A5101845152- Innovative Microfluidic and Catalytic Techniques Innovation
- Single-cell and spatial transcriptomics
- Biosensors and Analytical Detection
- Traditional Chinese Medicine Analysis
- Microfluidic and Capillary Electrophoresis Applications
- Natural product bioactivities and synthesis
- RNA modifications and cancer
- Soft Robotics and Applications
- High-Voltage Power Transmission Systems
- Circular RNAs in diseases
- Eicosanoids and Hypertension Pharmacology
- Advanced Biosensing Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Analytical chemistry methods development
- Energetic Materials and Combustion
- Ginseng Biological Effects and Applications
- Phytochemistry and Biological Activities
- Microfluidic and Bio-sensing Technologies
- Food Quality and Safety Studies
- Rocket and propulsion systems research
- Cancer-related molecular mechanisms research
- Solar Radiation and Photovoltaics
- Medicinal Plants and Neuroprotection
- Prosthetics and Rehabilitation Robotics
- Dielectric materials and actuators
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2024-2025
Zhejiang University
2007-2025
South China University of Technology
2022-2025
Zhejiang University of Science and Technology
2025
Affiliated Hospital of Shandong University of Traditional Chinese Medicine
2024
Qingdao University
2013-2024
State Key Laboratory of Clean Energy Utilization
2024
Xi'an Jiaotong University
2024
Xinjiang Normal University
2011-2023
Xiangya Hospital Central South University
2023
Abstract The shotgun proteomic analysis is currently the most promising single-cell protein sequencing technology, however its identification level of ~1000 proteins per cell still insufficient for practical applications. Here, we develop a pick-up (PiSPA) workflow to achieve deep capable quantifying up 3000 groups in mammalian using label-free quantitative method. PiSPA specially established samples mainly based on nanoliter-scale microfluidic liquid handling robot, achieving capture,...
Abstract Despite the advantages of digital nucleic acid analysis (DNAA) in terms sensitivity, precision, and resolution, current DNAA methods commonly suffer a limitation multiplexing capacity. To address this issue, droplet encoding‐pairing enabled strategy is developed, wherein unique tricolor combinations are deployed to index individual primer droplets. The template droplets sequentially introduced into microfluidic chip with calabash‐shaped microwell array pairwise trapped merged...
Although single-cell multi-omics technologies are undergoing rapid development, simultaneous transcriptome and proteome analysis of a individual still faces great challenges. Here, we developed (scSTAP) platform based on microfluidics, high-throughput sequencing, mass spectrometry technology to achieve deep joint quantitative at the level, providing an important resource for understanding relationship between transcription translation in cells. This was applied analyze single mouse oocytes...
Summary Salicylic acid ( SA ) plays a critical role in plant defense against pathogen invasion. ‐induced viral plants is distinct from the pathways mediating bacterial and fungal involves specific pathway mediated by mitochondria; however, underlying mechanisms remain largely unknown. The SA‐binding activity of recombinant tomato Solanum lycopersicum alpha‐ketoglutarate dehydrogenase Sl α‐k GDH E2 subunit tricarboxylic (TCA) cycle was characterized. biological this binding defenses tobacco...
Characterizing the profiles of proteome and metabolome at single-cell level is great significance in multiomic studies. Herein, we proposed a novel strategy called one-shot analysis (scPMA) to acquire information individual one injection LC-MS/MS analysis. Based on scPMA strategy, total workflow was developed achieve capture, nanoliter-scale sample pretreatment, LC separation enzyme-digested peptides metabolites, dual-zone MS/MS detection for profiling. Benefiting from realized dual-omic...
The digital nucleic acid detection assay features the capability of absolute quantitation without need for calibration, thereby facilitating rapid identification pathogens. Although several integrated techniques have been developed, there are still constraints in terms automation and analysis throughput. To tackle these challenges, this study presents a digital-to-droplet microfluidic device comprising microfluidics (DMF) module at bottom droplet top. Following sample introduction,...
Protein phosphorylation plays an important role in cellular regulation and signal transduction. In this study, we developed a microamount phosphopeptide enrichment system (mPES) to achieve the of phosphopeptides nanoliter-scale based on microfluidics solid-phase microextraction (SPME) technique. Based mPES, established complete set phosphoproteomic analysis workflow for trace cell samples, including operations capture, lysis, protein proteolysis, enrichment, liquid chromatography-tandem mass...
The aim of this study was to explore the protective effects Salvia miltiorrhiza injection against learning and memory impairment in streptozotocin (STZ)‑induced diabetic rats possible mechanism involved. Sprague Dawley male (n=30) were randomized into three groups: Diabetes, diabetes treated with S. normal control. Diabetes induced by an intraperitoneal STZ (65 mg/kg). injection‑treated received (5 ml/kg/day) while other two groups administered same volume 0.9% saline for four weeks. After...
Abstract It is very essential to grow efficient and abundant photocatalysts for overall water cracking produce hydrogen. Ni 3 FeN nanosheets were synthesized by combining simple sol–gel calcining methods using urea as nitrogen source. A heterostructure was constructed between g‐C N 4 enhance the absorption capacity of visible light. The reformed Z‐scheme FeN/g‐C heterojunction exhibited an excellent visible‐light photocatalytic activity. average hydrogen evolution rate 5 wt% composite 528.7...
In this paper, the infinite-dimensional port-Hamiltonian modelling and control problem of a flexible beam actuated using ionic polymer metal composite (IPMC) actuators is investigated. The framework used to propose an interconnected model mechanical IPMC actuator. dynamic modelled as Timoshenko beam, electric dynamics IPMCs are considered in model. Furthermore, passivity-based control-strategy obtain desired configuration proposed system, closed-loop stability analyzed early lumped approach....
Xiang-Sha-Liu-Jun-Zi-Tang(XSLJZT) is a common formula for the treatment of Gastric Cancer(GC) and widely used in clinical practice, however, there lack investigation into its mechanism.We collected organized drug disease targets, constructed "XSLJZT-Active Ingredient-Target" visualization network, performed GO KEGG functional enrichment analysis crossover genes, followed by molecular docking active ingredients core targets. The best docked monomers were combined with weighted gene...
IRN intervened in AS by modulating glycerophospholipid metabolism, linoleic acid metabolism and alpha-linolenic metabolism.
This design is based on a flexible wire transmission drive system in order to achieve more supple, flexible, lightweight, and cost-effective functionality for the exoskeleton robotic hand. The entire hand equipped with ten wires, power exclusively sourced from single motor (a single-drive configuration). control of motion output this singular source wires five fingers efficiently achieved through operation structure on-off electromagnetic coils, facilitating start-stop function. Furthermore,...