A5060896563- Barrier Structure and Function Studies
- Drug Transport and Resistance Mechanisms
- Chemokine receptors and signaling
- Immune Response and Inflammation
- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Animal testing and alternatives
- Skin Protection and Aging
- interferon and immune responses
- Effects and risks of endocrine disrupting chemicals
- Contact Dermatitis and Allergies
- Connexins and lens biology
- Drug Solubulity and Delivery Systems
- Carcinogens and Genotoxicity Assessment
- Health, Environment, Cognitive Aging
- Computational Drug Discovery Methods
- Drug-Induced Hepatotoxicity and Protection
- Pharmacogenetics and Drug Metabolism
- Gut microbiota and health
- Hedgehog Signaling Pathway Studies
- Body Contouring and Surgery
Biopredic (France)
2010-2024
The Open University
2005-2009
Establishment of a human model the blood-brain barrier has proven to be difficult goal. To accomplish this, normal brain endothelial cells were transduced by lentiviral vectors incorporating telomerase or SV40 T antigen. Among many stable immortalized clones obtained sequential limiting dilution cloning cells, one was selected for expression markers, including CD31, VE cadherin, and von Willebrand factor. This cell line, termed hCMEC/D3, showed karyotype, maintained contact-inhibited...
HepaRG cells, derived from a female hepatocarcinoma patient, are capable of differentiating into biliary epithelial cells and hepatocytes. More importantly, differentiated able to maintain activities many xenobiotic-metabolizing enzymes, expression the metabolizing enzyme genes can be induced by xenobiotics. The ability these express induce enzymes is in stark contrast frequently used HepG2 cells. previous studies have mainly focused on set selected genes; therefore, it significant interest...
Leukocyte migration into the central nervous system (CNS) is mediated by chemokines expressed on CNS endothelial cell surfaces. This study investigated production of and expression chemokine receptors human brain cells (HBECs) in vitro situ. Four (CCL2, CCL5, CXCL8, CXCL10) were demonstrated immunohistochemistry samples from patients with multiple sclerosis. CXCL8 CCL2 constitutively released increased primary HBECs line hCEMC/D3 response to tumor necrosis factor and/or interferon gamma....
The aim of this work was to conduct a comprehensive study about the transport properties NSAIDs across blood-brain barrier (BBB) in vitro. Transport studies with celecoxib, diclofenac, ibuprofen, meloxicam, piroxicam and tenoxicam were accomplished Transwell models based on cell line PBMEC/C1-2, ECV304 or primary rat brain endothelial cells. Single as well group substance carried out. In compositions, medium serum content varied, inhibitors verapamil probenecid added. Resulted permeability...
The blood-brain barrier (BBB) maintains the homeostasis between central nervous system and blood circulation [1]. Until now, no standard in vitro model of BBB is available. In this contribution a novel based on primary rat brain microvasculature endothelial cells (RBMEC) glial (RGC) introduced. were isolated from Wistar rats provided by company Biopredic Int. RBMEC co-cultured with RGC 12-well Transwell system. development was monitored measurement transendothelial electrical resistance (TEER).[...]