A5070693957- Radiation Therapy and Dosimetry
- Optical Imaging and Spectroscopy Techniques
- Non-Invasive Vital Sign Monitoring
- Prostate Cancer Treatment and Research
- Prostate Cancer Diagnosis and Treatment
- Advanced Radiotherapy Techniques
- Cancer, Hypoxia, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- HER2/EGFR in Cancer Research
- Cancer Research and Treatments
- Cell Image Analysis Techniques
- Medical Imaging Techniques and Applications
- Effects of Radiation Exposure
- Hemodynamic Monitoring and Therapy
- Glioma Diagnosis and Treatment
- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
- Liver physiology and pathology
- Gastric Cancer Management and Outcomes
- Cancer Cells and Metastasis
- Urologic and reproductive health conditions
- Advanced MRI Techniques and Applications
- Radiation Effects in Electronics
- Radiopharmaceutical Chemistry and Applications
- Bladder and Urothelial Cancer Treatments
University of Oxford
2013-2024
CRUK/MRC Oxford Institute for Radiation Oncology
2011-2023
Medical Research Council
2015-2021
Cancer Research UK
2015-2021
King's College London
2012
Guy's and St Thomas' NHS Foundation Trust
2012
Churchill Hospital
2011
University College London
2001-2005
We have developed a novel near-infrared optical topography system that can acquire images of functional activation in the human brain at 10 frames per second using 32 detectors. The image acquisition rate is inversely proportional to number detectors, so maximum four detectors 80Hz. 16 laser diode sources (8 785 and 8 850nm) are illuminated simultaneously, each avalanche photodiode records light from several same time. contribution source demultiplexed software fast Fourier transforms. This...
Article15 October 2019Open Access Source DataTransparent process The p97–Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8 Abhay Narayan Singh Department Oncology, Cancer Research UK/Medical Council Oxford Institute for Radiation University Oxford, UK Search more papers by this author Judith Oehler Ignacio Torrecilla Susan Kilgas Shudong Li Bruno Vaz Claire Guérillon Novo Nordisk Foundation Center Protein Research, Copenhagen, Denmark John Fielden...
Abstract Purpose Prostate-specific membrane antigen (PSMA) is increasingly used to image prostate cancer in clinical practice. We sought develop and test a humanised PSMA minibody IAB2M conjugated the fluorophore IRDye 800CW-NHS ester men undergoing robot-assisted laparoscopic radical prostatectomy (RARP) cells during surgery. Methods The was evaluated pre-clinically using positive/negative xenograft models, following which 23 RARP between 2018 2020 received 2.5 mg 20 of IR800-IAB2M...
Yeung, Trevor M. MBBChir, MA, MRCS, DPhil; Volpi, Davide PhD; Tullis, Iain D. C. Nicholson, Gary A. MD; Buchs, Nicolas Cunningham, Chris MD, RCSEd; Guy, Richard FRCS; Lindsey, Ian MBBS, FRACS; George, Bruce MS, Jones, Oliver DM, Mun Wang, Lai MB, BCh, MRCP, FRCPath; Hompes, Roel Vojnovic, Borivoj Hamdy, Freddie FRCSEd (Urol), FRCS, FMedSci; Mortensen, Neil J. FRCS (Eng), Hon FRCPS (Glas), (Ed) Author Information
Sentinel Lymph Node (SLN) sampling may significantly reduce surgical morbidity by avoiding needless radical lymphadenectomy. In gynaecological cancers, the current practice in UK is testing accuracy of SLN detection using radioactive isotopes within context clinical trials. However, tracers pose significant logistic problems. We, therefore, conducted a pilot, observational study to assess feasibility novel optical imaging device for cancers near infrared (NIR) fluorescence. A novel,...
Summary We describe a microscopy design methodology and details of microscopes built to this ‘open’ approach. These demonstrate the first implementation time‐domain fluorescence in flexible automated platform with ability ease transition other advanced techniques from development use routine biology applications. This approach allows easy expansion modification capabilities, as it moves away commercial, monolithic, microscope body small, commercial off‐the‐shelf custom made modular...
Iron is vital to living cells, playing a key role in cellular respiration, DNA synthesis, and various metabolic functions. Importantly, cancer cells have higher dependency on iron compared normal support their rapid growth survival. Due this fact, tumors are more vulnerable ferroptosis, an iron-dependent form of regulated cell death. Radiation therapy (RT), standard treatment for many patients, known induce ferroptosis. Ultra-high dose rate FLASH RT offers improved therapeutic window by...
Abstract Background FLASH radiotherapy using megavoltage (MV) photon beams should enable greater therapeutic efficacy, target deep seated tumors, and provide insights into mechanisms within FLASH. Purpose In this study, we aim to show how facilitate ultra‐high dose rates (FLASH) with MV photons over a field size of 12–15 mm, 6 MeV (nominal) preclinical electron linear accelerator (linac). Our intention is utilize setup deliver in future experiments. Methods An operating at pulse repetition...
Extension of prostate cancer beyond the primary site by local invasion or nodal metastasis is associated with poor prognosis. Despite significant research on tumour evolution in metastasis, emergence and clones at this early stage expansion spread are poorly understood. We aimed to delineate routes within seminal vesicles lymph nodes, linking these histological features that used diagnostic risk stratification.
Functional imaging can provide a level of quantification that is not possible in what might be termed traditional high-content screening. This due to the fact current state-of-the-art screening systems take approach scaling-up single cell assays, and are therefore based on essentially pictorial measures as assay indicators. Such phenotypic analyses have become extremely sophisticated, advancing enormously, but this still somewhat subjective. We describe development, validation, prototype...
The addition of fluorescence guidance in laparoscopic procedures has gained significant interest recent years, particularly through the use near infrared (NIR) markers.In this work we present a novel laparoscope camera coupler based on an electrically tunable fluidic lens that permits programmable focus control and desirable achromatic performance from visible to NIR.Its extends lower working distance limit improves detection sensitivity, important for with molecularly targeted markers.We...
The Surrey vertical beam is a new facility for targeted irradiation of cells in medium with singly counted ions. A duo-plasmatron ion source and 2 MV Tandem™ accelerator supply range ions from protons to calcium this beamline microscope endstation, energy ranges 0.5 12 MeV. magnetic quadrupole triplet lens used focus the We present design beamline, early results showing capability count single 98% certainty on CR-39 track etch. also show that targeting accuracy within 5 μm selectively target...
Benjamin R. Waterhouse 1* , Merel Gijsen Paul Barber 2 Iain D.C. Tullis Borivoj Vojnovic and Anthony Kong 1 Human Epidermal Growth Factor Receptor Group; Cancer Research UK Molecular Oncology Laboratories, The Weatherall Institute of Medicine (WIMM), University Oxford, John Radcliffe Hospital, OX3 9DS, UK. Gray for Radiation Biology, Old Road Campus Building, Off Roosevelt Drive, Churchill 7DQ, * Indicates equal contribution Received: August 26, 2011; Accepted: September 6, Published: 8,...
The development of image-guided small animal irradiators represents a significant improvement over standard by enabling preclinical studies to mimic radiotherapy in humans. ability deliver tightly collimated targeted beams, conjunction with gantry or couch rotation, has the potential maximize tumor dose while sparing normal tissues. However, current commercial platforms do not incorporate respiratory gating, which is required for accurate and precise targeting organs subject respiration...
Objective: Neuroblastoma has one of the lowest survival rates all childhood cancers, despite use intensive treatment regimens. Preclinical models neuroblastoma are essential for testing new multimodality protocols, including those that involve radiotherapy (RT). The aim this study was to develop a robust method RT planning and tumour response monitoring based on combined MRI cone-beam CT (CBCT) imaging apply it widely studied mouse xenograft model neuroblastoma, SK-N-SH. Methods: As part...
A portable topographic near-infrared spectroscopic (NIRS) imaging system has been developed to provide real-time temporal and spatial information about the cortical response stimulation in unrestrained infants. The optical sensing array is lightweight, flexible, easy apply infants ranging from premature babies intensive care children a normal environment. sensor pad consists of flexible double-sided circuit board onto which are mounted multiple sources (light-emitting diodes) detectors...
The intracellular distribution of Auger electron–emitting radiopharmaceuticals is a determinant cytotoxicity. However, the mechanisms by which these agents are routed through cell ill understood. aim this study was to investigate how trafficking <sup>111</sup>In-labeled human epidermal growth factor (<sup>111</sup>In-DTPA-hEGF) relates that EGF receptor (EGFR) and whether coadministration modulate EGFR signaling alters efficacy <sup>111</sup>In-DTPA-hEGF. <b>Methods:</b> spatiotemporal...
Abstract When relativistic electrons are used to irradiate tissues, such as during FLASH pre-clinical irradiations, the electron beam energy is one of critical parameters that determine dose distribution. Moreover, linear accelerators (linacs) usually operate with significant loading, where a small change in accelerator output current can lead reduction. Optimisation tuning accelerator’s radio frequency system often required. We describe here robust, easy-to-use device for non-interceptive...
There is a need to improve the treatment of prostate cancer (PCa) and reduce side effects. Vascular-targeted photodynamic therapy (VTP) focal for low-risk low-volume localised PCa, which rapidly disrupts targeted tumour vessels. interest in expanding use VTP higher-risk disease. Tumour vasculature characterised by vessel immaturity, increased permeability, aberrant branching inefficient flow. FRT alters microenvironment promotes transient 'vascular normalisation'. We hypothesised that...
BACKGROUND Standard biomarker testing of a single macroscopic disease site is unlikely to be sufficient because tumor heterogeneity. A focus on examining global expression or activity, particularly in microscopic residual chemotherapy-resistant disease, needed for the appropriate selection targeted therapies. This study was aimed at establishing technique assessment biomarkers ovarian cancer peritoneal spread. METHODS An in-house developed fluorescent imaging device used detect c-Met...
Introduction Preclinical CT-guided radiotherapy platforms are increasingly used but the CT images characterized by poor soft tissue contrast. The aim of this study was to develop a robust and accurate method MRI-guided (MR-IGRT) delivery abdominal targets in mouse. Methods A multimodality cradle developed for providing subject immobilisation its performance evaluated. Whilst still dose calculations, target identification based on MRI. Each step planning procedure validated initially vitro...