The remarkable host specificity of the species genus Actinobaculum led us to recharacterize these by a polyphasic approach. A comparative chemotaxonomic study including analysis whole-cell sugars, amino acid composition peptidoglycan, fatty methyl esters, respiratory quinones and polar lipids revealed significant differences that, in combination with molecular data, support dissection Actinobaculum. proposals this include reclassification schaalii urinale as Actinotignum gen. nov., comb....

Chemotaxonomic and 16S rRNA gene sequence analyses of an isolate from the bronchial secretions a patient with chronic bronchitis demonstrated clearly that it belongs to genus Nocardia. The data, as well biochemical characteristics isolate, indicated new species differs previously described members name Nocardia cyriacigeorgici sp. nov. is proposed for this represented by strain IMMIB D-1627T (= DSM 44484T).

The taxonomic status of a light-orange-coloured bacterial isolate from an oil-contaminated soil sample was characterized by using polyphasic approach. Comparative analysis 16S rRNA gene sequences demonstrated that the belonged phylogenetically to genus Azospirillum, with Azospirillum canadense, brasilense and doebereinerae as its closest phylogenetic relatives (97.3, 97.0 % similarity, respectively). DNA–DNA pairing studies showed unidentified organism displayed 25.0, 17.0 19.0 relatedness...

Chemotaxonomic and genomic 16S ribosomal DNA sequence analyses of two isolates obtained from different clinical materials clearly delineated a new species the genus Tsukamurella. This can be identified by its similarity values, as well physiological characteristics. The name Tsukamurella inchonensis sp. nov. is proposed for these isolates, which are represented strain IMMIB D-771T (= DSM 44067T) (T = type strain). exhibits only 45% relatedness to paurometabola.

Chemotaxonomic and 16S rRNA gene sequence analyses of four bacterial strains isolated from clinical material clearly demonstrated that these bacteria belong to the genus Nocardia. DNA-DNA hybridization data as well physiological characteristics isolates indicated they are closely related a single species differs previously described members genus. The name Nocardia abscessus sp. nov. is proposed for organisms represented by strain IMMIB D-1592T (= DSM 44432T). Strain exhibits 56.8 60.0%...

A new species of the genus Nocardiopsis, for which we propose name Nocardiopsis lucentensis sp. nov. (type strain, strain DSM 44048), was isolated from a salt marsh soil sample near Alicante, Spain. Whole-cell hydrolysates contain meso isomer diaminopimelic acid and no characteristic sugar; thus, cell wall composition is type III. Menaquinone MK-10(H8) major menaquinone, phospholipid PHI (phosphatidylcholine present). Spore chains are rectiflexibilis, in early stages sporulation...

Chemotaxonomic and 16S ribosomal DNA sequence analyses of four bacterial isolates from blood cultures patients with cardiac pacemaker implants sputa chronic lung infections clearly demonstrated that these bacteria belong to the genus Tsukamurella. DNA-DNA hybridization data, as well physiological characteristics isolates, indicate they are closely related a single species differs previously described members The name Tsukamurella tyrosinosolvens sp. nov. is proposed for new represented by...

Chemotaxonomic and 16S rDNA sequence analyses of an isolate from the sputa bronchial secretions a patient with chronic lung disease clearly demonstrated that it belongs to genus Nocardia. DNA-DNA hybridization data, as well biochemical characteristics isolate, indicate new species differs previously described members The name Nocardia paucivorans sp. nov. is proposed for this represented by strain IMMIB D-1632T (= DSM 44386T).

Chemotaxonomic and 16S ribosomal DNA sequence analyses of an isolate from the sputum a patient with mycobacterial lung infection clearly delineated new species genus Tsukamurella. This can be defined on basis genotypic phenotypic data. The name Tsukamurella pulmonis sp. nov. is proposed for this organism; type strain IMMIB d-1321T (= DSM 44142T). shows 44.2 36.2% relatedness to paurometabola 20162T (T = strain) inchonensis 44067T, respectively.

The taxonomic status of a bacterium isolated from perianal swab patient with bone marrow transplant was characterized using polyphasic approach. Chemotaxonomic investigations revealed the presence cell wall chemotype IV, short chain mycolic acids that co-migrated those extracted members genus Dietzia, and dihydrogenated menaquinone eight isoprene units as predominant menaquinone. Generic assignment confirmed by 16S rRNA gene sequencing. Comparative analysis sequence showed this isolate...

A novel Gram-positive, aerobic, catalase-positive, filamentous micro-organism, designated strain IMMIB L-1269(T), originating from sputum was characterized using phenotypic and molecular taxonomic methods. It showed cell-wall chemotype III, phospholipid type PII (with phosphatidylethanolamine as the diagnostic phospholipid) contained an unsaturated menaquinone with seven isoprene units (MK-7) predominant isoprenoid quinone. synthesized long-chain cellular fatty acids of straight-chain...

A channel‐forming protein was identified in cell wall extracts of the Gram‐positive, strictly aerobic bacterium Nocardia farcinica . The porin purified to homogeneity and had an apparent molecular mass about 87 kDa on tricine‐containing SDS–PAGE. When boiled for a longer time sodium dodecylsulphate (SDS) it dissociated into two subunits with masses 19 23 kDa. form able increase specific conductance artificial lipid bilayer membranes from phosphatidylcholine (PC) phosphatidylserine (PS)...

A Gram-reaction-positive, aerobic, catalase-positive, non-spore-forming, rod-shaped bacterium designated IMMIB L-1656(T) was isolated from an ear swab of a man and characterized using polyphasic approach. 16S rRNA gene sequence analysis indicated that strain is related to members the family Micrococcaceae (<95.1 % similarity). Anaylsis different phylogenetic algorithms consistently grouped with genus Yaniella. The organism posessed cell-wall murein based on L-lysine (variation A4α, type...

A yellow-pigmented, Gram-reaction-negative bacterium isolated from a human clinical source was investigated using polyphasic approach in order to clarify its taxonomic status. Comparative 16S rRNA gene sequence analysis showed that the new isolate constituted distinct phyletic line within genus Chryseobacterium, displaying >2.8 % divergence with recognized species of this genus. The generic assignment confirmed by chemotaxonomic data which revealed fatty acid profile consisting...

An isolate that was received during a mycobacterial quality control test and which thought to be Mycobacterium species subjected polyphasic taxonomic study after mycolic acid analysis showed it possessed mycolates characteristic of the nocardiae. Further chemotaxonomic 16S rRNA gene sequence analyses this demonstrated belongs genus Nocardia. data, DNA-DNA hybridization studies biochemical characteristics indicate novel differs from previously described members The name Nocardia ignorata sp....

Severe postoperative wound infections caused by Nocardia farcinica were repeatedly observed in a German hospital surgical ward. A pulsed-field gel electrophoresis (PFGE) protocol was established to characterize the genetic relatedness of bacterial isolates from these infections. All 18 that have occurred since 1985 belong common endemic genotype; organisms this genotype also detected air two rooms department where occurred. In contrast, environmental another building on same campus showed...

Two coryneform bacteria isolated from human clinical specimens were characterized by phenotypic and molecular taxonomic methods. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV short-chain mycolic acids consistent with genus Corynebacterium sensu stricto. Comparative 16S rRNA gene sequence analysis showed that two strains are genealogically highly related (99.8% similarity) constitute a new subline within Corynebacterium, minutissimum as their nearest...

A bacterial isolate (IMMIB R-145(T)) from a human abscess was subjected to polyphasic taxonomic study. Chemotaxonomic investigations revealed the have cell wall chemotype IV and mycolic acids consistent with characteristics of genus NOCARDIA: Comparative 16S rRNA gene sequencing showed that constituted distinct subline within Nocardia, displaying greater than 1.7 % sequence divergence established species. However, DNA-DNA hybridization study demonstrated unambiguously genealogically closely...

A case of superficial wound infection in a noncompromised host due to Rhodococcus equi-like organism, acquired from soil, is described. The strain grew poorly at 37 degrees C and showed several differences wild-type R. equi biochemical tests its fatty acid mycolic compositions.

A lipophilic, coryneform bacterium isolated from a human clinical specimen was characterized by phenotypic and molecular-taxonomic methods. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV short-chain mycolic acids consistent with genus Corynebacterium. The isolate could be distinguished other members Corynebacterium positive urease catalase tests as well its failure to produce acid carbohydrates. Comparative 16S rRNA gene sequencing showed that this constitutes...

The taxonomic status of a Gram-negative-staining bacterium, isolated from the faeces seal, was investigated using polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that novel isolate formed distinct phyletic line within genus Psychrobacter, displaying >3.3% divergence with other known Psychrobacter species. generic assignment confirmed by chemotaxonomic data, which revealed fatty acid profile included straight-chain saturated, unsaturated and 3-hydroxylated acids,...