A5067345812- Barrier Structure and Function Studies
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Gut microbiota and health
- Liver Disease Diagnosis and Treatment
- Clostridium difficile and Clostridium perfringens research
- Connexins and lens biology
- Reproductive System and Pregnancy
- Diagnosis and Treatment of Venous Diseases
- Neonatal and fetal brain pathology
- Cell Adhesion Molecules Research
- Mosquito-borne diseases and control
- Microbial Metabolism and Applications
- Venous Thromboembolism Diagnosis and Management
- S100 Proteins and Annexins
- Immune Response and Inflammation
- Advanced Neuroimaging Techniques and Applications
- Blood transfusion and management
- COVID-19 epidemiological studies
- Hepatitis Viruses Studies and Epidemiology
- Immunotherapy and Immune Responses
- Blood groups and transfusion
- Atomic and Subatomic Physics Research
- Cancer Research and Treatments
- Yersinia bacterium, plague, ectoparasites research
Charité - Universitätsmedizin Berlin
2016-2019
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
2014
Institut für Medizinische Biometrie, Informatik und Epidemiologie
2010
University of Tübingen
1997
Clostridium perfringens enterotoxin (CPE) causes food poisoning and antibiotic-associated diarrhea. It uses some claudin tight junction proteins (eg, claudin-4) as receptors to form Ca2+-permeable pores in the membrane, damaging epithelial cells small intestine colon. We demonstrate that only a subpopulation of colonic enterocytes which are characterized by apical dislocation claudins CPE-susceptible. CPE-mediated damage was enhanced if paracellular barrier impaired Ca2+ depletion,...
Abstract Zinc homoeostasis exerts protective effects in inflammatory intestinal diseases and zinc supplementation has been successfully used for treating infectious diarrhoea. This study aimed at a characterisation of on focal leak induction by α-haemolysin (HlyA)-producing Escherichia coli (E. coli) as mechanism colitis. We conducted vivo experiments oral challenge gnotobiotic mice colonised with HlyA-expressing E. -536. Mice were either fed defined normal or high diet to analyse...
Clostridium perfringens enterotoxin (CPE) can be used to eliminate carcinoma cells that overexpress on their cell surface CPE receptors – a subset of claudins (e.g., Cldn3 and Cldn4). However, cannot target tumors expressing solely CPE‐insensitive (such as Cldn1 Cldn5). To overcome this limitation, structure‐guided modifications were generate variants strongly bind Cldn1, Cldn2 and/or Cldn5, while maintaining the ability Cldn4. This enabled (a) targeting most frequent endocrine malignancy,...
Early diagnosis of chronic hepatitis B virus (HBV) and C (HCV) infections is pivotal for optimal disease management. Sensitivity specificity 19 rapid diagnostic test (RDT) kits by different manufacturers (ABON, CTK Biotech, Cypress Diagnostics, Green Gross, Human Diagnostic, Humasis, InTec, OraSure, SD Bioline, Wondfo) were assessed on serum samples 270 Mongolians (90 seropositive surface antigen (HBsAg), 90 antibody (HCV-Ab), healthy subjects). All tested RDTs detection HBsAg performed with...
The majority of malignant tumors originate from epithelial cells, and many them are characterized by an overexpression claudins (Cldns) their mislocalization out tight junctions. We utilized the C-terminal claudin-binding domain Clostridium perfringens enterotoxin (cCPE), with its high affinity to specific members claudin family, as targeting unit for a claudin-sensitive cancer biosensor. To overcome poor sensitivity conventional relaxivity-based magnetic resonance imaging (MRI) contrast...
Claudins regulate paracellular permeability in different tissues. The claudin-binding domain of Clostridium perfringens enterotoxin (cCPE) is a known modulator claudin subset. However, it does not efficiently bind to claudin-1 (Cldn1). Cldn1 pharmacological target since (i) an essential co-receptor for hepatitis C virus (HCV) infections and (ii) key element the epidermal barrier limiting drug delivery. In this study, we investigated potential Cldn1-binding cCPE mutant inhibit HCV entry into...
Zusammenfassung Ziel: Zur Kompressionstherapie, die bei der chronischen venösen Insuffizienz als phlebologisches Basistherapeutikum gilt, werden Serienprodukte für unterschiedliche Unterschenkelumfänge angeboten. Direkt am Patienten sollte geprüft werden, ob unterschiedlichen Konfektionsgrößen entsprechend den verschiedenen Unterschenkelumfängen einen konstanten Anpressdruck ausüben. Material und Methoden: 50 Gefäßgesunde wurden in Studie aufgenommen (25 Frauen, 25 Männer, Alter 26,7 Jahre,...
Hintergrund: Das Denguefieber ist in tropischen Gebieten eine der häufigsten vektorübertragenen Virusinfektionen. In den betroffenen leben rund 3,61 Milliarden Menschen. Jährlich erkranken 36 Millionen an dem Virus, davon leiden 2,1 Menschen einer schweren Verlaufsform des Denguefiebers. Es wird vermutet, dass die Verlaufsformen (Severe Dengue bzw. DHF/DSS) durch aufeinander folgende Infektionen mit heterologen Serotypen verursacht werden. dieser Arbeit soll ein Modell zur Verfügung gestellt...
Dieterich, H.-J.; Deschner, N.; Krüger, W.; Nohé, B.; Eichner, M.; Unertl, K. Author Information