A5112329363- Arsenic contamination and mitigation
- Chromium effects and bioremediation
- Microbial Community Ecology and Physiology
- Advanced biosensing and bioanalysis techniques
- Heavy metals in environment
- Fluoride Effects and Removal
- Genomics and Phylogenetic Studies
- Selenium in Biological Systems
- Mine drainage and remediation techniques
- Plant Virus Research Studies
- Curcumin's Biomedical Applications
- Microbial Metabolites in Food Biotechnology
- Environmental Toxicology and Ecotoxicology
- Probiotics and Fermented Foods
- Obesity, Physical Activity, Diet
- Bacterial biofilms and quorum sensing
- Olfactory and Sensory Function Studies
- Microbial Fuel Cells and Bioremediation
- Pharmaceutical and Antibiotic Environmental Impacts
- Biochemical Analysis and Sensing Techniques
- bioluminescence and chemiluminescence research
- Folate and B Vitamins Research
- Plant Pathogenic Bacteria Studies
- Pesticide and Herbicide Environmental Studies
- Hydrogen's biological and therapeutic effects
Nanjing Agricultural University
2012-2025
Children's Hospital of Zhejiang University
2024
Zhejiang Sci-Tech University
2020
Jishou University
2009
Abstract Microbial arsenic (As) methylation and demethylation are important components of the As biogeochemical cycle. Arsenic is enhanced under flooded conditions in paddy soils, producing mainly phytotoxic dimethylarsenate (DMAs) that can cause rice straighthead disease, a physiological disorder occurring widely some growing regions. The key microbial groups responsible for soils unknown. Three were incubated conditions. DMAs initially accumulated soil porewater, followed by rapid...
Arsenic (As) bioavailability to rice plants is elevated in flooded paddy soils due reductive mobilization of arsenite [As(III)]. However, some microorganisms are able mediate anaerobic As(III) oxidation by coupling nitrate reduction, thus attenuating As mobility. In this study, we investigated the impact additions on species dynamics porewater four As-contaminated soils. The effects microbial community structure and abundance diversity oxidase (aioA) genes were quantified using 16S rRNA...
Microbial arsenic (As) methylation and volatilization are important processes controlling the As biogeochemical cycle in paddy soils. To further understand these processes, we isolated a novel bacterial strain, SM-1, from an As-contaminated soil. SM-1 showed strong abilities, converting almost all arsenite (10 μM) to dimethylarsenate trimethylarsenic oxide medium trimethylarsine gas into headspace within 24 h, with accounting for nearly half of total As. On basis 16S rRNA sequence, strain...
Organic manures may contain high levels of arsenic (As) due to the use As-containing growth-promoting substances in animal feed. To develop a bioremediation strategy remove As from organic waste, Bacillus subtilis 168, bacterial strain which can grow at temperature but is unable methylate and volatilize As, was genetically engineered express arsenite S-adenosylmethionine methyltransferase gene (CmarsM) thermophilic alga Cyanidioschyzon merolae. The B. 168 converted most inorganic medium into...
Summary Arsenic (As) biomethylation is an important component of the As biogeochemical cycle that can influence toxicity and mobility in environment. Biomethylation catalyzed by enzyme arsenite (As[III]) S ‐adenosylmethionine methyltransferase (ArsM). To date, all identified ArsM orthologs with As(III) methylation activities have four conserved cysteine residues, which are thought to be essential for methylation. Here, we isolated As(III)‐methylating bacterium, Bacillus sp. CX‐1, a gene...
Abstract Metabolic cross-feeding is a pervasive microbial interaction type that affects community stability and functioning directs carbon energy flows. The mechanisms underlie these interactions their association with metal/metalloid biogeochemistry, however, remain poorly understood. Here, we identified two soil bacteria, Bacillus sp. BP-3 Delftia DT-2, engage in two-tiered mutualism. Strain has low utilization ability of pyruvic acid while strain DT-2 lacks hexokinase, phosphotransferase...
Impact statement Arsenic is the most common toxic metalloid in environment. Nearly all organisms have genes for arsenic detoxification. detoxification are frequently organized chromosomal or plasmid‐encoded resistance ( ars ) operons, which commonly regulated by members of ArsR transcriptional repressors. To date, three As(III)‐responsive ArsRs with different As(III) binding sites been identified. Here, we identify a new type repressor that has an atypical site and controls transcription...
Arsenic (As) biovolatilization is an important component of the global As biogeochemical cycle. Soils can emit various methylarsine gases, but underlying microbial processes remain unclear. Here, we show that addition molybdate (Mo), inhibitor sulfate-reducing bacteria, greatly enhanced dimethylarsine evolution from dimethylarsenate [DMAs(V)] added to two paddy soils. Molybdate significantly affected community structure. The aerobic enrichment cultures both soils volatilized substantial...
Trivalent arsenicals such as arsenite (As
Microbial arsenic (As) methylation is an important process of As biogeochemistry. Only a few As-methylating microorganisms have been isolated from paddy soil, hindering the mechanistic understanding involved. We 54 anaerobic and 32 aerobic bacteria soil with high potential. Among 86 isolates, 14 anaerobes, including 7 sulfate-reducing (SRB), but none aerobes were able to methylate arsenite [As(III)] or monomethylarsenite [MMA(III)] both, suggesting that ability much more prevalent in...
A novel bacterium with strong arsenic (As) methylation and volatilization abilities, designated strain SM-1T, was isolated from an As-contaminated paddy soil. SM-1T is strictly aerobic, rod-shaped, non-motile, Gram-negative orange-coloured. Phylogenetic analysis revealed that showed low 16S rRNA gene sequence similarities (≤88 %) to members of established genera in the family Cytophagaceae. Growth this observed at 15-45 °C (optimum, 37 °C), pH 6.0-8.0 7.0) 0-0.5 % (w/v) NaCl. The major...
Roxarsone (ROX) is an organoarsenic feed additive that has been widely used in poultry industries for growth promotion, coccidiosis control, and meat pigmentation improvement more than 70 years. Most ROX excreted the litter dispersed into environment, where it transformed by microbes different arsenic-containing compounds. A major product of transformation N -acetyl-4-hydroxy- m -arsanilic acid (N-AHPAA), which also as a clinical drug treating refractory bacterial vaginosis. Here, we report...
Rice is a major dietary source of inorganic arsenic (iAs), highly toxic arsenical that accumulates in rice and poses health risks to rice-based populations. However, the availability detection methods for iAs grains limited. In this study, we developed novel approach utilizing natural bacterial biosensor, Escherichia coli AW3110 (pBB-ArarsR-mCherry), conjunction with amylase hydrolysis efficient extraction, enabling high-throughput quantitative grains. The biosensor exhibits high specificity...
Arsenic is a ubiquitous environmental toxin. Some microbes detoxify inorganic arsenic through biomethylation, generating relatively nontoxic pentavalent methylated arsenicals, such as methylarsenate.
Methylarsenite (MAs(III)), a product of arsenic biomethylation or bioreduction methylarsenate (MAs(V)), has been proposed as primitive antibiotic. However, the antibacterial property and bactericidal mechanism MAs(III) remain largely unclear. In this study, we found that is highly toxic to 14 strains bacteria, especially against 9 Gram-positive bacteria with half maximal inhibitory concentration (IC50) in sub micromolar range for Staphyloccocus aureus, Microbacterium sp., Pseudarthrobacter...
Steviol glycosides, mostly extracted from Stevia rebaudiana Bertoni, are non-caloric, safe natural sweeteners. Steviosides make up more than 50% of total steviol glycosides but possess a bitter aftertaste, which limits their application as food additives. Trace amounts rubusosides acceptable sweeteners, supply is limited. In this study, we screened an endophytic bacteria (Lelliottia LST-1) that can specifically convert stevioside into rubusoside and reach conversion rate 75.4% after response...
Abstract The pathogenic bacteria of ginger plantlets were isolated and identified. Pathogenic strains isolated, purified, then identify by morphology observation, physiological biochemical experiments, the 16s rDNA sequence was analyzed to verify classification pahtogen. This study shows novel is kind Gram-negative ones, which can be well root on tissue as cultivated plantlets. histopathology examination illustrates that pathogen prefer infect host plant through tuber. Moreover, the16S...