Animal studies have implicated an important role of nitric oxide (NO) in the regulation blood pressure, renal hemodynamics, and excretion sodium. NG-monomethyl-L-arginine (L-NMMA) is a specific, competitive inhibitor NO synthesis interfering with synthase. The purpose present study was to investigate effect L-NMMA on plasma flow (RPF), glomerular filtration rate (GFR), urinary sodium (UNa), fractional (FENa), lithium (FELi), mean arterial pressure (MAP), heart (HR) healthy humans. In...

The effect of strict metabolic control for 1 yr on renal function and retinal morphology was estimated in 24 insulin-dependent diabetic individuals (age 29 ± 8 yr, diabetes duration 10 6 yr) with Albustix negative urine minimal or no background retinopathy before the study. They were randomized to conventional insulin treatment (CIT) continuous subcutaneous infusion (CSII) a portable pump. During CSII status significantly improved glomerular filtration rates (GFR) found decline (from 130 18...

Singlet molecular oxygen, O2(a1Deltag), can be efficiently produced in a photosensitized process using either one- or two-photon irradiation. The aromatic ketone 1-phenalenone (PN) is an established one-photon singlet oxygen sensitizer with many desirable attributes for use as standard. In the present work, photophysical properties of two other ketones, pyrene-1,6-dione (PD) and benzo[cd]pyren-5-one (BP), are reported compared to those PN. Both PD BP sensitize production near unit quantum...

Animal studies have indicated that increased nitric oxide (NO) synthesis plays a significant role in the renal adaptation to sodium intake. To investigate of NO during intake humans, we studied effect acute, systemic injection N G -monomethyl-l-arginine (l-NMMA) on hemodynamics [glomerular filtration rate and plasma flow (GFR RPF, respectively)], urinary excretion (FE Na ), [mean arterial blood pressure heart (MAP HR)], levels several vasoactive hormones 12 healthy subjects high (250...

Substituent-dependent features and properties of the sensitizer play an important role in photosensitized production singlet oxygen, O2(a1Δg). In this work, we systematically examine effect molecular changes on efficiency oxygen using, as sensitizer, oligophenylene-vinylene derivatives designed to optimally absorb light a nonlinear two-photon process. We demonstrate that one cannot always rely rule-of-thumb guidelines when attempting construct efficient sensitizers. Rather, consequence...

Plasma renin activity (PRA) and plasma concentrations of angiotensin II (AngII), atrial natriuretic peptide (ANP) arginine vasopressin (AVP) were determined in the abdominal aorta renal veins before 1 h after peroral ingestion captopril 25 mg 29 patients with arterial hypertension unilateral artery stenosis or occlusion, order to study effect ACE inhibition on single-kidney extraction ratio (ER) PRA, AngII, ANP, AVP, vein (RVRR). PRA was increased, AngII ANP reduced, AVP unchanged captopril....

Two-photon excitation spectra have been recorded over the large spectral range of 540–1000 nm for five phenylene–vinylene oligomers that differ in length conjugated π system. The significant changes observed two-photon and absorption cross sections as a function this systematic change chromophore are discussed light (1) corresponding one-photon (2) high-level density functional response theory calculations performed on analogues these systems. results obtained illustrate one way to exploit...

The effects of a solvent on the two-photon absorption microsolvated formaldehyde and liquid water have been studied using hybrid coupled-cluster/molecular mechanics (CC/MM) response theory. Both were considered solvated in water, where molecules described within framework molecular mechanics. Prior to CC/MM calculations, dynamics simulations performed water/formaldehyde water/water aggregates many configurations generated. By carrying out calculations individual configurations, it was...

We have investigated the acute effects of rapamycin on renal hemodynamics, water, sodium and lithium excretion rates, plasma levels angiotensin II, aldosterone, atrial natriuretic peptide vasopressin in 34 Lancaster/Yorkshire female pigs, a breed that has structure/function resembling human kidney. Three different dosages were given over 1-hr period: dose A, 0.1 mg/kg (n=8); B, 0.2 C, 0.4 P, placebo vehicle (n=10). Glomerular filtration rate (GFR) flow (RPF) measured by constant infusion...

The yield of singlet molecular oxygen, O2(a1Δg), produced in a photosensitized process can be very susceptible to environmental perturbations. In the present study, protonation photosensitizers whose chromophores contain amine functional groups is shown adversely affect oxygen yield. Specifically, for bis(amino) phenylene vinylenes dissolved both water and toluene, addition protic acid solution alters properties system that, turn, result decrease efficiency production. light previous studies...

Our purpose was to elucidate the hypothesis that paracrine-produced transforming growth factor (TGF)-beta1 regulates accumulation of extracellular matrix (ECM) in renal glomeruli, a hallmark diabetic nephropathy. To produce TGF-beta1 from juxtaglomerular apparatus mouse kidneys, we cloned Ren-1c promoter fragment (-4.100 +6 base pairs) upstream porcine (pTGF-beta1) cDNA, mutated ensure secretion biologically active TGF-beta beta1. The resulting transgenic mice had significantly more their...

Abstract Phenylephrine (10mg/ml), phentolamine (10 mg/ml), isoprenaline (0.5 mg/ml) and timolol (5 mg/ ml) were applied topically to one eye of volunteers with normal corneas, the other serving as a control. One drop each drug was given twice day for 3 or 4 days. Timol fond increase corneal thickness in both midicated control eye. A significantly greater effect noted on receiving directly. When days, slight significant decrease found medicated eye, two eyes exhibiting similar CCT change. The...

We have studied the basis set and electron correlation effects on ab initio calculations of two-photon absorption cross sections water. Various series consistent sets up to triply augmented valence pentuple zeta level as well popular 6-31G(d) been employed in combination with several coupled cluster, configuration interaction, density functional theory methods. find that it is very difficult obtain converged values for even a small molecule such Acknowledging these difficulties obtaining...

Aims To investigate the acute effects of prostacyclin (Flolan ® ) on renal haemodynamics, tubular function, plasma concentration angiotensin II (Ang II), aldosterone (Aldo), atrial natriuretic peptide (ANP), arginine vasopressin (AVP), mean arterial blood pressure (MBP), and heart rate (HR). Methods Thirteen healthy control subjects were investigated two separate occasions in a placebo controlled, randomized, dose–response study effect intravenous infusion (PGI 2 , Flolan 2, 4 8 ng kg −1 min...

A technique is described wherein substituent-dependent excited-state properties of gas-phase porphyrins can be accurately quantified. Dissociation lifetimes photoexcited porphyrin cations were measured in an electrostatic ion storage ring. From these data, upper limits for the triplet quantum yield following protonated obtained: protoporphyrin IX (0.63 ± 0.04), tetraphenylporphyrin (0.73 0.03), tetra(p-methylphenyl)porphyrin (0.75 and tetra(p-cyanophenyl)porphyrin (0.71 0.03). The values...

Albuminuria is a sensitive marker for renal dysfunction. Urinary dipstick tests are frequently used to screen urinary abnormalities in the emergency department (ED). The aim of this prospective cohort study evaluate usefulness testing as screening tool albuminuria ED setting and determine persistency identified acute setting. spot urine samples were obtained simultaneously analysis albumin-creatinine ratio (ACR). Participants with positive dipsticks protein invited second urinalysis four six...

Abstract. A 90–min intravenous infusion of the direct vasodilator sodium nitroprusside (SNP) was compared with a placebo in 32 healthy control subjects order to study acute effects SNP on renal haemodynamics, tubular function evaluated by lithium clearance technique, plasma levels atrial natriuretic peptide (ANP), angiotensin II (Ang II), aldosterone (Aldo) and arginine vasopressin (AVP) transport cGMP (T ). induced significant reduction mean arterial blood pressure (from 89.5 81.5 mmHg),...

Abstract. The sensitivity of the indirect Coombs' test was investigated using saline, albumin, or low ionic strength (LIS) in incubation phase and an time varying from 5 to 120 min. Titration 41 antibodies showed that LIS always resulted a higher almost same mean scores as other solutions after time. maximum score with than saline reached shorter period incubation, only 20–40 min even weakest antibodies. With LIS, longer 40 sudden decrease score. albumin 40–60 incubation. obtained 15–20 min,...

Abstract Twentyfour insulin dependent juvenile diabetics, with no or minimal background retinopathy, were randomly allocated to conventional therapy (CIT) continuous subcutaneous infusion (CSII) administrated by means of a portable pump. The metabolic control was significantly improved in the CSII group as compared CIT group. After one year, progression diabetic retinopathy (criterion: development more than 2 microaneurysms) observed 3 12 patients and 4 ( P > 0.05). A tendency severe None...

The effects of an ACE-inhibitor (ramipril), a calcium antagonist (felodipine) and placebo on glomerular filtration rate (GFR), urinary albumin/creatinine ratio, blood pressure (BP) vasoactive hormones were investigated in randomized, prospective, double-blind, placebo-controlled study patients with chronic glomerulonephritis hypertension, measurements at entrance after 12 24 months. In total, 33 included: 21 completed the 7 each group. GFR was measured as 51Cr-EDTA clearance...

We have investigated the acute effects of rapamycin on renal hemodynamics, water, sodium and lithium excretion rates, plasma levels angiotensin II, aldosterone, atrial natriuretic peptide vasopressin in 34 Lancaster/Yorkshire female pigs, a breed that has structure/function resembling human kidney. Three different dosages were given over 1-hr period: dose A, 0.1 mg/kg (n=8); B, 0.2 C, 0.4 P, placebo vehicle (n=10). Glomerular filtration rate (GFR) flow (RPF) measured by constant infusion...

Accessory mitral valve tissue (AMVT) is a rare congenital cardiac anomaly often diagnosed in childhood and associated with left ventricular outflow tract obstruction. We present case of an asymptomatic adult man his 60s incidental finding AMVT. A transthoracic echocardiography transesophageal initially raised suspicion AMVT, but the precise origin was established using dynamic 4-dimensional computed tomography scan, ultimately confirming diagnosis. This highlights potential value...

1. Animal studies have shown that prostaglandins are important for renal function after unilateral nephrectomy. In order to investigate the importance of in fully adapted remnant kidney healthy uninephrectomized subjects, acute effects indomethacin on haemodynamics, lithium clearance, urinary excretion rates prostaglandin E2, sodium and water, plasma levels angiotensin II, aldosterone, atrial natriuretic peptide arginine vasopressin were measured 14 subjects (median time nephrectomy 1.7...

Abstract. Objective. To examine whether the effect of atrial natriuretic peptide (ANP) on renal glomerular and tubular segmental handling sodium in patients with essential hypertension is pressure dependent. Design. Part 1 . The effects a low‐dose continuous infusion (10 ng kg −1 min ) ANP for h were compared 10 untreated hypertensives (EH) 13 normotensive control subjects (CS). 2 studied another day during preceding acute BP reduction nitroprusside (NP). results those obtained + placebo...