A5084293545- Hepatitis B Virus Studies
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Hepatitis C virus research
- Cell Image Analysis Techniques
- Respiratory viral infections research
- Virus-based gene therapy research
- Liver Disease Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Medical Image Segmentation Techniques
- Cancer Immunotherapy and Biomarkers
- Cardiac Arrest and Resuscitation
- Viral gastroenteritis research and epidemiology
- Acute Kidney Injury Research
- Respiratory Support and Mechanisms
- Pulmonary Hypertension Research and Treatments
- Tracheal and airway disorders
- Digital Image Processing Techniques
- Advanced Proteomics Techniques and Applications
- Traditional Chinese Medicine Analysis
- Plant Virus Research Studies
- Plant Pathogenic Bacteria Studies
First Affiliated Hospital of Guangzhou Medical University
2016-2025
Guangzhou Medical University
2016-2025
State Key Laboratory of Respiratory Disease
2016-2025
Hong Kong University of Science and Technology
2013-2024
University of Hong Kong
2013-2024
Zhejiang A & F University
2024
National Clinical Research
2020
Hangzhou Normal University
2020
Xinxiang Medical University
2015
Tohoku University
2013
The association between lung microbiome and pulmonary hypertension (PH) remain unknown. This study aims to define the airway mycobiome signature its potential correlation with clinical parameters of PH. Overall, 244 patients PH 120 healthy controls (CON) were recruited from three independent centers. group was divided into subgroups not using antibiotics or corticosteroids (non-ANT/CORT), those ANT, CORT, ANT + CORT within 1 month, classification (Groups 1, 3, 4), World Health Organization...
Abstract Magnesium‐based metallic glasses (MMGs) show intriguing potentials for application as implantable biomaterials owing to their disordered atomic structure, good biodegradability, low elastic modulus, high strength, and large elasticity. However, despite of all these advantages, brittleness is Achilles’ heel, which severely limits biomedical materials. In the current study, a significantly improved ductility MMGs under bending tensile loading through minor alloying with rare‐earth...
Bacterium is still a major cause of many infectious diseases and global threat to human health, aquaculture, animal feeding. Prevention by vaccination the most efficient economical way fighting bacterial diseases, but one persistent challenges prevent infections disease transmissions existence multiple species, families, genera lack polyvalent vaccines against them. The information on candidate immunogens for vaccine development elusive, as well. For broad cross-protective vaccines, we have...
T lymphocytes, which recognize antigen peptides through specific cell receptors (TCRs), play an important role in the human adaptive immune response. TCR diversity is closely associated with host response and cancer prognosis. Although tumor-infiltrating lymphocytes have implications for tumor prognosis, few studies performed a detailed characterization of both non-tumor tissues hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC). Here, we high-throughput sequencing TCRβ chain...
Abstract Background The clinical correlates, prognosis and determinants of acute kidney injury (AKI) in patients with coronavirus disease 2019 (Covid-19) remain largely unclear. Methods We retrospectively reviewed medical records all adult laboratory-confirmed Covid-19 who were admitted to the intensive care unit (ICU) between January 23rd 2020 April 6th at Wuhan JinYinTan Hospital First Affiliated Guangzhou Medical University. Results Among 210 patients, 131 males (62.4%). median Age was 64...
During 2018–2019, a severe human adenovirus (HAdV) infection outbreak occurred in southern China. Here, we screened 18 respiratory pathogens 1704 children (≤ 14 years old) hospitalized with acute illness Guangzhou, China, 2019. In total, 151 patients had positive HAdV test results; 34.4% (52/151) of them exhibited illness. throughout the year, peak summer. The median patient age was 3.0 (interquartile range: 1.1–5.0) years. Patients increases 12 clinical indexes (P ≤ 0.019) and decreases...
We introduce an iterative thresholding algorithm for the segmentation of cells from noisy cell images. The image, which is initially a constant, changes iteratively with both previous and image local activity. Experimental results synthesized real images are provided to demonstrate performance algorithm.
Background: The long-term efficacy of the Dumon stent in treatment benign airway stenosis is unclear. Objective: objective this study was to evaluate and safety patients with stenosis. Methods: We retrospectively reviewed who were treated a at First Affiliated Hospital Guangzhou Medical University between March 2014 October 2021. included successful removal silicone stents after implantation. clinical data information on bronchoscopic interventional procedures related complications collected...
Salt stress has been identified as a vital limiting factor affecting rice output across the world. In rice, salt tolerance nature is complicated, since it dependent on different components and lowly heritable. Consequently, key method to breed salt-tolerant varieties for improving upon stress. To investigate genetic foundation of seedlings (Oryza sativa L.), bulked segregant analysis coupled with whole-genome sequencing (BSA-seq) was performed in QTL mapping huge F2 population including...
The tumor microenvironment in hepatocellular carcinoma (HCC) is complicated. Tumor-infiltrating T and B cells play a pivotal role anti-tumor immunity. cell receptor (TCR) (BCR) features may reflect the disease-associated antigen response.By combining bulk TCR/BCR-sequencing, RNA-sequencing, whole exome-sequencing, human leukocyte antigen-sequencing, we examined immune repertoire (IR) of adjacent non-tumor tissues obtained from 64 HCC patients.High IR heterogeneity with weak similarity was...
This study presents a convenient and cost-effective technology for fabricating flexible thin-film, integrative whole-Teflon microflu-idic chips. By employing micro embossing techniques, we created micropatterns on thin FEP film (~50 µm), which were then sealed with another flat (~200 µm) to form microchannels. approach enables rapid prototyping of lab-on-a-film microchips using minimal equipment at low cost. Our microfluidic chip stands out due two key factors: one is inherit-ed from the...
ABSTRACT Although the Omicron variant has been associated with greater transmissibility and tropism of upper respiratory tract, clinical pathogenic features patients infected during an outbreak in China have unclear. Adults COVID-19 were retrospectively enrolled from seven medical centers Guangzhou, China, information specimens ( BALF, sputum, throat swabs) participants collected. Conventional detection methods, metagenomics next-generation sequencing (mNGS), other methods used to detect...
Summary An interactive semi‐automatic procedure for extraction of cell contours from light microscope images is presented. The user required to specify four contour points and the algorithm determines rest automatically. exploits fact that have lower grey level than their immediate surrounding are usually very similar in shape some piece‐wise ellipses. A cost function defined detect incorporating both elliptical local image intensities. fast, reliable well suited routine applications.
The Zika virus (ZIKV) is a mosquito-borne flavivirus that causes neonatal abnormalities and other disorders. Antibodies to the ZIKV envelope (E) protein can block infection. In this study, next-generation sequencing (NGS) of immunoglobulin heavy chain (IgH) mRNA transcripts was combined with single-cell PCR cloning E-binding monoclonal antibodies for analysing antibody response in patient from early stages infection more than one year after clearance virus. patient's IgH repertoire 14 64...
Manual measurement of endobronchial optical coherence tomography (EB-OCT) images means a heavy workload in the clinical practice, which can also introduce bias if subjective opinions doctors are involved.We aim to develop convolutional neural network (CNN)-based EB-OCT image analysis algorithm automatically identify and measure parameters airway morphology.The ResUNet, MultiResUNet, Siamese were used for analyzing inner area (Ai), wall (Aw), percentage (Aw%), bifurcate segmentation obtained...
Abstract Background Airway basal stem cells (ABSCs) have self-renewal and differentiation abilities. Although an abnormal mechanical environment related to chronic airway disease (CAD) can cause ABSC dysfunction, it remains unclear how stretch regulates the behavior structure of ABSCs. Here, we explored effect on primary human Methods Primary ABSCs were isolated from healthy volunteers. A Flexcell FX-5000 Tension system was used mimic pathological conditions patients with CAD. stretched for...
Long-term treatment with nucleos(t)ide analogues (NUCs) can improve the antiviral T cell response in chronic hepatitis B (CHB) patients. Whether and to what extent is improved by NUCs early stage leading e antigen (HBeAg) seroconversion remain be clarified. A total of 22 CHB patients undergoing 2-year telbivudine-based therapy were enrolled, including 10 exhibiting a complete (CR) 12 non-complete (NCR) according HBeAg at week 52. Peripheral CD4+ CD8+ cells sorted baseline, weeks 24. The...
Laribacter hongkongensis is a facultative anaerobic, non-fermentative, Gram-negative bacillus associated with community-acquired gastroenteritis and traveller's diarrhoea. No clinical MDR L. isolate has been reported yet. We performed WGS (PacBio Illumina) on strain HLGZ1 an phenotype. was resistant to eight classes of commonly used antibiotics. Its complete genome single circular chromosome 3 424 272 bp G + C content 62.29%. In comparison the reference HLHK9, had higher abundance genes DNA...
Abstract Phosphoglucomutase (PGM) is a key enzyme in glycolysis and gluconeogenesis, regulating both glycogen trehalose metabolism insects. In this study, we explored the potential function of phosphoglucomutase using RNA interference technology Nilaparvata lugens , brown planthopper. PGM1 PGM2 were found highly expressed midgut planthoppers, with different expression levels instar nymphs. The glycogen, glucose, also significantly increased after planthoppers injected dsRNA targeting...
Un-differentiated (UD) and well-differentiated (WD) normal human primary bronchial/tracheal epithelial cells are important respiratory cell models. Mature, WD which can be derived by culturing UD at an air-liquid interface represent a good surrogate for in vivo airway epithelium. The overall protein profile of is poorly understood; therefore, the current study evaluated proteomic characteristics using label-free LC-MS/MS LC-PRM/MS. A total 3,579 proteins were identified cells. Of these, 198...
An algorithm for segmentation and thickness measurement of the glomerular basement membranes (GBM) in electron microscopy kidney images is presented. Differences intensities variations between GBM other components image are employed. Regions extreme such as black area blood cells white areas urinary spaces pre-excluded. Areas sharp edges either at borders or unrelated to regions. These non-GBM edges, along with pre-excluded regions, used barriers limiting size fitting circles centered a...