XCI atherosclerotic lacking. Here, we investigated presence advanced lesions its association risk factors, histological plaque data, clinical data. was quantified 154 55 blood...
Introduction The risk of major bleeding complications in catheter directed thrombolysis (CDT) for acute limb ischemia (ALI) remains high, with reported complication rates up to 1 every 10 treated patients. Fibrinogen was the only predictive marker used CDT, despite lack high quality evidence support this. Therefore, recent international guidelines recommend against use fibrinogen during CDT. However, no alternative biomarkers exist effectively predict CDT-related complications. aim POCHET...
Background Plaque myofibroblasts are critical players in the initiation and advancement of atherosclerotic disease. They involved production extracellular matrix, formation fibrous cap, underlying lipidic core via modulation processes response to different environmental cues. Despite clear phenotypic differences between myofibroblast cells healthy vascular smooth muscle cells, still widely used as a cellular model research. Methods Results Here, we present conditioned outgrowth method...
Abstract Women presenting with coronary artery disease (CAD) more often present fibrous atherosclerotic plaques, which are currently understudied. Phenotypically modulated smooth muscle cells (SMCs) contribute to atherosclerosis in women. How these phenotypically SMCs shape female versus male plaques is unknown. Here, we show sex-stratified gene regulatory networks (GRNs) from human carotid tissue. Prioritization of identified two main SMC GRNs late-stage atherosclerosis. Single-cell...
Abstract Epigenetic age estimators (clocks) are known to be predictive of human mortality risk. However, it is not yet whether the epigenetic atherosclerotic plaques can used for predicting secondary events. Here we estimated an adjusted measure age, acceleration (EAA), using DNA methylation and blood. EAA plaque, but blood, independently predicted events in a 3-year follow-up (HR=1.3, p= 0.018). Plaque concurred with high metabolic transcriptional state plaques. Patients diabetes body mass...
Abstract Funding Acknowledgements Type of funding sources: Public grant(s) – National budget only. Main source(s): Dutch Research Council Veni grant. Netherlands Heart Foundation Atherosclerosis is characterized by inflammation and lipid accumulation, leading to complications such as stroke myocardial infarction. Various macrophage subsets are present in human atherosclerotic plaques. However, it unclear how interrelated contribute the disease process its clinical outcomes. Here, we employed...
Abstract Background Previously we reported a time dependent change in atherosclerotic carotid plaque characteristics, with decreasing destabilising characteristics between 2002 -2011. This observation was considered confirmative parallel improved adherence to medication and decrease overall cardiovascular mortality Western Europe. In the present study, investigated if these changes still occurred over last decade. Methods Using Athero Express biobank, plaques of 1,277 consecutive...
Objective: Deamidation of the NGR (Asn-Gly-Arg) motif to isoDGR (isoAsp-Gly-Arg) in fibronectin (IsoDGR-fibronectin) enhances vitro monocyte and endothelial cell activation. Blocking reduces macrophage influx murine tissues. Although is an important feature human plaque destabilization, role for plasma isoDGR-fibronectin atherosclerotic thereby increasing vulnerability has not been investigated large cohorts. Design: IsoDGR-fibronectin levels plaques were measured carotid endarterectomy...
Abstract Background Sex and plaque histology are intertwined, with fibrous atherosclerotic plaques being more prevalent in women pointing to general smooth muscle cell plasticity estrogen signaling. Plaque erosion, a significant contributor acute coronary syndromes (ACSs), is linked as compared men. We hypothesize that the molecular drivers of histologically determined differ between men women. Methods Human end-stage were isolated from consecutive patients who underwent carotid...
Abstract Plaque smooth muscle cells are critical players in the initiation and advancement of atherosclerotic disease. They produce extracellular matrix (ECM) components, which play a role lesion progression stabilization. Despite clear phenotypic differences between plaque vascular (VSMCs), VSMCs still widely used as model system research. Here we present conditioned outgrowth method to isolate cells. We obtained from 27 donors (24 carotid 3 femoral endarterectomies). show that these keep...
Abstract Background and aim: Sex differences in atherosclerosis have been described with female plaques being mostly perceived as stable fibrous. Sex-specific mechanisms such mosaic loss of the Y chromosome men linked to cardiovascular health. In women, X-linked X inactivation (XCI) skewing is common several tissues. Yet, information on role XCI atherosclerotic lacking. Here, we investigated presence advanced lesions its association risk factors, histological plaque data, clinical data....