A5042384881- Extracellular vesicles in disease
- Hemoglobinopathies and Related Disorders
- Advanced Proteomics Techniques and Applications
- Iron Metabolism and Disorders
- Biosensors and Analytical Detection
- Clusterin in disease pathology
- Mass Spectrometry Techniques and Applications
- Glycosylation and Glycoproteins Research
- Bioactive natural compounds
- Tannin, Tannase and Anticancer Activities
- Peptidase Inhibition and Analysis
- Natural product bioactivities and synthesis
- Arsenic contamination and mitigation
- Erythrocyte Function and Pathophysiology
- Curcumin's Biomedical Applications
- Periodontal Regeneration and Treatments
- Flavonoids in Medical Research
- Retinoids in leukemia and cellular processes
- Chemical Analysis and Environmental Impact
- Thyroid Cancer Diagnosis and Treatment
- Bioactive Compounds and Antitumor Agents
- Vitamin D Research Studies
- Pharmacological Effects and Toxicity Studies
- Galectins and Cancer Biology
- Bone and Dental Protein Studies
Chulabhorn Research Institute
2016-2025
Prince of Songkla University
2019-2022
Mahidol University
2009-2012
Siriraj Hospital
2012
Due to the invasive procedure associated with Pap smears for diagnosing cervical cancer and conservative culture of developing countries, identifying less biomarkers is great interest. Quantitative label‑free mass spectrometry was performed identify potential in urine samples patients cancer. This technique used study differential expression urinary proteomes between normal individuals patients. The alterations levels were analyzed by Progenesis software results revealed that 60 proteins...
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The northeastern region of Thailand is well known to have a high incidence and mortality cholangiocarcinoma (CCA). Protein phosphorylation status has been reported reflect key determinant cellular physiology, but identification phosphoproteins can be problem due the presence phosphatase. Exosomes are stable toward circulating proteases other enzymes in human blood recognized before onset cancer progression. Here an vitro metastatic model isogenic CCA cells used provide insight into levels...
Cervical cancer is one of the most common causes cancer-associated mortality in females worldwide. Serum biomarkers are important tools for diagnosis, disease staging, monitoring treatment and detecting recurrence different types cancer. However, only a small number established have been used clinical diagnosis cervical Therefore, identification minimally invasive, sensitive highly specific detection may improve outcomes. In present pilot study, changes disease‑relevant proteins 31 patients...
A promising nutraceutical, apigenin, was recently revealed to exhibit biological activity in inhibiting several types of cancer. The effects apigenin on the growth inhibition and apoptosis cholangiocarcinoma HuCCA‑1 cell line were investigated. Protein alterations subsequent treatment studied using a proteomic approach. values 20, 50 90% (IC20, IC50 IC90) determined by MTT viability assay. Apoptotic death detected two different methods, flow cytometric analysis (Muse Cell Analyzer) DNA...
The analysis of urinary proteome might reveal biomarkers clinical value. However, current methods urine preparation for down-stream proteomic are complicated, time-consuming, and/or expensive. This study aims to develop a robust, simple, inexpensive and readily accessible method facilitate workflow.Syringe-push membrane absorption (SPMA) was successfully developed by combination 5-ml medical syringe protein-absorbable membrane. Comparing three membranes i.e., nitrocellulose, polyvinylidene...
Typically, biological systems are protected from the toxic effect of free radicals by antioxidant defense. Extracts orchids have been reported to show high levels exogenous activity including Bulbophyllum but so far, there no reports on enzymes. Therefore, differences in protein expression leaves and pseudobulbs morphologlorum Kraenzl Dendrobium Sonia Earsakul were studied using two-dimensional gel electrophoresis mass spectrometry (LC/MS/MS). Interestingly, largest group these stress...
Summary Splenectomised β‐thalassaemia/haemoglobin E (HbE) patients have increased levels of circulating microparticles or medium extra‐cellular vesicles (mEVs). The splenectomised mEVs play important roles in thromboembolic complications since they can induce platelet activation and endothelial cell dysfunction. However, a comprehensive understanding the mechanism mEV generation thalassaemia disease has still not been reached. Thalassaemic are hypothesised to be generated from cellular...
Background: This study aimed to identify differentially expressed proteins in the serum of advanced non-small cell lung cancer (NSCLC) patients responding carboplatin (CAR) plus paclitaxel (PTX) chemotherapy compared non-responders. Materials and Methods: Serum from 8 responders 6 non-responders was subjected proteomic analysis by label-free liquid chromatography tandem mass spectrometry validated western blotting. CAR/PTX-resistant human H1792 A549 cells were used for evaluating gene...
Cholangiocarcinoma (CCA), derived from the bile duct, occurs with a relatively high incidence in Northeast Thailand. Early diagnosis is still hampered by lack of sufficient biomarkers. In recent years, biomarker discovery using secretomes has provided interesting results, including our studies on CCA secretomes, especially three-dimensional cell cultures. Thus, cells cultured hollow fiber bioreactor (HFB) 20 kDa molecular weight cut-off (MWCO) yielded higher quality and quantity than those...
The incidence rate of hepatocellular carcinoma (HCC) remains high in numerous countries, including Thailand. There are different lines HCC treatment; however, various side effects and the resistance cancer cells during treatment remain issues. At present, traditionally used herb plants have been widely as alternatives to therapy. Derris scandens is a Thai traditional which commonly found Thailand medicine for diseases. cytotoxicity D. ethanolic extract on cell line (HCC‑S102) was determined...
Objective: Iron overload and oxidative stress are the major causes of serious complications mortality in thalassemic patients. Our previous work supports synergistic effects antioxidant cocktails (curcuminoids or vitamin E, N-acetylcysteine, deferiprone) treatment β-thalassemia/Hb E This further 2-DE-based proteomic study aimed to identify plasma proteins that expressed differentially response cocktails.Methods: Frozen samples ten normal subjects patients at three-time points (baseline,...
Prefractionation is a prerequisite step for deep plasma proteomics. Highly abundant proteins, particularly human serum albumin (HSA) and immunoglobulin G (IgG), typically interfere with investigation of proteins lower abundance. A relatively simple preparation method based on high temperature can precipitate thermolabile providing strategic window to access the thermostable subproteome. This study aimed optimize thermal treatment as reliable prefractionation compare it two commercial kits,...
Cholangiocarcinoma (CCA) is a highly lethal disease because most patients are asymptomatic until they progress to advanced stages. Current CCA diagnosis relies on clinical imaging tests and tissue biopsy, while specific biomarkers still lacking. This study employed translational proteomic approach for the discovery, validation, development of multiplex biomarker assay. In discovery phase, label-free quantitation was performed nine pooled plasma specimens derived from patients, controls (DC),...
Protein O-linked-N-acetylglucosaminylation (O-GlcNAcylation) is a dynamic post-translational modification process that plays an essential role in biological activities. Growing evidence indicates aberration of O-GlcNAcylation associated with various diseases, e.g. diabetes, neurological and cancers. However, the mechanistic studies are lagging behind other modifications due to its extremely low abundance, limited analytical tools, specificity. Herein, diagonal strong cation exchange...
Ethidium bromide, which is commonly applied in molecular biological applications, a mutagenic agent demanding proper treatment. In the present study, magnetic fraction of fly ash, prepared from ASTM class F material, was used as catalyst for heterogeneous Fenton process degrading ethidium bromide aqueous solution. The dosage catalyst, initial pH solution, and concentration hydrogen peroxide significantly influenced removal rate. Under optimum conditions (2 mmol/L 100 g/L ash at 3), an...
Thalassemia is a group of genetic disorders resulting from different mutations in the globin gene clusters and leading to an imbalance synthesis. Unpaired chains are less stable susceptible oxidation. Patients with beta‐thalassemia/HbE (β‐thal/HbE) prone increase oxidative stress as indicated by increased lipid peroxidation protein Curcumin, strong antioxidant iron chelator, has been shown decrease β‐thal/HbE patients. In this study, effect curcumin on plasma expression was investigated...