A5036270463- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Chronic Myeloid Leukemia Treatments
- Kruppel-like factors research
- Acute Myeloid Leukemia Research
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Immunodeficiency and Autoimmune Disorders
- Cytokine Signaling Pathways and Interactions
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- Genomic variations and chromosomal abnormalities
- Skin Protection and Aging
- Acute Lymphoblastic Leukemia research
- Phagocytosis and Immune Regulation
- Infectious Diseases and Mycology
- Cutaneous lymphoproliferative disorders research
- Nonmelanoma Skin Cancer Studies
- Autoimmune Bullous Skin Diseases
- Polydiacetylene-based materials and applications
- Advanced Breast Cancer Therapies
- Urinary and Genital Oncology Studies
Universitat de Vic - Universitat Central de Catalunya
2019-2024
Hospital del Mar Research Institute
2013-2024
Municipal Institute for Medical Research
2008-2021
Hospital Del Mar
2004-2019
Odense University Hospital
2017
Centro de Investigación del Cáncer
2013
Instituto de Investigación Biomédica de Salamanca
2013
Universitat Pompeu Fabra
2013
Institució Catalana de Recerca i Estudis Avançats
2013
Cancer Genetics (United States)
2013
Abstract MicroRNAs (miRNAs) have emerged as promising biomarkers of disease. Their potential use in clinical practice requires standardized protocols with very low miRNA concentrations, particularly plasma samples. Here we tested the most appropriate method for quantification and validated performance a hybridization platform using lower amounts starting RNA. miRNAs isolated from human reference sample were quantified four platforms profiled arrays RNA sequencing (RNA-seq). Our results...
Context: Molecular mechanisms associated with physiological variations in adipose tissue (AT) are not fully recognized. The most recent reports highlight the critical relevance of microRNAs (miRNAs) found AT. Objective: To identify changes messenger RNA (mRNA) and miRNA expressions their interaction human AT before after surgery-induced weight loss. Research Design Setting: Genome-wide mRNA were assessed by microarrays abdominal subcutaneous 16 morbidly obese women 2 years laparoscopic...
Terrestrial ultraviolet (UV) radiation causes erythema, oxidative stress, DNA mutations and skin cancer. Skin can adapt to these adverse effects by repair, apoptosis, keratinization tanning. To investigate the transcriptional response fluorescent solar‐simulated (FSSR) in sun‐sensitive human vivo. Seven healthy male volunteers were exposed 0, 3 6 standard erythemal doses (SED). biopsies taken at h 24 after exposure. Gene microRNA expression quantified with next generation sequencing. A set...
According to current diagnostic criteria, mantle cell lymphoma (MCL) encompasses the usual, aggressive variants and rare, nonnodal cases with monoclonal asymptomatic lymphocytosis, cyclin D1-positive (MALD1). We aimed understand biology behind this clinical heterogeneity identify markers for adequate identification of MALD1 cases.We compared 17 typical MCL a homogeneous group 13 untreated (median follow-up, 71 months). conducted gene expression profiling functional analysis in five MALD1....
The knowledge about chronic spontaneous urticaria (CSU) phenotypes is based on its clinical characteristics, associated comorbidities, course of the disease, and response to available effective drugs. Genotype expression further correlation with CSU are still unknown. We describe cutaneous transcriptome patients suffering a severely active refractory antihistamine treatment.Through bioinformatic analysis whole Human Genome Oligo Microarrays quantitative real-time polymerase chain reaction...
Risk prediction tools cannot identify most individuals at high coronary artery disease (CAD) risk. Oxidized low-density lipoproteins (oxLDLs) and microRNAs are actively involved in atherosclerosis. Our aim was to examine the association of CAD oxLDLs-induced microRNAs, assess predictive capacity future events. Human endothelial vascular smooth muscle cells were treated with oxidized/native lipoproteins, microRNA expression analyzed. Differentially expressed CAD-related miRNAs examined serum...
Molecular mechanisms associated with pathophysiological changes in ventricular remodelling due to myocardial infarction (MI) remain poorly understood. We analyzed gene expression by microarray technology porcine tissue at 1, 4, and 6 weeks post-MI.MI was induced coronary artery ligation 9 female pigs (30-40 kg). Animals were randomly sacrificed or post-MI (n = 3 per group) healthy animals also included as control group. Total RNA from samples hybridized GeneChip® Porcine Genome Arrays....
Background Intestinal metaplasia (IM) is a precursor lesion that precedes gastric cancer (GC). There are two IM histological subtypes, complete (CIM) and incomplete (IIM), the latter having higher progression rates to GC. This study was aimed at analysing gene expression molecular processes involved in from normal mucosa IM, also subtypes Methodology We used data compare transcriptome of healthy not progressing GC, had progressed GC those did progress. Some deregulated genes were validated...
Abstract Although most cutaneous squamous cell carcinomas (cSCCs) develop from actinic keratoses (AKs), the key events in this evolution remain unclear. We have combined results of different genomic and expression array platforms on matched concomitant samples sun‐exposed skin (SES), AK, cSCC 10 immunocompetent patients. Gene analysis copy number alterations were assessed using GeneChip Human 2.0 ST Array (Affymetrix, Santa Clara, CA) CytoScan HD Cytogenetics Solution (Affymetrix) platforms,...
Chronic lymphocytic leukemia (CLL) is characterized by a highly variable clinical course. Del(11q) and del(17p), routinely studied conventional G-banding cytogenetics (CGC) fluorescence in situ hybridization (FISH), have been related to progression shorter overall survival. Recently, array-based karyotyping has gained acceptance as high-resolution new tool for detecting genomic imbalances. The aim of the present study was compare arrays with CGC FISH ascertain whether current techniques...
Abstract: The aim of the present study was to identify genetic aberrations in a series patients with cutaneous large B‐cell lymphoma (LBCL) using comparative genomic hybridization (CGH). Eighteen consecutive primary (13 patients) (PCLBCL) and secondary (five (SCLBCL) were included study. Nine cases corresponded PCLBCL leg type four follicle centre‐cell (PCFCL). Chromosomal imbalances (CIs) detected 14 18 samples (77.8%). All nine two PCFCL showed CIs (100% 50%, respectively). Regarding...
Sarcoptic mange is an emerging and neglected contagious skin disease caused by the mite Sarcoptes scabiei, affecting humans, domestic animals, wildlife. Mange main a major concern for management conservation of populations Iberian ibex (Capra pyrenaica), medium-sized mountain ungulate endemic to Peninsula Northern Pyrenees. Differences in host-parasite interaction host immune response determine clinical outcome, but little known about related differences gene expression. This study...
Several studies in chronic lymphocytic leukemia (CLL) patients have reported impaired immune cell functions, which contribute to tumor evasion and disease progression. However, on CLL-like monoclonal B-cell lymphocytosis (MBL) are scarce. In the study described here, we characterized environment 62 individuals with clinical MBL, 56 early-stage CLL, 31 healthy controls. Gene expression arrays quantitative reverse transcription polymerase chain reaction were performed RNA from CD4+ peripheral...
Abstract Introduction Dysregulated NK cell‐mediated immune responses contribute to tumor evasion in chronic lymphocytic leukemia (CLL), although the cell compartment CLL‐like monoclonal B‐cell lymphocytosis (MBL) is poorly understood. In healthy individuals, human cytomegalovirus (HCMV) induces expansion of cells expressing high levels CD94/NKG2C receptor (NKR) specific for HLA‐E. Methods We analyzed expression NKG2A, NKG2C, ILT2, KIR, CD161, and CD57 24 MBL 37 CLL. NKG2C was genotyped these...
Abstract Deoxyribonucleic acid microarrays allow researchers to measure mRNA levels of thousands genes in a single experiment and could be useful for diagnostic purposes patients with acute myeloid leukaemia (AML). We assessed the feasibility AML profiler (Skyline™ Array) genetic stratification de novo compared results those obtained using standard cytogenetic molecular approach. Diagnostic bone marrow from 31 consecutive cases was used test MLL‐PTD , FLT3‐ITD TKD NPM1 CEBPAdm mutations....
Recent technological advances have made it possible to detect circulating tumor cells (CTCs) as a prognostic marker in operable breast cancer patients. Whether the presence of CTCs patients correlates with molecular alterations primary has not been widely explored. We identified 14 specimens known CTC status, order evaluate differential genetic aberrations by using SNP array assay. There was global increase altered genome, CNA, and copy-neutral loss heterozygosity (cn-LOH) observed...
Abstract Introduction SARS-CoV-2 infection can cause persistent symptoms, which are known as long COVID (LC). The WHO defines this condition the presence of any symptom after viral that persist for at least 3 months. Its incidence is estimated to be between 10% and 70% all cases, include a wide range symptoms. Some them related with cardiorespiratory (CR) systems. identification genetic variants CR symptoms LC could enhance prevention these infection. Purpose To evaluate association LC....
ABSTRACT Muscle-invasive bladder cancer (MIBC) is associated with poor predictability of response to cisplatin-based neoadjuvant chemotherapy (NAC). Consequently, the benefit NAC remains unclear for many patients due lack reliable biomarkers. In order identify biomarkers and build an integrated accurate model predict response, we conducted a comprehensive transcriptomic genomic profiling on 100 MIBC patient tumors. Our analysis identified 602 differentially expressed genes between responders...