A5048387531- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Kawasaki Disease and Coronary Complications
- Immune responses and vaccinations
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Long-Term Effects of COVID-19
- Respiratory viral infections research
- Immune cells in cancer
- Immunodeficiency and Autoimmune Disorders
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Heparin-Induced Thrombocytopenia and Thrombosis
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Immunology Research
- Child Nutrition and Water Access
- Chronic Lymphocytic Leukemia Research
- Inflammasome and immune disorders
- Protein Degradation and Inhibitors
- Complement system in diseases
- Ubiquitin and proteasome pathways
- Reproductive System and Pregnancy
- HIV-related health complications and treatments
Bambino Gesù Children's Hospital
2021-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2021-2025
Sapienza University of Rome
2018-2022
Istituto Pasteur
2015-2017
Mass SARS-Cov-2 vaccination campaign represents the only strategy to defeat global pandemic we are facing. Immunocompromised patients represent a vulnerable population at high risk of developing severe COVID-19 and thus should be prioritized in programs study vaccine efficacy. Nevertheless, most data on efficacy safety available vaccines derive from trials conducted healthy individuals; hence, studies immunogenicity SARS-CoV2 such populations deeply needed. Here, perform an observational...
Abstract Natural killer (NK) immune cells mediate antibody-dependent cellular cytotoxicity (ADCC) by aggregating FcγRIIIA/CD16, contributing significantly to the therapeutic effect of CD20 monoclonal antibodies (mAb). In this study, we show that CD16 ligation on primary human NK anti-CD20 mAb rituximab or ofatumumab stably impairs spontaneous cytotoxic response attributable cross-tolerance several unrelated NK-activating receptors (including NKG2D, DNAM-1, NKp46, and 2B4). Similar effects...
Adoptive transfer of engineered NK cells, one clinical approaches to fight cancer, is gaining great interest in the last decade. However, development new strategies needed improve efficacy and safety cell-based immunotherapy. cell-mediated recognition lysis tumor cells are strictly dependent on expression ligands for cell-activating receptors NKG2D DNAM-1 cells. Of note, PVR/CD155 Nectin-2/CD112 expressed primarily solid poorly normal tissue Here, we generated human expressing either full...
Natural killer (NK) cell-mediated antibody-dependent cellular cytotoxicity (ADCC), based on the recognition of IgG-opsonized targets by low-affinity receptor for IgG FcγRIIIA/CD16, represents one main mechanisms which therapeutic antibodies (mAbs) mediate their antitumor effects. Besides ADCC, CD16 ligation also results in cytokine production, particular, NK-derived IFNγ is endowed with a well-recognized role shaping adaptive immune responses.Obinutuzumab glycoengineered anti-CD20 mAb...
Although SARS-CoV-2 immunizations have started in most countries, children are not currently included the vaccination programs; thus, it remains crucial to define their anti-SARS-CoV-2 immune response order minimize risk for other epidemic waves. This study sought provide a description of virology ad immunity with distinct symptomatology.Between March and July 2020, we recruited 15 asymptomatic (AS) 51 symptomatic (SY) children, stratified according WHO clinical classification. We measured...
Immunization of vulnerable populations with distinct immunity often results in suboptimal immunogenicity, durability, and efficacy.
Patients affected by Inflammatory Bowel Disease (IBD) present higher risk for infection and suboptimal response upon vaccination. The immunogenicity of SARS-CoV2 vaccination is still largely unknown in adolescents or young adults IBD (pIBD). We investigated the safety BNT162B2 mRNA COVID-19 vaccine 27 pIBD, as compared to 30 healthy controls (HC). Immunogenicity was measured anti-SARS-CoV2 IgG (anti-S anti-trim Ab) before vaccination, after 21 days (T21) 7 second dose (T28). profile close...
Despite the multiple benefits of vaccination, cardiac adverse Events Following COVID-19 Immunization (c-AEFI) have been reported. These events as well severe involvement reported in Multisystem inflammatory syndrome children (MIS-C) appear more frequent young adult males. Herein, we firstly report on profiles patients experiencing c-AEFI comparison with age, pubertal age and gender matched MIS-C involvement. Proteins related to systemic inflammation were found higher compared c-AEFI, whereas...
Natural killer (NK) cells represent a pivotal player of innate anti-tumor immune responses. The impact environmental factors in shaping the representativity different NK cell subsets is increasingly appreciated. Human cytomegalovirus (HCMV) infection profoundly affects compartment, as documented by presence CD94/NKG2C+FcεRIγ- long-lived "memory" subset, endowed with enhanced CD16-dependent functional capabilities, fraction HCMV-seropositive subjects. However, requirements for memory pool...
Multiple Myeloma (MM) is an incurable hematologic malignancy of terminally differentiated plasma cells (PCs), where immune interactions play a key role in the control cancer cell growth and survival. In particular, MM characterized by highly immunosuppressive bone marrow microenvironment anticancer/cytotoxic activity Natural Killer (NK) impaired. This study focused on understanding whether modulation neddylation can regulate NK cell-activating ligands expression sensitize to killing....
Obinutuzumab is a glycoengineered tumor-targeting anti-CD20 mAb with modified crystallizable fragment (Fc) domain designed to increase the affinity for FcγRIIIA/CD16 receptor, which was recently approved clinical use in CLL and follicular lymphoma. Here we extend our previous observation that, human NK cells, sustained CD16 ligation by obinutuzumab-opsonized targets leads markedly enhanced IFN-γ production upon subsequent cytokine re-stimulation. The increased competence response IL-2 or...
Human cytomegalovirus (CMV) is a common herpesvirus causing lifelong latent infection in most people and primary cause of congenital worldwide. Given the role NK cells materno-fetal barrier, we investigated peripheral blood cell behavior context CMV acquired during pregnancy. We analyzed phenotype CD107a surface mobilization on PBMCs from CMV-transmitting non-transmitting mothers newborns with or without infection. showed typical CMV-adaptive cells, characterized by higher levels NKG2C,...
Despite progress, the molecular mechanisms underlying Kawasaki Disease (KD) and intravenous immunoglobulin's (IVIG) ability to mitigate inflammatory process remain poorly understood. To characterize this condition, plasma proteomic profiles, flow cytometry, gene expression of T cell subsets were investigated in longitudinal samples from KD patients compared with two control groups. Systems-level analysis acute phase revealed distinctive features KD, involving mainly Th-1 Th-17 mediators...
In this work our aim was to identify early biomarkers in plasma samples associated with mortality children perinatal HIV treated life, potentially inform intervention targeting vulnerable group. 20/215 (9.3%) HIV, enrolled within 3 months of age died prematurely the first year study, despite ART initiation. Using a propensity score, we selected 40 alive study participants having similar clinical and virological records compared deceased 13 unexposed (HU) healthy were additionally used as...
Most of the current assays directed at investigation HIV reactivation are based on cultures infected cells such as Peripheral Blood Mononuclear Cells (PBMCs) or isolated CD4
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<h3>Background:</h3> Despite progresses, the molecular mechanisms underlying Kawasaki Disease (KD) and intravenous immunoglobulin's (IVIG) ability to mitigate inflammatory process remain incompletely understood. <h3>Objectives:</h3> To comprehensively characterize acute immune profile of KD evaluate how IVIG influence it. <h3>Methods:</h3> In this prospective study, plasma proteomic profile, T cells phenotype, gene-expression sorted cell subsets along with full clinical characteristics, were...