A5087890448- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Signaling Pathways in Disease
- Bacterial Genetics and Biotechnology
- Cellular Mechanics and Interactions
- CRISPR and Genetic Engineering
- Force Microscopy Techniques and Applications
- HER2/EGFR in Cancer Research
- RNA and protein synthesis mechanisms
- Molecular Junctions and Nanostructures
- DNA Repair Mechanisms
- Wildlife Ecology and Conservation
- Bacteriophages and microbial interactions
- Blood Coagulation and Thrombosis Mechanisms
- Angiogenesis and VEGF in Cancer
- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- Genetic factors in colorectal cancer
- Melanoma and MAPK Pathways
- Collagen: Extraction and Characterization
- Nanofabrication and Lithography Techniques
- Mollusks and Parasites Studies
- Coccidia and coccidiosis research
University of Malakand
2019-2025
Wayne State University
2010-2024
University of Gujrat
2023
The Barbara Ann Karmanos Cancer Institute
2007-2021
UConn Health
2002
New England Biolabs (United States)
1995
University of the Punjab
1987-1993
Centre of Excellence in Molecular Biology
1993
Cold Spring Harbor Laboratory
1986-1990
Activation-induced cytidine deaminase (AID) is required for the maturation of antibodies in higher vertebrates, where it promotes somatic hypermutation (SHM), class switch recombination and gene conversion. While known that SHM requires high levels transcription target genes, unclear whether this because AID targets transcribed genes. We show here human C to T mutations Escherichia coli which are stimulated by transcription. The strand-biased occur preferentially non-transcribed strand gene....
The membrane type (MT)-matrix metalloproteinases (MMPs) constitute a subgroup of membrane-anchored MMPs that are major mediators pericellular proteolysis and physiological activators pro-MMP-2. MT-MMPs also exhibit differential inhibition by members the tissue inhibitor metalloproteinase (TIMP) family. Here we investigated processing, catalytic activity, TIMP MT3-MMP (MMP-16). Inhibitor profile mutant enzyme studies indicated is regulated on cell surface autocatalytic processing ectodomain...
Deamination of 5-methylcytosine in DNA results T/G mismatches. If unrepaired, these mismatches can lead to C-to-T transition mutations. The very short patch (VSP) repair process Escherichia coli counteracts the mutagenic by repairing favor G-containing strand. Previously we have shown that a plasmid containing an 11-kilobase fragment from E. chromosome complement chromosomal mutation defective both cytosine methylation and VSP repair. We now mapped regions essential for two phenotypes. In...
BackgroundDiscoidin domain receptor 1 (DDR1) is a tyrosine kinase that activated by collagens involved in the pathogenesis of fibrotic disorders. Interestingly, de novo production collagen type I (Col I) has been observed Col4a3 knockout mice, mouse model Alport Syndrome (AS mice). Deletion DDR1 AS mice was shown to improve survival and renal function. However, mechanisms driving DDR1-dependent fibrosis remain largely unknown.MethodsPodocyte pDDR1 levels, Collagen cluster differentiation 36...
MMP25 (MT6-MMP) is one of the two glycosylphosphatidylinositol-anchored matrix metalloproteinases (MMPs) that have been suggested to play a role in pericellular proteolysis. However, its cancer unknown, and biochemical properties are not well established. Here we found marked increase MT6-MMP expression within situ dysplasia invasive 61 samples human colon cancer. Expression HCT-116 cells promoted tumori-genesis nude mice. Histologically, MT6-MMP-expressing tumors demonstrated an...
MBD4 is a member of the methyl-CpG-binding protein family. It contains two DNA binding domains, an amino-proximal methyl-CpG domain (MBD) and C-terminal mismatch-specific glycosylase domain. Limited <i>in vitro</i> proteolysis mouse yields stable fragments: 139-residue fragment including MBD, other 155-residue Here we show that latter active as on duplex containing G:T mismatch within CpG sequence context. The crystal structure confirmed helix-hairpin-helix superfamily. site situated in...
Abstract The discoidin domain receptor 1 (DDR1) is a member of the tyrosine kinase family that signals in response to collagen and has been implicated cancer progression. In present study, we investigated expression role DDR1 human melanoma Immunohistochemical staining specimens ( n = 52) shows high lesions correlates with poor prognosis. was associated clinical characteristics Clark level ulceration BRAF mutations. Downregulation by small interfering RNA (siRNA) vitro inhibited cells...
Grewia optiva leaf extract was used as a reducing and stabilizing agent to create iron nanoparticles (FeNPs) in an eco-friendly manner. Before being extracted aqueous solution for 20 min at 100 ºC using Jeldal equipment, the leaves were meticulously dried, washed, dried again. The dissolve (III) chloride (FeCl₃) bio-fabricate (FeNPs). Temperature, duration, pH, salt effect parame-ters optimize bio-fabricated FeNPs. It found that temperature of 85°C, pH ranging from 6 7, 24-h duration ideal...
The dcm locus of Escherichia coli K-12 has been shown to code for a methylase that methylates the second cytosine within sequence 5'-CC(A/T)GG-3'. This is also recognized by EcoRII restriction-modification system coded E. plasmid N3. same as protein. We have isolated, from library DNA, two overlapping clones carry locus. show sequences are present in dcm+ strain, but absent delta strain. cloned gene codes methylase, it complements mutations and protects recognition sites cleavage...
Abstract The Discoidin Domain Receptors (DDRs) constitute a unique set of receptor tyrosine kinases that signal in response to collagen. Using an inducible expression system human HT1080 fibrosarcoma cells, we investigated the role DDR1b and DDR2 on primary tumour growth experimental lung metastases. Neither nor altered at site. However, implantation DDR1b- or DDR2-expressing cells with collagen I significantly accelerated rate, effect could not be observed absence DDR induction....
MT4-MMP (MMP17) belongs to a unique subset of membrane type-matrix metalloproteinases that are anchored the cell surface via glycosylphosphatidylinositol moiety. However, little is known about its biochemical properties. Here, we report displayed on as mixed population monomeric, dimeric, and oligomeric forms. Sucrose gradient fractionation demonstrated these forms all present in lipid rafts. Mutational computational analyses revealed Cys(564), which within stem region, mediates...
ADAM17 is implicated in several debilitating diseases. However, drug discovery efforts targeting have failed due to the utilization of zinc-binding inhibitors. We previously reported highly selective nonzinc-binding exosite-targeting inhibitors that exhibited not only enzyme isoform selectivity but synthetic substrate as well (J. Biol. Chem. 2013, 288, 22871). As a result SAR studies presented herein, we obtained inhibitors, six which were further characterized biochemical and cell-based...
The BamHI restriction-modification system contains a third gene, bamHIC, which positively regulates bamHIR. Similar small genes from other systems were tested in vivo for their ability to cross-complement. C.BamHI protein was identified, purified, and used raise polyclonal antibodies. Attempts detect C proteins cell extracts by cross-reactivity with antibodies proved unsuccessful.
The membrane type (MT) 6 matrix metalloproteinase (MMP) (MMP25) is a glycosylphosphatidylinositol-anchored that highly expressed in leukocytes and some cancer tissues. We previously showed natural MT6-MMP on the cell surface as major reduction-sensitive form of M(r) 120, likely representing enzyme homodimers held by disulfide bridges. Among type-MMPs, stem region contains three cysteine residues at positions 530, 532, 534 which may contribute to dimerization. A systematic site-directed...
Polymorphonuclear neutrophils (PMNs) are the first line of defense against invading organisms in humans; addition, PMNs contribute to linking innate and adaptive immunity. To fulfill their biological behavior, utilize an arsenal proteolytic enzymes, including members matrix metalloproteinase family zinc-dependent endopeptidases. express high levels MT6-MMP (MMP-25), a glycosyl-phosphatidylinositol-anchored MMP, that belongs subfamily membrane-anchored metalloproteinases. Due paucity...