A5033431615- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Invertebrate Immune Response Mechanisms
- Cytomegalovirus and herpesvirus research
- Seaweed-derived Bioactive Compounds
- Protein Structure and Dynamics
- interferon and immune responses
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Medicinal Plants and Bioactive Compounds
- Neurobiology and Insect Physiology Research
- Polysaccharides and Plant Cell Walls
- Viral gastroenteritis research and epidemiology
- Toxoplasma gondii Research Studies
- Marine Sponges and Natural Products
- Monoclonal and Polyclonal Antibodies Research
- Virology and Viral Diseases
- Salmonella and Campylobacter epidemiology
- Animal Virus Infections Studies
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- Nuclear Structure and Function
- Bacterial biofilms and quorum sensing
- Electromagnetic Fields and Biological Effects
Zhejiang Hospital
2017-2025
Dongguan People’s Hospital
2023
Institute of Archaeology
2020
Hangzhou Xixi hospital
2020
Zhejiang Provincial People's Hospital
2018
Xiamen University
2003-2015
Zhejiang University
2013-2015
Shaoxing University
2015
First Affiliated Hospital Zhejiang University
2013
Central China Normal University
2012
Abstract: The aim of the current study was to test hypothesis that forest bathing would be beneficial for elderly patients with chronic heart failure (CHF) as an adjunctive therapy. Two groups participants CHF were simultaneously sent or urban control area a four-day trip, respectively. Subjects exposed site showed significant reduction brain natriuretic peptide (BNP) in comparison city group and their own baseline levels. values cardiovascular disease related pathological factors, including...
The elucidation of the molecular details antibiotic resistance will lead to improvements in extending efficacy current antimicrobials. In study, proteomic methodologies were applied characterize functional outer membrane proteins (Omps) E. coli K-12 responded tetracycline and ampicillin for understanding universal pathways that form barriers antimicrobial agents. For this purpose, expressional proteome was characterized identified with use 2-DE MALDI-TOF/MS methods. Then, differential Omps...
Neurite dystrophy is a pathologic hallmark of Alzheimer's disease (AD). However, drug discovery targeting neurite protection in AD remains largely unexplored.Aβ-induced and mitochondrial damage assays were used to evaluate Aβ toxicity the neuroprotective efficacy natural compound salidroside (SAL). The 5×FAD transgenic mouse model was study function SAL. To verify direct target SAL, we surface plasmon resonance cellular thermal shift analyze drug-protein interaction.SAL ameliorates...
The ability of osmoregulation is crucial to marine pathogens that always face the change osmotic pressure when they shift between natural water-bodies and hosts. Previous studies indicated expressional patterns outer membrane proteins (OMPs) changed Gram-negative bacteria were transferred in different environments. In present study, proteomic methodologies used investigate pattern OMPs Vibrio alginolyticus, a universal pathogen, at Na(+) concentrations. OmpW, OmpV, Omp TolC determined be...
An unknown protein reacted with anti-human IgA, namely, IgA-like protein, has been reported in shrimp, but information regarding its identification is not available. In the present study, an affinity proteomic strategy was applied to identify of shrimp Litopenaeus vannamei. The 75 kDa isolated and confirmed by chromatography Western blotting goat respectively, then identified as hemocyanin, a member IgSF, mass spectrometry. Moreover, our results showed that human IgA L. vannamei hemocyanin...
Bacterium is still a major cause of many infectious diseases and global threat to human health, aquaculture, animal feeding. Prevention by vaccination the most efficient economical way fighting bacterial diseases, but one persistent challenges prevent infections disease transmissions existence multiple species, families, genera lack polyvalent vaccines against them. The information on candidate immunogens for vaccine development elusive, as well. For broad cross-protective vaccines, we have...
Nosocomial wound infections by antibiotic-resistant Pseudomonas aeruginosa strains have increasing importance in hospitals. Outer membrane proteins of the bacterium strong influence on its resistance to antibiotics. In current study, a parallel proteomic approach was applied analysis sarcosine-insoluble outer fraction P. responding ampicilin, kanamycin and tetracycline resistances. Eleven differential with 15 spots were determined then identified MALDI-TOF/MS, which four increased OprF,...
Bacterial resistance to an antibiotic may result from survival in a suddenly strong or sub-minimum inhibitory concentration of the drug. Their shared proteins responsible for should be potential targets designing new drugs inhibit growth antibiotic-resistant bacteria. In current study, comparative proteomic methodologies were used identification sharedly altered outer membrane (OM proteins) that are chloramphenical (CAP)-resistant Escherichia coli and medium with CAP treatment. Six...
Bacterium is still one of the major causes life-threatening microbial infections. The most effective way to control bacterial infections probably vaccine prevention. However, development vaccine, especially polyvalent vaccines that could be used fight against a variety serotypes and species, challenging due difficulty in identifying broad cross-protective antigens for different species pathogenic bacteria. In present study, we developed new approach identify candidates from outer membrane...
Calorie restriction (CR) is a nongenetic intervention with robust effect on delaying aging in mammals and other organisms. A mild stimulation mitochondrial biogenesis induced by CR seems to be an important action mode for its benefits. Here, we reported that component isolated from Rhodiola rosea L., salidroside, delays replicative senescence human fibroblasts, which related activating SIRT1 partly resulted inhibition miR-22. Salidroside increased the mass accompanied increment of key...
Diseases caused by microorganisms can be controlled vaccines, which require neutralizing antigens. Therefore, it is very important to identify highly efficient immunogens for immune prevention. By combining immunoproteomics and bacterial challenge after immunization, we developed a rapid method screening protected antigens of pathogenic bacteria in aquaculture. Our approach may divided into three consecutive steps. First, dominant outer membrane proteins are screened immunoproteomics....
Upregulation of outer membrane (OM) proteins was systematically investigated in response to poor iron availability the host and natural environments, but downregulation OM ill-defined this response. We utilized proteomic methodologies characterize altered sarcosine-insoluble fraction Escherichia coli K12 cultured LB medium with limitation. Notably, three novel proteins, Tsx, OmpW, OmpX, related homeostasis were identified; Tsx OmpW downregulated, OmpX upregulated. These alterations...
Membrane proteins of Gram-negative bacteria are key molecules that interface the cells with environment. Despite recent proteomic identification numerous oligomer in Escherichia coli cell envelope, protein complex E. membrane and their peripherally associated remain ill-defined. In current study, we systematically analyze subproteome envelope enriched sarcosine-insoluble fraction (SIF) sarcosine-soluble (SSF) by using methodologies. One hundred four out 184 spots on 2D electrophoresis gels...
Cross-species transmissions of swine influenza viruses (SIVs) raise great public health concerns. In this study, subcellular proteomic profiles human A549 cells inoculated with H3N2 subtype SIV were used to characterize dynamic cellular responses infection. By 2DE and MS, 27 differentially expressed (13 upregulated, 14 downregulated) cytoplasmic proteins 20 7 nuclear identified. Gene ontology analysis suggested that these mainly involved in cell death, stress response, lipid metabolism,...