A5050505504- Circadian rhythm and melatonin
- Hemodynamic Monitoring and Therapy
- Respiratory Support and Mechanisms
- Intensive Care Unit Cognitive Disorders
- Epigenetics and DNA Methylation
- Healthcare Technology and Patient Monitoring
- Airway Management and Intubation Techniques
- Cardiac, Anesthesia and Surgical Outcomes
- Non-Invasive Vital Sign Monitoring
- Asthma and respiratory diseases
- Sepsis Diagnosis and Treatment
- Family and Patient Care in Intensive Care Units
- Immune cells in cancer
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Innovations in Medical Education
- Immune Cell Function and Interaction
- Estrogen and related hormone effects
- Educational Games and Gamification
- Cardiac Arrest and Resuscitation
- Renal function and acid-base balance
- Behavioral Health and Interventions
- Food Allergy and Anaphylaxis Research
- Cardiac tumors and thrombi
- Genomics, phytochemicals, and oxidative stress
- Artificial Intelligence in Healthcare and Education
University of Manchester
2014-2025
Manchester University NHS Foundation Trust
2021-2025
Manchester Royal Infirmary
2014-2024
Manchester Academic Health Science Centre
2018-2023
National Health Service
2019
Health Education North West
2015
Salford Royal NHS Foundation Trust
2014
Royal Oldham Hospital
2014
LMC Diabetes & Endocrinology (Canada)
2014
The circadian clock regulates many aspects of immunity. Bacterial infections are affected by time day, but the mechanisms involved remain undefined. Here we show that loss core protein BMAL1 in macrophages confers protection against pneumococcal pneumonia. Infected mice both reduced weight and lower bacterial burden circulating blood. In vivo studies macrophage phagocytosis reveal increased ingestion following Bmal1 deletion, which was also seen vitro. BMAL1-/- exhibited marked differences...
The glucocorticoid receptor (GR) is a major drug target in inflammatory disease. However, chronic (GC) treatment leads to disordered energy metabolism, including increased weight gain, adiposity, and hepatosteatosis - all programs modulated by the circadian clock. We demonstrated that while antiinflammatory GC actions were maintained irrespective of dosing time, liver was significantly more sensitive during day. Temporal segregation action underpinned physical interaction GR with...
Pulmonary inflammatory responses lie under circadian control; however, the importance of mechanisms in underlying fibrotic phenotype is not understood. Here, we identify a striking change to these resulting gain amplitude and lack synchrony within pulmonary tissue. These changes result from an infiltration mesenchymal cells, important cell type pathogenesis fibrosis. Mutation core clock protein REVERBα cells exacerbated development bleomycin-induced fibrosis, whereas mutation club or myeloid...
Abstract Background Use of wearable vital signs sensors (WVSSs) to monitor hospitalised patients is growing but uncertainty exists about how such should be adopted into existing practice. The aim this observational study was determine the completeness data capture and accuracy measurements recorded by a suite WVSSs. implications using derive early warning scores also assessed. Methods Adult inpatients with Covid-19 wore four WVSSs recording heart rate/respiratory rate (HR/RR), oxygen...
Summary We describe the development and laboratory assessment of a refined prototype tactile feedback device for safe accurate application cricoid pressure. recruited 20 operating department practitioners compared their performance pressure on training simulator using both manual unaided technique. The significantly reduced spread applied force: average (SE) root mean squared error decreased from 8.23 (0.48) N to 5.23 (0.32) (p < 0.001). upwards bias in force also decreased, 2.30 (0.74)...
Robust inflammatory responses are critical to survival following respiratory infection, with current attention focused on the clinical consequences of Coronavirus pandemic. Epigenetic factors increasingly recognized as important determinants immune responses, and EZH2 is a prominent target due availability highly specific efficacious antagonists. However, very little known about role in myeloid lineage. Here, we show acts macrophages limit activation, neutrophils for chemotaxis. Selective...
BackgroundAssessing circadian rhythmicity from infrequently sampled data is challenging; however, these types of are often encountered when measuring transcripts in hospitalized patients.MethodsWe present ClinCirc. This method combines 2 existing mathematical methods (Lomb-Scargle periodogram and cosinor) sequentially designed to measure oscillations clinical data. The accuracy this was compared against 9 other using simulated frequently biological ClinCirc then evaluated 13 intensive care...
Glucocorticoids (GCs) act through the glucocorticoid receptor (GR, also known as NR3C1) to regulate immunity, energy metabolism and tissue repair. Upon ligand binding, activated GR mediates cellular effects by regulating gene expression, but some can occur rapidly without new transcription. Here, we show that GCs inhibit cell migration, in response both agonist antagonist binding. The inhibitory effect on migration is prevented knockdown with siRNA, confirming specificity, not actinomycin D...
Abstract The circadian clock controls the physiological function of tissues through regulation thousands genes in a cell‐type‐specific manner. core cellular is transcription–translation negative feedback loop, which can recruit epigenetic regulators to facilitate temporal control gene expression. Histone methyltransferase, mixed lineage leukemia 3 (MLL3) was reported be required for maintenance oscillations cultured cells. Here, we test role MLL3 organization whole animals. Using mice...
The problem of comparing the deviation from a target two or more treatments procedures arises now and again in medicine. Practitioners usually carry out t-test on loss function such as absolute error. We have adapted developed statistical methods to give normative methodology for deviation-from-target problems exemplify them by evaluating performance tactile feedback device. Parametric nonparametric analyses are compared contrasted. recommend inference about functions error, with permutation...
OBJECTIVES (BACKGROUND): To externally validate clinical prediction models that aim to predict progression invasive ventilation or death on the ICU in patients admitted with confirmed COVID-19 pneumonitis. DESIGN: Single-center retrospective external validation study. DATA SOURCES: Routinely collected healthcare data electronic patient record. Curated recorded for each admission purposes of U.K. Intensive Care National Audit and Research Centre (ICNARC). SETTING: The at Manchester Royal...
<title>Abstract</title> Background Use of wearable vital signs sensors to monitor hospitalised patients is growing but uncertainty exists about completeness data capture and accuracy measurements. Implications for track trigger systems are unclear. <bold>Methods</bold> In this observational study, adult inpatients with Covid-19 wore four recording heart rate/respiratory rate (HR/RR), oxygen saturation (SpO<sub>2</sub>), axillary temperature blood pressure (BP). Wearable vitals were paired...
Fluid therapy is universally administered in the management of patients with sepsis, however excessive cumulative fluid balance has been shown to result worse outcomes. Hyperoncotic albumin results both lower volumes and early balance, may reduce short-term mortality septic shock.
Since Stewart and Treves (1948) first reported lymphangiosarcoma in chronically oedematous arms following mastectomy, the condition has been extensively recorded. The is a further report with lymphangiograms. In 1951 patient, then aged 61, underwent right radical mastectomy for an undifferentiated carcinoma arising upper outer quadrant. Post-operative irradiation 240 kV was applied to axilla, supra- infraclavicular regions lower neck, dose of 3,770 R given over 28 days.