A5073722449- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Liver physiology and pathology
- Pluripotent Stem Cells Research
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Prostate Cancer Treatment and Research
- Liver Disease Diagnosis and Treatment
- Bone Tissue Engineering Materials
- Cancer Research and Treatments
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Prostate Cancer Diagnosis and Treatment
- Pediatric Hepatobiliary Diseases and Treatments
- Bone health and treatments
- Immune cells in cancer
- Drug Transport and Resistance Mechanisms
- Gallbladder and Bile Duct Disorders
- Urologic and reproductive health conditions
- Spectroscopy Techniques in Biomedical and Chemical Research
- Artificial Intelligence in Healthcare
- Spectroscopy and Chemometric Analyses
- Proteoglycans and glycosaminoglycans research
- CAR-T cell therapy research
- Osteoarthritis Treatment and Mechanisms
National University of Singapore
2016-2025
National University Cancer Institute, Singapore
2022-2025
Auckland City Hospital
2022
Southern Medical University
2020
Rice University
2012-2017
Yong In University
2016
Royal North Shore Hospital
2013
Tauranga Hospital
2006
Organoids have attracted increasing attention because they are simple tissue-engineered cell-based in vitro models that recapitulate many aspects of the complex structure and function corresponding vivo tissue. They can be dissected interrogated for fundamental mechanistic studies on development, regeneration, repair human tissues. also used diagnostics, disease modeling, drug discovery, personalized medicine. derived from either pluripotent or tissue-resident stem (embryonic adult)...
The pronounced biological influence of the tumor microenvironment on cancer progression and metastasis has gained increased recognition over past decade, yet most preclinical antineoplastic drug testing is still reliant conventional 2D cell culture systems. Although monolayer cultures recapitulate some phenotypic traits observed clinically, they are limited in their ability to model full range microenvironmental cues, such as ones elicited by 3D cell–cell cell–extracellular matrix...
Abstract Current liver fibrosis scoring by computer-assisted image analytics is not fully automated as it requires manual preprocessing (segmentation and feature extraction) typically based on domain knowledge in pathology. Deep learning-based algorithms can potentially classify these images without the need for through learning from a large dataset of images. We investigated performance classification models built using deep algorithm pre-trained multiple sources to score compared them...
The lack of effective therapies for bone metastatic prostate cancer (PCa) underscores the need accurate models disease to enable discovery new therapeutic targets and test drug sensitivities individual tumors. To this end, patient-derived xenograft (PDX) PCa model using immunocompromised mice was established with greater fidelity than is possible currently employed cell lines grown on tissue culture plastic. However, poorly adherent PDX tumor cells exhibit low viability in standard culture,...
Abstract Recently mapped transcriptomic landscapes reveal the extent of heterogeneity in cancer‐associated fibroblasts (CAFs) beyond previously established single‐gene markers. Functional analyses individual CAF subsets within tumor microenvironment are critical to develop more accurate CAF‐targeting therapeutic strategies. However, there is a lack robust preclinical models that reflect this vitro. In study, single‐cell RNA sequencing datasets acquired from head and neck squamous cell...
Chronic inflammation is associated with advanced prostate cancer (PCa), although the mechanisms governing inflammation-mediated PCa progression are not fully understood. progresses to an androgen independent phenotype that incurable. We previously showed independent, receptor negative (AR(-) ) cell lines have high p62/SQSTM1 levels required for survival. also factors in HS-5 bone marrow stromal (BMSC) conditioned medium can upregulate p62 AR(+) lines, leading us investigate AR expression...
Abstract Clinically effective treatments for peritoneal metastases (PM) remain a significant unmet need. To expedite drug development in PM, hyaluronan (HA) hydrogel‐supported PM patient‐derived tumor explants (PDTE) that better preserve histological features, composition, and biological pathways of the original tumor, as compared to conventional PDTE culture methods are developed. Hydrogel modulation shows stiffness, degradation, three‐dimensional embedding, HA itself key parameters enhance...
2657 Background: Current preclinical tumor platforms, such as in vitro 2D cell cultures and organoid models, fail to fully recapitulate the complexity of microenvironment, including critical components like extracellular matrix immune interactions. While humanized patient-derived xenograft models address some these limitations, they are costly, low-throughput, artificial, technically challenging establish. PhaseX (Patient-derived hydrogel-assisted eXplants) presents a robust alternative,...