A5033678900- Aquaculture disease management and microbiota
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Metabolomics and Mass Spectrometry Studies
- Bacteriophages and microbial interactions
- Invertebrate Immune Response Mechanisms
- Protein Structure and Dynamics
- Antibiotics Pharmacokinetics and Efficacy
- Immune Response and Inflammation
- Escherichia coli research studies
- Aquaculture Nutrition and Growth
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial biofilms and quorum sensing
- Gut microbiota and health
- RNA and protein synthesis mechanisms
- Hepatitis B Virus Studies
- interferon and immune responses
- Enzyme Structure and Function
- Advanced Proteomics Techniques and Applications
- Identification and Quantification in Food
- Genomics and Phylogenetic Studies
- Hepatitis C virus research
- Monoclonal and Polyclonal Antibodies Research
- Salmonella and Campylobacter epidemiology
Nanchang University
1996-2025
State Key Laboratory of Food Science and Technology
2025
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)
2019-2024
Sun Yat-sen University
2015-2024
Qingdao National Laboratory for Marine Science and Technology
2017-2024
Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai)
2019-2022
Third Affiliated Hospital of Sun Yat-sen University
2016-2020
Jinan University
2016
First Affiliated Hospital of Jinan University
2016
City University
2014
Significance Exogenous metabolites have been documented to potentiate antibiotics kill multidrug-resistant pathogens, but the mechanisms are largely unknown. The work presented here shows that intermetabolites from TCA cycle and phosphoenolpyruvate (PEP)-pyruvate-AcCoA pathway potential improve targeting of these resistant microorganisms. enzymes connect PEP-pyruvate-AcCoA essential for this potentiation, indicating both can be merged considered a pyruvate (P cycle). P operates routinely as...
Serum resistance is a poorly understood but common trait of some difficult-to-treat pathogenic strains bacteria. Here, we report that glycine, serine and threonine catabolic pathway down-regulated in serum-resistant Escherichia coli, whereas exogenous glycine reverts the serum effectively potentiates to eliminate clinically-relevant bacterial pathogens vitro vivo. We find increases formation membrane attack complex on through two previously unrecognized regulations: 1) negatively positively...
Multidrug-resistant Escherichia coli metabolomes are reprogrammed by glutamine to be sensitive ampicillin in vitro and vivo.
The mechanism(s) of how bacteria acquire tolerance and then resistance to antibiotics remains poorly understood. Here, we show that glucose abundance decreases progressively as ampicillin-sensitive strains ampicillin. mechanism involves ampicillin initiates this event via targeting pts promoter pyruvate dehydrogenase (PDH) promote transport inhibit glycolysis, respectively. Thus, fluxes into pentose phosphate pathway generate reactive oxygen species (ROS) causing genetic mutations....
Soil rhizospheric metaproteomics is a powerful scientific tool to uncover the interactions between plants and microorganisms in soil ecosystem. The present study established an extraction method suitable for different soils that could increase extracted protein content. Close 1000 separate spots with high reproducibility be identified stained 2-DE gels. Among spots, 189 representing 122 proteins on gel of rice samples were successfully by MALDI-TOF/TOF-MS. These mainly originated from...
Edwardsiella tarda, the causative agent of Edwardsiellosis, imposes medical challenges in both clinic and aquaculture. The emergence multidrug resistant strains makes antibiotic treatment impractical. identification molecules that facilitate or promote efficacy is high demand. In present study, we aimed to identify small whose abundance correlated with kanamycin resistance E. tarda by gas chromatography–mass spectrometry. We found fructose was greatly suppressed kanamycin-resistant strains....
Strategy of managing antibiotic-resistant Vibrio alginolyticus, a bacterial pathogen that threatens human health and animal farming, is not available due to the lack knowledge about underlying mechanism antibiotic resistance. Here, we showed gentamicin-resistant V. alginolyticus (VA-RGEN ) has four mutations on metabolism one mutation two-component system by whole-genome PCR-based sequencing, indicating metabolic shift in VA-RGEN. Thus, profile was investigated GC-MS based metabolomics....
Macrophages restrict bacterial infection partly by stimulating phagocytosis and release of cytokines complement components. Here, we treat macrophages with LPS a pathogen, demonstrate that expression cytokine IL-1β increase to transient peak 8 12 h post-treatment, while component 3 (C3) continues rise for 24 post-treatment. Metabolomic analysis suggests correlation between the cellular concentrations succinate inosine C3. This may involve regulatory feedback mechanism, whereby stimulates...
The elucidation of the molecular details antibiotic resistance will lead to improvements in extending efficacy current antimicrobials. In study, proteomic methodologies were applied characterize functional outer membrane proteins (Omps) E. coli K-12 responded tetracycline and ampicillin for understanding universal pathways that form barriers antimicrobial agents. For this purpose, expressional proteome was characterized identified with use 2-DE MALDI-TOF/MS methods. Then, differential Omps...
Bacterial antibiotic resistance has become a worldwide challenge with the overuse and misuse of drugs.
Vibrio alginolyticus is a waterborne pathogen that infects wide variety of hosts including fish and human, the outbreak this can cause huge economic loss in aquaculture. Thus, enhancing host's capability to survive from V. infection key fighting remains still unexplored. In present study, we established alginolyticus-zebrafish interaction model by which explored how zebrafish survived infection. We used GC-MS based metabolomic approaches characterize differential metabolomes between survival...
Colistin is a last-line antibiotic against Gram-negative multidrug-resistant bacteria, but the increased resistance poses huge challenge to this drug. However, mechanisms underlying such are largely unexplored. The present study first identified mutations of two genes encoding AceF subunit pyruvate dehydrogenase (PDH) and TetR family transcriptional regulator in colistin-resistant Vibrio alginolyticus (VA-RCT ) through genome sequencing. Then, gas chromatography-mass spectroscopy-based...
Summary Antibiotic‐resistant Vibrio alginolyticus poses a big challenge to human health and food safety. It is urgently needed understand the mechanisms underlying antibiotic resistance develop effective approaches for control. Here we explored metabolic difference between gentamicin‐resistant V . (VA‐R GEN ) gentamicin‐sensitive (VA‐S), found that reactive oxygen species (ROS) generation was altered. Compared with VA‐S, ROS content in VA‐R reduced due decreased increased breakdown of ROS....
Development of low-cost and eco-friendly approaches to fight bacterial pathogens is especially needed in aquaculture. We previously showed that exogenous malate reprograms zebrafish's metabolome potentiate zebrafish survival against Vibrio alginolyticus infection. However, the underlying mechanism unknown. Here, we use GC-MS based metabolomics identify malate-triggered metabolic shift. An activated TCA cycle elevated taurine are identified as key pathways most crucial biomarker reprogrammed...
Misuse and overuse of antibiotics drive the selection spread antibiotic-resistant bacteria. Although genetic mutations have been well defined for different types antibiotic resistance, ways to revert resistance are largely unexplored. Here, we adopted a proteomics approach investigate mechanism underlying ciprofloxacin in Edwardsiella tarda, representative pathogen that infects both economic animal species human beings. By comparing protein expression profiles ciprofloxacin-sensitive...
The ability of osmoregulation is crucial to marine pathogens that always face the change osmotic pressure when they shift between natural water-bodies and hosts. Previous studies indicated expressional patterns outer membrane proteins (OMPs) changed Gram-negative bacteria were transferred in different environments. In present study, proteomic methodologies used investigate pattern OMPs Vibrio alginolyticus, a universal pathogen, at Na(+) concentrations. OmpW, OmpV, Omp TolC determined be...
An unknown protein reacted with anti-human IgA, namely, IgA-like protein, has been reported in shrimp, but information regarding its identification is not available. In the present study, an affinity proteomic strategy was applied to identify of shrimp Litopenaeus vannamei. The 75 kDa isolated and confirmed by chromatography Western blotting goat respectively, then identified as hemocyanin, a member IgSF, mass spectrometry. Moreover, our results showed that human IgA L. vannamei hemocyanin...