This study further evaluated the in vitro and vivo anti-Helicobacter pylori activities potential underlying mechanism of patchouli alcohol (PA), a tricyclic sesquiterpene. In assay, capacities PA to inhibit kill H. were tested on three standard strains at different pH values 12 clinical isolates. The effects adhesion (and its alpA, alpB, babA genes), motility flaA flaB ultrastructure, flagellation investigated. Moreover, resistance postantibiotic effect (PAE) determined. Furthermore,...

Patchouli alcohol (PA), a natural tricyclic sesquiterpene extracted from Pogostemon cablin (Blanco) Benth. (Labiatae), has been found to exhibit anti-Helicobacter pylori and anti-inflammatory properties. In this study, we investigated the protective effect of PA against H. pylori-induced gastritis in vitro vivo, determined underlying mechanism. vivo experiment, C57BL/6 mouse model was established using SS1, treatments with standard triple therapy or 5, 10 20 mg/kg were performed for 2 weeks....

In this study, we investigated the relaxation effect and mechanisms of patchouli alcohol (PA) on rat corpus cavernosum. Corpus cavernosum strips were used in organ baths for isometric tension studies. The results showed that PA demonstrated concentration-dependent relaxant response to was not influenced by tetrodotoxin atropine while it significantly inhibited removal endothelium. L-NG-nitroarginine methyl ester (L-NAME, a nitric oxide synthase inhibitor) or...

Ablation is one of the main methods for local treatment hepatocellular carcinoma (HCC). Different from radiofrequency ablation (RFA), microwave (MWA) not limited by tissue conductivity, and can use multiple electrodes at same time to improve efficiency. In addition, MWA form a larger area, which makes it possible completely ablate large HCC. However, may be incomplete due factors such as tumors or in high-risk areas. The mechanism cellular tumor immune microenvironment (TIME) involved vitro...

Background: Suo Quan Wan (SQW) is a Chinese traditional prescription that has been used in clinical treatment of lower urinary tract symptoms for centuries.However, scientific basis SQW efficacy and mechanism still needed.This study investigated the effect on bladder function transient receptor potential vanilloid 1 (TRPV1) expression rats with outlet obstruction (BOO).The induced changes overactive (OAB) rat model were observed following different periods to obtain an appropriate...

Abstract Aim Diabetic bladder dysfunction (DBD) is one of the most common and bothersome complications diabetes mellitus (DM). This study aimed to investigate functional, structural, molecular changes at 0, 3, 6, 9, 12 weeks after DM induction by streptozotocin (STZ) in male C57BL/6 mice. Methods Male C57BL/6J mice were injected with STZ (130 mg/kg). Then, diabetic general characteristics, cystometry test, histomorphometry, contractile responses α, β‐methylene ATP, KCl, electrical‐field...

OBJECTIVE: To investigate the effects of Suo Quan Wan (SQW), a traditional Chinese herbal formula, on overactive bladder (OAB) type 2 diabetes mellitus (T2DM) mouse models, particularly its function mediating gene and protein expression levels myosin Va SLC17A9. MATERIALS AND METHODS: After 4 weeks high-fat diet (HFD) feeding, C57BL/6J mice were injected with streptozotocin (100 mg/kg) for four times. 3 weeks, diabetic treated SQW another weeks. Voided stain paper assay, fasting blood...

Suo Quan Wan (SQW) has been used to treat lower urinary tract symptoms (LUTS) in elderly patients for hundreds of years China. β-adrenoceptors (β-ARs), particularly β3-adrenoceptor (β3-AR), was reported be important the bladder dysfunction elderly. The present study conducted explore effect β-AR, and β3-adrenoceptor, aging rat function vitro test therapeutic SQW on LUTS an model based β3-adrenoceptor. Briefly, detrusor muscles young (age, 3 months) 15 female rats were separated. A β-AR...

Suo Quan Wan (SQW) is an effective traditional Chinese prescription on treated lower urinary tract symptoms (LUTS), and has been proved have modulation effect the expression of transient receptor potential vanilloid 1 (TRPV1) in accordance with recovery bladder function overactive rat. This study further investigated mechanism SQW modulated TRPV1 signaling using knockout (KO) mice. Study was conducted wild type KO The animals were grouped into group group. We applied vivo cystometrogram...

This study aimed to elucidate the effects and mechanisms of Radix Linderae (RL) extracts on a mouse model diabetic bladder dysfunction (DBD), especially later decompensated phase. Male C57BL/6J mice were intraperitoneally injected with streptozotocin (STZ) after 4 weeks high-fat diet (HFD) feeding. DBD models (later phase) developed by 12-weeks persistent hyperglycemia then treated RL for weeks. During administration, fasting blood glucose (FBG) test was performed once week. Four later, oral...

Abstract Overactive bladder (OAB) is a common, long-term symptom complex with high prevalence in women worldwide. OAB has caused social burden, and effective treatments are urgently needed. However, the pathogenesis of yet to be elucidated. Model rats underwent outlet obstruction surgery. In 2nd, 3rd, 4th weeks after surgery, metabolic cages were used detect 12 h urine volume sham model groups. The urodynamic parameters leak point pressure (BPLL), maximum voiding (MVP), residual (RV),...

Background Contrast-induced nephropathy (CIN) can lead to serious complications following percutaneous coronary intervention (PCI). Urine N-Acetyl-β-D-glucosaminidase (uNAG) and serum homocysteine (sHCY) are both potential predictors for CIN detection, but their combination has not been explored. We aimed combine uNAG sHCY as the early detection of prognosis prediction in patients after PCI. Methods A total 232 consecutive who underwent PCI at a university hospital were recruited this study....

The lower urinary tract has two main functions, namely, periodic urine storage and micturition; these functions are mediated through central peripheral neuroregulation. Although extensive research on the nervous system been conducted, most studies have focused primary culture. This protocol introduces a method for isolation culture of bladder neurons glia from Sprague-Dawley rats. In this method, were incubated in 37 °C, 5% CO2 incubator 5-7 days. As result, they grew into mature shapes...

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology Pharmacology1 Apr 2014MP17-14 CORRECTION OF HYPERGLYCEMIA AND HYPERINSULINEMIA BY GENETIC MODIFICATION RESTORES BLADDER DYSFUNCTION ASSOCIATED WITH TYPE 2 DIABETES Zongwei Wang, Vivian Cristofaro, Zhiyong Cheng, Hongying Cao, Evgeniy Kreydin, Joseph Gabrielsen, Rongbin Ge, Shulin Wu, Chao Cai, Peng Maryrose Sullivan, Morris White, and Aria Olumi WangZongwei Wang More articles by this author , CristofaroVivian Cristofaro...

You have accessJournal of UrologyBladder & Urethra: Anatomy, Physiology Pharmacology II1 Apr 2016MP30-18 ENHANCED ATP RELEASE AND P2X1R EXPRESSION CONTRIBUTE TO BLADDER DYSFUNCTION IN TYPE 2 DIABETES Zongwei Wang, Vivian Cristofaro, Hongying Cao, Rongbin Ge, Maryrose Sullivian, and Aria Olumi WangZongwei Wang More articles by this author , CristofaroVivian Cristofaro CaoHongying Cao GeRongbin Ge SullivianMaryrose Sullivian OlumiAria View All Author...

Abstract Background Overactive bladder (OAB) is a common, long-term symptom complex with high prevalence in women worldwide. The pathogenesis of OAB has not been elucidated. This study investigated the excitatory purinergic and cholinergic nerve conduction rat model induced by partial outlet obstruction(PBOO）. Methods Model rats underwent obstruction surgery. In 2nd, 3rd, 4th week after surgery, metabolic cages were used to detect 12-hours Urine volume sham groups. urodynamic parameters...

Abnormal bladder nerve conduction was commonly observed in different diabetic models and myosin Va is crucial to neurotransmission. Whether plays a role regulating neurotransmission-mediated overactive (OAB) rats remains unclear. To test this hypothesis, were injected with streptozotocin (STZ) develop diabetes. The function of evaluated by urodynamic test, detrusor tension histological examination at 2, 3, 4 weeks after STZ injection. We found that developed OAB abnormal changes structure At...

The incidence rate of liver cancer has been rising in recent years. Traditional cell line culture and human patient-derived tumor xenograft models, which are commonly used tools to simulate the occurrence cancer, have deepened understanding occurrence, development, drug resistance mechanisms. However, they cannot reflect accurate state cells, microenvironment, or spatial structural characteristics. Recently, more vitro-produced physiological organoids applied study cancer. Liver organoid...