A5017932546- Advanced MRI Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Ultrasound and Hyperthermia Applications
- MRI in cancer diagnosis
- Radiopharmaceutical Chemistry and Applications
- Pancreatic and Hepatic Oncology Research
- Medical Imaging Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Cancer Research and Treatments
- Advanced NMR Techniques and Applications
- Lanthanide and Transition Metal Complexes
- Peptidase Inhibition and Analysis
- Electron Spin Resonance Studies
- Immune cells in cancer
- Hepatocellular Carcinoma Treatment and Prognosis
- Advanced Radiotherapy Techniques
- Nanoplatforms for cancer theranostics
- Caveolin-1 and cellular processes
- Advanced Neuroimaging Techniques and Applications
- Atomic and Subatomic Physics Research
- Metabolomics and Mass Spectrometry Studies
- Cardiac Imaging and Diagnostics
- Angiogenesis and VEGF in Cancer
- Radiation Therapy and Dosimetry
- Photoacoustic and Ultrasonic Imaging
University of Oxford
2015-2025
Wellcome Centre for Integrative Neuroimaging
2023-2025
CRUK/MRC Oxford Institute for Radiation Oncology
2013-2023
Cancer Research UK
2012-2022
Medical Research Council
2011-2020
Churchill Hospital
2016
University of Surrey
2013
Science Oxford
2010-2013
University of Cambridge
2013
Imperial College London
2013
BACKGROUND Dysfunction after thrombolytic therapy of acute myocardial infarction (MI) may be reversible. Early infarction, both reversible and irreversible injury manifested by regional wall motion abnormalities. Improved thickening during dobutamine infusion (dobutamine-responsive motion) accurately identify reversibly injured segments. METHODS AND RESULTS To determine whether dobutamine-responsive detects postischemic dysfunction irrespective infarct location, multistage (baseline, 4 12...
Because metastasis is associated with the majority of cancer-related deaths, its prevention a clinical aspiration. Prostanoids are large family bioactive lipids derived from activity cyclooxygenase-1 (COX-1) and COX-2. Aspirin impairs biosynthesis all prostanoids through irreversible inhibition both COX isoforms. Long-term administration aspirin leads to reduced distant metastases in murine models trials, but isoform, downstream prostanoid, cell compartment responsible for this effect yet be...
Liver metastasis from colorectal cancer is a leading cause of mortality. Myeloid cells play pivotal roles in the metastatic process, but their prometastatic functions liver remain incompletely understood. To investigate role, we simulated C57BL/6 mice through intrasplenic inoculation MC38 colon carcinoma cells. Among heterogeneous myeloid infiltrate, identified distinct population CD11b/Gr1mid different other populations previously associated with metastasis. These increased number...
Hepatic metastases are amenable to ablation; however, many patients not suitable candidates for such therapy and recurrence is common. The tumor microenvironment known be essential metastatic growth, yet identification of plausible targets cancer in the has proven elusive. We found that human colorectal liver murine gastrointestinal experimental infiltrated by neutrophils. Plasticity neutrophils recently been shown lead both protumor antitumor effects. Here, promoted growth hepatic...
Abstract Translocator protein (TSPO) expression is increased in activated glia, and has been used as a marker of neuroinflammation PET imaging. However, the extent to which TSPO upregulation reflects pro‐ or anti‐inflammatory phenotype remains unclear. Our aim was determine whether astrocytes microglia/macrophages limited specific inflammatory phenotype. assessed by flow cytometry cultured astrocytes, microglia, macrophages stimulated with lipopolysaccharide (LPS), tumor necrosis factor...
Poly(ADP-ribose) polymerase (PARP) inhibitors are increasingly being studied as cancer drugs, single agents, or a part of combination therapies. Imaging PARP using radiolabeled inhibitor has been proposed for patient selection, outcome prediction, dose optimization, genotoxic therapy evaluation, and target engagement imaging novel PARP-targeting agents. <b>Methods:</b> Here, via the copper-mediated <sup>18</sup>F-radiofluorination aryl boronic esters, we accessed, first time (to our...
The 18F-labeling of 5-(trifluoromethyl)-dibenzothiophenium trifluoromethanesulfonate, commonly referred to as the Umemoto reagent, has been accomplished applying a halogen exchange 18F-fluorination with 18F-fluoride, followed by oxidative cyclization Oxone and trifluoromethanesulfonic anhydride. This new 18F-reagent allows for direct chemoselective unmodified peptides at thiol cysteine residue.
<sup>64</sup>Cu-diacetyl-bis(<i>N</i><sup>4</sup>-methylthiosemicarbazonate), <sup>64</sup>Cu-ATSM, continues to be investigated clinically as a PET agent both for delineation of tumor hypoxia and an effective indicator patient prognosis, but there are still aspects the mechanism action that not fully understood. <b>Methods:</b> The retention radioactivity in tumors after administration <sup>64</sup>Cu-ATSM vivo is substantially higher with significant hypoxic fraction. This...
Abnormal pH is a common feature of malignant tumors and has been associated clinically with suboptimal outcomes. Amide proton transfer magnetic resonance imaging (APT MRI) holds promise as means to noninvasively measure tumor pH, yet multiple factors collectively make quantification from APT MRI data challenging. The purpose this study was improve our understanding the biophysical sources altered signals in tumors. Combining
Significance Acidic pH may distinguish aggressive from more indolent cancers. The limitation on testing this hypothesis to date has been the difficulty of measuring acidic in Here we show that retention low insertion peptide (pHLIP) Variant 3 (Var3) reflects pH. Using pHLIP Var3, its ability detect cancer with a false-positive rate genetically engineered model murine breast cancer, paving way for probe clinical situations.
Abstract Purpose: To describe a combination of techniques using the excellent volumetric capacities magnetic resonance imaging (MRI) while avoiding anesthesia and maintaining high‐throughput capability for tumor volume measurement in awake mouse. This approach presents an alternative to calipers which, although cheap, fast, easy use, introduce many biases estimation. Materials Methods: The murine CaNT subcutaneous xenograft model was used. A quiet modestly T2‐weighted spin‐echo scan acquired...
Abstract Gemcitabine constitutes one of the backbones for chemotherapy treatment in pancreatic ductal adenocarcinoma (PDAC), but patients often respond poorly to this agent. Molecular markers downstream gemcitabine preclinical models may provide an insight into resistance mechanisms. Using cytokine arrays, we identified potential secretory biomarkers (response) transgenic KRasG12D; Trp53R172H; Pdx-1 Cre (KPC) mouse model PDAC. We verified oncogenic role tissue inhibitor matrix...
Angiogenesis is an essential component of tumour growth and, consequently, important target both therapeutically and diagnostically. The cell adhesion molecule α(v)β(3) integrin a specific marker angiogenic vessels the most prevalent vascular that binds amino acid sequence arginine-glycine-aspartic (RGD). Previous studies using RGD-targeted nanoparticles (20-50 nm diameter) iron oxide (NPIO) for magnetic resonance imaging (MRI) angiogenesis, have identified number limitations, including...
Purpose Hyperpolarized metabolic imaging has the potential to revolutionize diagnosis and management of diseases where metabolism is dysregulated, such as heart disease. We investigated feasibility rodent myocardial at high resolution 7 T. Methods present here a fly‐back spectral‐spatial radiofrequency pulse that sidestepped maximum gradient strength requirements enabled myocardium. A 3D echo‐planar readout followed, with centric ordered z ‐phase encoding. The cardiac gated sequence was used...
CT is widely used for anatomic referencing of PET and SPECT images small animals but requires sufficiently high radiation doses capable causing significant DNA damage. Therefore, we described the relationship between dose, biologic damage, image quality to determine whether can be without significantly compromising radiotherapy tumor development studies. <b>Methods:</b> The dose index generated by nanoSPECT/CT system was compared with measurements using EBT2 gafchromic film. effects micro-CT...
Myeloid cells are known to mediate metastatic progression. Here, we attempted elucidate the mechanisms underlying these effects by identifying gene expression alterations in cancer forming hepatic metastases after myeloid cell depletion. Hepatic heavily infiltrated CD11b + cells. We established transgenic CD11b‐diphtheria toxin receptor mice intrasplenic injection of MC38 colon and Lewis lung carcinoma before depleting with diphtheria toxin. depletion inhibited growth a marked diminishment...
Nanomedicines allow active targeting of cancer for diagnostic and therapeutic applications through incorporation multiple functional components. Frequently, however, clinical translation is hindered by poor intratumoural delivery distribution. The application physical stimuli to promote tumour uptake a viable route overcome this limitation. In study, ultrasound-mediated cavitation microbubbles was investigated as mean enhancing the liposome designed chemo-radionuclide therapy targeted EGFR...
Molecular radiotherapy using <sup>177</sup>Lu-DOTATATE is a most effective treatment for somatostatin receptor–expressing neuroendocrine tumors. Despite its frequent and successful use in the clinic, little or no radiobiologic considerations are made at time of planning delivery. On positive uptake on octreotide-based PET/SPECT imaging, usually administered as standard dose number cycles without adjustment peptide uptake, dosimetry, DNA damage effects tumor. Here, we visualized quantified...
The purpose of this study was to evaluate the temporal and spatial pathological alterations within ischemic tissue using serial magnetic resonance imaging (MRI) determine extent duration functional impairment objective behavioral tests after transient middle cerebral artery occlusion (tMCAO) in rat. MRI signatures derived from specific anatomical regions interest (ROI) were then appropriately correlated measures over time course (up 28 days post-tMCAO). Sprague-Dawley rats (n = 12) initially...
Prostate adenocarcinoma (CaP) patients are classified into low-, intermediate-, and high-risk groups that reflect relative survival categories. While there accepted treatment regimens for low- patients, intermediate-risk pose a clinical dilemma, as outcomes highly variable these individuals. A better understanding of the factors regulate progression CaP is required to delineate risk. For example, aberrant activation Hedgehog (Hh) pathway implicated in progression. Here, we identify serine...