Inflammation and reactive oxygen species (ROS) play important roles in the pathogenesis of atherosclerosis. Resveratrol has been shown to possess anti-inflammatory antioxidative stress activities, but underlying mechanisms are not fully understood. In present study, we investigated molecular basis associated with protective effects resveratrol on tumor necrosis factor-alpha (TNF-α)-induced injury human umbilical endothelial cells (HUVECs) using a variety approaches including cell viability...

Endothelial inflammation is an important contributor to the pathology of atherosclerotic cardiovascular disease (ASCVD). Circular RNAs (circRNAs) function and role in endothelium still unknown. In our present study, we firstly identified that circ-RELL1 plays a proinflammatory ox-LDL-induced HUVECs through high-throughput circRNA microarray assays. Knockdown can reduce expression ICAM1 VCAM1 ox-LDL induced inflammation. Mechanistically, directly bound miR-6873-3p cytoplasm. Subsequently...

Abstract Background Hypertension (HT) is the most prevalent risk factor for cardiovascular disease (CVD) worldwide. Despite being a highly heritable trait, underlying mechanisms of HT remain elusive due to its complex genetic architecture. Discovering disease-associated proteins with causal evidence offers potential strategy identifying therapeutic targets HT. Methods We analyzed plasma proteome 4,657 from 7,213 European American (EA) participants in ARIC study. Genome-wide association study...

BACKGROUND:Resveratrol has been shown to possess beneficial activities including antioxidant, anti-inflammatory, and cardioprotective effects through activating a nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylase family member sirtuin-1 (SIRT1) protein. The current study was undertaken investigate the role of sirtuin members (SIRT1–SIRT7) on anti-inflammation resveratrol in endothelial cells. MATERIAL AND METHODS:Primary human umbilical vein cells (HUVECs) were pretreated...

As the incidence of heart failure (HF) increases, need for practical tools to evaluate long-term prognosis in these patients remains critical. Our study aimed develop a 48 month prediction model all-cause mortality decompensated HF using available clinical indicators.

Abstract An effective multiplex real-time polymerase chain reaction (PCR) assay for the simultaneous detection of three major pathogens, Nosema bombycis Nägeli (Microsporidia: Nosematidae), Bombyx mori nucleopolyhedrovirus (Baculoviridae: genus Alphabaculovirus ) (NPV), and densovirus (Parvoviridae: Iteravirus (DNV), in silkworms ( (Linnaeus); Lepidoptera: Bombycidae) was developed this study. Polymerase PCR tests basic local alignment search tool searches revealed that primers probes used...

Myocardial ischemia/reperfusion (I/R) injury is a serious complication of reperfusion therapy for myocardial infarction. At present, there not an effective treatment strategy available I/R. The present study aimed to investigate the effects human tissue kallikrein 1 (hTK1) and inhibitors matrix metalloproteinase (hTIMP1) gene co‑expression on I/R injury. A rat model cell with hypoxia/reoxygenation (H/R) in cardiac microvascular endothelial cells (CMVECs) were established, treated adenovirus...

Tissue kallikrein 1 (TK1) and tissue inhibitor of matrix metalloproteinase (TIMP1) are important in inhibiting vascular smooth muscle cell (VSMC) proliferation improving remodeling, respectively. It was hypothesized that a combination TK1 TIMP1 genes, mediated by an adenovirus vector could augment or act synergy to enhance the inhibitory effects. The promoter, mCMV carrying hTIMP1 cDNA subcloned into pDC316‑hTK1 construct recombinant plasmid hTK1 genes. Subsequently, double gene backbone...

AIM:To determine whether expression of the tumor suppressors p16 and Rb is altered in gastric carcinoma. METHODS:Mucosal biopsies were endoscopically obtained from patients with superficial gastritis (n = 12), atrophic 15), atypical hyperplasia 20) cancer 40).Upon obtainment, all samples immediately fixed 10% buffered formalin, embedded paraffin, sectioned serially.Protein was detected by immunohistochemistry (ABC method). RESULTS:The epithelium showed various degrees nuclear immunostaining...

Sonic hedgehog (Shh) is pivotally important in embryonic and adult blood vessel development homeostasis. However, whether Shh involved atherosclerosis plays a role endothelial apoptosis induced by oxidized low‑density lipoprotein (ox‑LDL) has not been reported. The present study used recombinant Shh‑N protein (rShh‑N) plasmid encoding the human gene (phShh) to investigate of ox‑LDL‑mediated umbilical vein cell (HUVEC) apoptosis. found that ox‑LDL was able induce HUVECs expression...

Objective: Tissue kallikrein 1 (TK1) and tissue inhibitor of matrix metalloproteinase (TIMP1) are important in inhibiting vascular smooth muscle cell (VSMC) proliferation improving remodeling, respectively. It was hypothesized that a combination TK1 TIMP1 genes, mediated by an adenovirus vector could augment or act synergy to enhance the inhibitory effects. Design Method: The promoter, mCMV carrying hTIMP1 cDNA subcloned into pDC316—hTK1 construct recombinant plasmid hTK1 genes....

Objective: Inflammation and reactive oxygen species (ROS) play important roles in the pathogenesis of atherosclerosis. Resveratrol has been shown to possess anti-inflammatory antioxidative stress activities, but underlying mechanisms are not fully understood. Design Method: In present study, we investigated molecular basis associated with protective effects resveratrol on tumor necrosis factor-alpha (TNF-α)-induced injury human umbilical endothelial cells (HUVECs) using a variety approaches...