A5042895681- Estrogen and related hormone effects
- Cancer Cells and Metastasis
- Breast Cancer Treatment Studies
- Reproductive Biology and Fertility
- AI in cancer detection
- Digital Radiography and Breast Imaging
- Cancer Risks and Factors
- Ovarian function and disorders
- Epigenetics and DNA Methylation
- Cancer Immunotherapy and Biomarkers
- Global Cancer Incidence and Screening
- Cancer Genomics and Diagnostics
- Pluripotent Stem Cells Research
- Chemokine receptors and signaling
- Immune Cell Function and Interaction
- Regulation of Appetite and Obesity
- Immunotherapy and Immune Responses
- Adipokines, Inflammation, and Metabolic Diseases
- Breast Lesions and Carcinomas
- Phytoestrogen effects and research
- Renal and related cancers
- IL-33, ST2, and ILC Pathways
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Effects and risks of endocrine disrupting chemicals
- Infrared Thermography in Medicine
Peter MacCallum Cancer Centre
2015-2024
The University of Melbourne
2015-2024
Monash University
2012-2024
Mayo Clinic in Florida
2023
WinnMed
2023
Cancer Research Institute
2018
Olivia Newton-John Cancer Wellness & Research Centre
2018
Commonwealth Scientific and Industrial Research Organisation
2018
Cancer Research UK Scotland Institute
2015
Prostate Cancer Foundation of Australia
2010
The aromatase-knockout (ArKO) mouse provides a useful model to examine the role that estrogens play in development and homeostasis mammals. Lacking functional Cyp19 gene, which encodes aromatase, ArKO cannot synthesize endogenous estrogens. We examined adipose depots of male female mice, observing these animals progressively accumulate significantly more intraabdominal tissue than their wild-type (WT) littermates, reflected increased adipocyte volume at gonadal infrarenal sites. This...
Accurate estimation of the number ovarian follicles at various stages development is an important indicator process folliculogenesis in relation to endocrine signals and paracrine/autocrine mechanisms that control growth maturation oocytes their supporting follicular cells. There are 10-fold or greater differences numbers per ovary similar ages and/or strains reported earlier studies using methods, leading difficulties with interpretation function vs experimental conditions. This study...
Abstract The translation of basic research into improved therapies for breast cancer patients requires relevant preclinical models that incorporate spontaneous metastasis. We have completed a functional and molecular characterisation new isogenic C57BL/6 mouse model metastasis, comparing contrasting it with the established BALB/c 4T1 model. Metastatic EO771.LMB tumours were derived from poorly metastatic parental EO771 mammary tumours. Functional differences evaluated using both in vitro...
With the development of a mouse model estrogen insufficiency due to targeted disruption aromatase gene [the knockout (ArKO) mouse], new opportunity exists examine role in ovarian follicular development. Ovaries and serum were collected from wild-type, heterozygous, ArKO mice at 10-12 21-23 weeks 1 yr age. The ovaries assessed histologically stereologically, with primary, secondary, antral follicles corpora lutea counted. uteri hypoestrogenic, levels LH FSH females elevated above those...
Proliferation and partial meiotic maturation of germ cells in fetal ovaries is believed to establish a finite, non-renewable pool primordial follicles at birth. The supply postnatal life should be depleted during folliculogenesis, either undergoing atresia or surviving ovulation. Recent studies mouse propose that intra- extraovarian germline stem replenish oocytes form new follicles. We quantified all healthy C57BL/6 from day 1 200 using unbiased stereological methods, immunolabelling oocyte...
Abstract Introduction Mammographic density (MD), after adjustment for a women’s age and body mass index, is strong independent risk factor breast cancer (BC). Although the BC attributable to increased MD significant in healthy women, biological basis of high mammographic (HMD) causation how it raises remain elusive. We assessed histological immunohistochemical differences between matched HMD low (LMD) tissues from women define which cell features may mediate MD-associated risk. Methods...
Male sex, early life chemical exposure and the brain aromatase enzyme have been implicated in autism spectrum disorder (ASD). In Barwon Infant Study birth cohort (n = 1074), higher prenatal maternal bisphenol A (BPA) levels are associated with ASD symptoms at age 2 diagnosis 9 only males low genetic pathway activity scores. Higher BPA predictive of cord blood methylation across CYP19A1 promoter I.f region (P 0.009) gene mediates 0.01) link between brain-derived neurotrophic factor...
Estradiol-17beta (E(2)) affects late follicular development, whereas primordial follicle differentiation and early activation are believed to be independent of E(2). To test this hypothesis we compared numbers primary follicles in wild-type E(2)-deficient, aromatase knockout (ArKO) mice, the immunohistochemical staining or mRNA expression Mullerian inhibiting substance (MIS), Wilms tumor 1 (WT-1), growth factor (GDF9), which known effect differentiation. Proliferating cell nuclear antigen...
Estrogen deficiency in the aromatase knockout (ArKO) mouse leads to development of obesity by as early 3 months age, which is characterized a marked increase weights gonadal and infrarenal fat pads. Humans with natural mutations gene also develop metabolic syndrome. In present study cellular molecular parameters were investigated adipose tissue from 10-wk-old wild-type (WT) ArKO female mice treated 17beta-estradiol or placebo identify basis for intraabdominal obesity. Stereological...
Macrophages play diverse roles in mammary gland development and breast cancer. CC-chemokine ligand 2 (CCL2) is an inflammatory cytokine that recruits macrophages to sites of injury. Although CCL2 has been detected human mouse epithelium, its role regulating cancer risk not explored. Transgenic mice were generated wherein driven by the epithelial cell-specific tumour virus 206 (MMTV) promoter. Estrous cycles tracked adult transgenic non-transgenic FVB mice, glands collected at four different...
The tumor-suppressor PTPN2 is diminished in a subset of triple-negative breast cancers (TNBCs). Paradoxically, PTPN2-deficiency tumors or T cells mice can facilitate cell recruitment and/or activation to promote antitumor immunity. Here, we explored the therapeutic potential targeting tumor and cells. TNBC associated with infiltrates PD-L1 expression, whereas low improved survival. deletion murine mammary epithelial models, did not tumorigenicity but increased STAT-1–dependent expression...
Abstract The mammary gland represents a unique model system to study gene functions in adult stem cells. Mammary cells (MaSCs) can regenerate functional epithelium on transplantation into cleared fat pads. We studied the consequences of distinct genetic modifications MaSCs their repopulation and differentiation ability. reconstitution ductal trees was used as cell selection procedure nearly quantitative lentiviral infection efficiency primary epithelial (MECs) rendered enrichment before...
Polarity coordinates cell movement, differentiation, proliferation and apoptosis to build maintain complex epithelial tissues such as the mammary gland. Loss of polarity deregulation these processes are critical events in malignant progression but precisely how at which stage loss impacts on development tumourigenesis is unclear. Scrib a core regulator tumour suppressor gene however date our understanding function gland has been limited culture transplantation studies lines. Utilizing...