The mechanism(s) of how bacteria acquire tolerance and then resistance to antibiotics remains poorly understood. Here, we show that glucose abundance decreases progressively as ampicillin-sensitive strains ampicillin. mechanism involves ampicillin initiates this event via targeting pts promoter pyruvate dehydrogenase (PDH) promote transport inhibit glycolysis, respectively. Thus, fluxes into pentose phosphate pathway generate reactive oxygen species (ROS) causing genetic mutations....

Sodium-translocating NADH:quinone oxidoreductase (Na+-NQR) functions as a unique redox-driven sodium pump, generating membrane potential, which is related to aminoglycoside antibiotic resistance. However, whether it modulates other metabolisms confer resistance unknown. The present study showed that loss of nqrA or nqrF led differential metabolomes with elevated antibiotics. Decreased alanine, aspartate, and glutamate metabolism depressed abundance alanine were characterized the most...

Misuse and overuse of antibiotics drive the selection spread antibiotic-resistant bacteria. Although genetic mutations have been well defined for different types antibiotic resistance, ways to revert resistance are largely unexplored. Here, we adopted a proteomics approach investigate mechanism underlying ciprofloxacin in Edwardsiella tarda, representative pathogen that infects both economic animal species human beings. By comparing protein expression profiles ciprofloxacin-sensitive...

Infections with Pseudomonas aeruginosa have become a real concern among hospital-acquired infections, especially in cystic fibrosis patients and immunocompromised individuals. Control of the pathogen is challenging due to antibiotic resistance.

The present study explored the cooperative effect of both alanine (Ala) and gentamicin (Gent) on metabolic mechanisms by which exogenous Ala potentiates Gent to kill antibiotic-resistant Vibrio alginolyticus. To test this, GC-MS-based metabolomics was used characterize Ala-, Gent- both-induced profiles, identifying nitric oxide (NO) production pathway as most key clue understand mechanisms. Gent, led low, lower lowest activity total synthase (tNOS) level NO, respectively. NOS promoter...

Antibiotic-resistant Pseudomonas aeruginosa has become a real concern in hospital-acquired infections, especially critically ill and immunocompromised patients. Understanding antibiotic resistance mechanisms developing novel control measures are highly appreciated.

Non-inheritable antibiotic or phenotypic resistance ensures bacterial survival during treatment. However, exogenous factors promoting are poorly defined. Here, we demonstrate that Vibrio alginolyticus recalcitrant to killing by a broad spectrum of antibiotics under high magnesium. Functional metabolomics demonstrated magnesium modulates fatty acid biosynthesis increasing saturated while decreasing unsaturated production. Exogenous supplementation and acids increased decreased susceptibility...

The metabolic environment is responsible for antibiotic resistance, which highlights the way in resistance mechanism works. Here, GC-MS-based metabolomics with iTRAQ-based proteomics was used to characterize a state tetracycline-resistant Escherichia coli K12 (E. coli-RTET) compared tetracycline-sensitive E. K12. repressed pyruvate cycle against elevation of proton motive force (PMF) and ATP constructed most characteristic feature as consequence tetracycline resistance. To understand role...

Non-inheritable antibiotic or phenotypic resistance ensures bacterial survival during treatment. However, exogenous factors promoting are poorly defined. Here, we demonstrate that Vibrio alginolyticus recalcitrant to killing by a broad spectrum of antibiotics under high magnesium. Functional metabolomics demonstrated magnesium modulates fatty acid biosynthesis increasing saturated while decreasing unsaturated production. Exogenous supplementation and acids increased decreased susceptibility...

Rhodococcus ruber with organic tolerance has potential applications in biotransformation and bioremediation. To explore the possible mechanism, response of R. SD3 to toluene phenol was investigated using a quantitative proteomics approach isobaric tag for relative absolute quantification (iTRAQ) liquid chromatography-tandem mass spectrometry. A total 362 488 differentially expressed proteins were identified treatment group as compared control group, respectively. Functional annotation...

Metabolic reprogramming is one of the hallmarks a tumor. It not only promotes development and progression tumor but also contributes to resistance cells chemotherapeutics. The difference in metabolism between drug-resistant sensitive indicates that have experienced metabolic adaptation. response induced by chemotherapy dynamic, early anticancer drugs effect an initial on drug been well studied. Early intervention may prevent or slow down resistance. differential responses normal are unclear....

Bacterial infections cause huge losses in aquaculture and a wide range of health issues humans. A vaccine is the most economical, efficient, environment-friendly agent for protecting hosts against bacterial infections. This study aimed to identify broad, cross-protective antigens from extracellular secretory proteome marine bacterium Vibrio alginolyticus . Of 69 predicted proteins its genome, 16 were randomly selected gene cloning construct DNA vaccines, which used immunize zebrafish (Danio...

Because, as of yet, there are few new antibiotics active against multidrug-resistant bacteria being explored, compounds including metabolites that might help us tide over this crisis greatly expected. A recently adopted method to evaluate the potentiation is plate-counting test. However, time-consuming, strenuous, and unfeasible for a large scale screening. minimum inhibitory concentration (MIC) test by using microtitre plate dilution convenient economic identification, but it cannot be used...

Abstract Non-inheritable antibiotic or phenotypic resistance ensures bacterial survival during treatment. However, exogenous factors promoting are poorly defined. Here, we demonstrate that Vibrio alginolyticus recalcitrant to killing by a broad spectrum of antibiotics under high magnesium. Functional metabolomics demonstrated magnesium modulates fatty acid biosynthesis increasing saturated while decreasing unsaturated production. Exogenous supplementation and acids increased decreased...

Non-inheritable antibiotic resistance or phenotypic ensures bacterial survival upon treatment. However, exogenous factors in promoting is poorly defined. Here, we demonstrate that Vibrio alginolyticus are recalcitrant to killing by a broad spectrum of antibiotics under high magnesium. Functional metabolomics magnetism modulates the biosynthesis fatty acids increasing saturated while decreasing unsaturated acids. Exogenous supplementation confirm role resistance. Furthermore, functional...

Non-inheritable antibiotic or phenotypic resistance ensures bacterial survival during treatment. However, exogenous factors promoting are poorly defined. Here, we demonstrate that Vibrio alginolyticus recalcitrant to killing by a broad spectrum of antibiotics under high magnesium. Functional metabolomics demonstrated magnesium modulates fatty acid biosynthesis increasing saturated while decreasing unsaturated production. Exogenous supplementation and acids increased decreased susceptibility...