A5065241965- Biosensors and Analytical Detection
- Reproductive tract infections research
- Advanced Biosensing Techniques and Applications
- Respiratory viral infections research
- Electrowetting and Microfluidic Technologies
- Microfluidic and Bio-sensing Technologies
- Influenza Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- Brucella: diagnosis, epidemiology, treatment
- Antifungal resistance and susceptibility
- Bacterial Identification and Susceptibility Testing
- Photoacoustic and Ultrasonic Imaging
- Microfluidic and Capillary Electrophoresis Applications
- Cervical Cancer and HPV Research
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Extracellular vesicles in disease
- Syphilis Diagnosis and Treatment
- Nanopore and Nanochannel Transport Studies
- Microbial infections and disease research
Tsinghua University
2022-2025
Recombinase polymerase amplification (RPA) is a useful pathogen identification method. Several label-free detection methods for RPA amplicons have been developed in recent years. However, these still lack sensitivity, specificity, efficiency, or simplicity. In this study, we propose rapid, highly sensitive, and assay system based on solid-phase self-interference chip (SiSA-chip) hyperspectral interferometry. The SiSA-chips amplify capture the chips, rather than irrelevant such as primer...
species are the most common cause of fungal infections around world, associated with superficial and even deep-seated infections. In clinical practice, there is great significance in identifying different
Pathogen, prevalent in both natural and human environments, cause approximately 4.95 million deaths annually, ranking them among the top contributors to global mortality. Traditional pathogen detection methods, reliant on microscopy cultivation, are slow labor-intensive often produce subjective results. While nucleic acid amplification techniques such as polymerase chain reaction offer genetic accuracy, they necessitate costly laboratory equipment skilled personnel. Consequently, isothermal...
Rapid identification of Candida species is significant for the diagnosis vulvovaginal candidiasis (VVC). An integrated and multi-target system rapid, high-specificity, high-sensitivity detection four was developed. The consists a rapid sample processing cassette nucleic acid analysis device. could process to release acids in 15 min. released were analyzed by device as fast within 30 min, using loop-mediated isothermal amplification method. be simultaneously identified, with each reaction...
Cervical cancer is a significant global health issue primarily caused by high-risk types of human papillomavirus (HPV). Recent studies have reported an association between Trichomonas vaginalis (T. vaginalis) infections and HPV infections, highlighting the importance simultaneously detecting these pathogens for effective cervical risk management. However, current methods both T. are limited. In this study, we present novel approach using microfluidic-chip-based system with loop-mediated...
Purpose: The aim of this study is to establish a loop-mediated isothermal amplification (LAMP) method for the rapid detection vulvovaginal candidiasis (VVC). Methods: We developed and validated detecting most common Candida species associated with VVC, including C. albicans, N. glabratus, tropicalis, parapsilosis. evaluated specificity, sensitivity, positive predictive value (PPV), negative (NPV), Kappa LAMP detect different species, using conventional culture internal transcribed spacer...
Influenza A virus outbreaks pose a significant burden to global health. However, detecting and subtyping the is challenging due its high mutation rate, it difficult distinguish viable that can cause infection from clinical samples. In this study, we developed novel PMAxx-LAMP assay for simultaneous detection of non-viable influenza virus, as well differentiation between H1N1 H3N2 subtypes. Our LAMP primers displayed limit E2 copies/μL both subtypes, specificity, reproducibility, good...
Influenza A virus outbreaks pose a significant burden to global health. However, detecting and subtyping the is challenging due its high mutation rate, it difficult distinguish viable viruses that cause infection from clinical samples. Here novel PMAxx-LAMP assay for classification of non-viable together with differentiation between H1N1 H3N2 subtypes. Our loop-mediated isothermal amplification (LAMP) primers displayed limits detection 102 copies/μL both subtypes, specificity,...
Nucleic acid detection is widely used in life science and clinical medical diagnosis. Innovative methods platform research to improve its key performances are of great significance ensure population health, promote precision technology, even social stability development. Most the existing nucleic technologies utilized PCR as amplification method, relying on professional complex scientific instruments thus time-consuming laborious. Fortunately, RPA offers a feasible alternative. It has...
As a biomarker for the diagnosis and treatment monitoring of various diseases, exosomes widely exist in body fluids such as blood, urine saliva. However, its small particle size low content are difficult to enrich. Therefore, fast, high-purity enrichment method, high-sensitivity, high-resolution, detection limit method particularly important. We developed an automated fully integrated system exosomes. Using magnetic beads modified with anionic polymers capture exosomes, adjust pH exosome...