A5074128897- Epigenetics and DNA Methylation
- Prostate Cancer Treatment and Research
- Cancer-related gene regulation
- Cancer Cells and Metastasis
- Autophagy in Disease and Therapy
- PI3K/AKT/mTOR signaling in cancer
- RNA modifications and cancer
- Chemokine receptors and signaling
- Cancer Genomics and Diagnostics
- Histone Deacetylase Inhibitors Research
- Cancer-related Molecular Pathways
- Cytokine Signaling Pathways and Interactions
- Extracellular vesicles in disease
- Immune cells in cancer
- Ubiquitin and proteasome pathways
- Immunotherapy and Immune Responses
- Adenosine and Purinergic Signaling
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Genetic factors in colorectal cancer
- Mechanisms of cancer metastasis
- Advanced Fluorescence Microscopy Techniques
- Erythrocyte Function and Pathophysiology
- RNA Research and Splicing
- Peptidase Inhibition and Analysis
University of Cincinnati
2018-2025
University of Cincinnati Medical Center
2017-2025
Shanghai Jiao Tong University
2010-2017
Ruijin Hospital
2013
Shanghai Public Health Clinical Center
2013
The University of Sydney
2013
Abstract Zn 2+ plays important roles in metabolism and signaling regulation. Subcellular compartmentalization is essential for organelle functions cell biology, but there currently no method to determine relationships among more than two different organelles with one probe. Here, we report simultaneous tracking multiple (Zn-STIMO), a that uses structured illumination microscopy (SIM) single fluorescent probe, allowing super-resolution morphology-correlated identification living cells. To...
Abstract Mitochondria are highly dynamic organelles whose fragmentation by fission is critical to their functional integrity and cellular homeostasis. Here, we develop a method via optogenetic control of mitochondria–lysosome contacts (MLCs) induce mitochondrial with spatiotemporal accuracy. MLCs can be achieved blue-light-induced association mitochondria lysosomes through various photoactivatable dimerizers. Real-time induction tracked in living cells measure the rate. The partially...
// Gang Xiao 1 , Xiumin Wang Jinglong Lidong Zu Guangcun Cheng Mingang Hao Xueqing Sun Yunjing Xue Jinsong Lu 2 and Jianhua 3 Department of Biochemistry Molecular Cell Biology, Shanghai Key Laboratory Tumor Microenvironment Inflammation, Jiao Tong University School Medicine, Shanghai, China Comprehensive Breast Health Center, Renji Hospital, Cancer Institute, Fudan Correspondence to: Wang, email: Lu, Keywords : CXCL16/CXCR6, breast cancer, ERK1/2, RhoA Received January 01, 2015 Accepted...
Tissue inhibitor of metalloproteinases-1 (TIMP-1) is a multifunctional protein that can directly regulate apoptosis and metastasis. In this study, we investigated the functional molecular mechanisms by which TIMP-1 influences triple-negative breast cancer (TNBC). The expression level in tissues was analyzed using ONCOMINE microarray database. overall survival patients with distinct subtypes stratified levels evaluated Kaplan–Meier analysis. Bisulfate sequencing PCR (BSP) used to analyze...
Breast cancer (BrCa) is the malignant tumor that most seriously threatens female health; however, molecular mechanism underlying its progression remains unclear. Here, we found conditional deletion of hypermethylated in 1 (HIC1) mouse mammary gland might contribute to premalignant transformation early stage formation. Moreover, chemokine (C-X-C motif) ligand 14 (CXCL14) secreted by HIC1-deleted BrCa cells bound cognate receptor GPR85 on fibroblasts microenvironment and was responsible for...
Cancer-associated fibroblasts (CAFs) have been implicated in the development of resistance to anticancer drugs; however, role and mechanism underlying CAFs luminal breast cancer (BrCA) tamoxifen are unclear. We found that stromal isolated from central or peripheral area BrCA similar CAF phenotype activity. In vitro vivo experiments showed derived clinical–luminal BrCAs induce through decreasing estrogen receptor-α (ER-α) level when cultured with cell lines MCF7 T47D. promoted interleukin-6...
Breast cancer stem cells (BCSCs) contribute to intra-tumoral heterogeneity and therapeutic resistance. However, the binary concept of universal BCSCs co-existing with bulk tumor is over-simplified. Through single-cell RNA-sequencing, we found that Neu, PyMT BRCA1-null mammary tumors each corresponded a spectrum minimally overlapping cell differentiation states without BCSC population. Instead, our analyses revealed these contained distinct lineage-specific propagating (TPCs) this reflective...
Abstract Prostate cancer (PCa) is a malignant tumor that seriously threatens men’s health worldwide. Recently, stromal cells in the microenvironment (TME) have been reported to contribute progression of PCa. However, role and mechanism how PCa interact with reshape TME remain largely unknown. Here, using spontaneous prostate adenocarcinoma (PRAD) model driven by loss Pten Hic1 , we found M2 macrophages markedly infiltrated stroma double conditional knockout ( dCKO ) mice compared those...
Abstract Purpose: Prostate cancer is the second leading cause of deaths among men in Western counties, which has also occurred Chinese male with markedly increasing incidence recent years. Although mechanism underlying its progression still remains unclear, epigenetic modifications are important ethological parameters. The purpose this study to determine methylation status and function hypermethylatioted 1 (HIC1) prostate progression. Experimental Design: HIC1 promoter was assayed cell...
Increasing evidence shows that micro RNAs (miRNAs) play a critical role in tumor development. However, the of miRNAs non-small cell lung cancer (NSCLC) metastasis remains largely unknown. Here, we found miR-124 expression was significantly impaired NSCLC tissues and associated with its metastasis. In vitro vivo experiments indicate restoring cells had marked effect on reducing migration, invasion Mechanistic analyses show Smad4, cobinding protein transforming growth factor-β (TGF-β) pathway,...
Abstract Lysosome–autophagosome fusion is critical to autophagosome maturation. Although several proteins that regulate this process have been identified, the prefusion architecture and its regulation remain unclear. Herein, we show upon stimulation, multiple lysosomes form clusters around individual autophagosomes, setting stage for membrane fusion. The soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) on lysosomes—vesicle-associated 8 (VAMP8)—plays an important...
Abstract The tumor suppressor gene HIC1 is frequently deleted or epigenetically silenced in human cancer, where its restoration may improve cancer prognosis. Here, we report results illuminating how silencing alters effect signals triple-negative breast (TNBC), which are crucial for pathogenesis. expression was only TNBC compared with other molecular subtypes of cancer. Restoring cells reduced cell migration, invasion, and metastasis, whereas RNAi-mediated untransformed increased their...
Everolimus, an mTOR inhibitor, showed great clinical efficacy in combination with tamoxifen, letrozole, or exemestane for the treatment of estrogen receptor-positive (ER+) breast cancer. However, its antitumor activity was shown to be compromised by a compensatory process involving AKT activation. Here, it determined whether combining additional PI3K inhibitor can reverse this phenomenon and improve efficacy. In cancer cells (MCF-7 BT474), everolimus inhibited downstream limiting...
It is a major challenge to treat metastasis due the presence of heterogenous BCSCs. Therefore, it important identify new molecular targets and their underlying mechanisms in various BCSCs improve treatment breast cancer metastasis. Here, we performed RNA sequencing on two distinct co-existing BCSC populations, ALDH+ CD29hi CD61+ from PyMT mammary tumor cells detected upregulation biglycan (BGN) these Genetic depletion BGN reduced proportions tumorsphere formation. Furthermore, associated...
RB1CC1/FIP200 is an essential macroautophagy/autophagy protein that plays important role in a variety of biological and disease processes through its canonical autophagy-dependent -independent functions. However, it remains largely unknown whether post-translational modifications could regulate RB1CC1 associated autophagy Here, we report acetylation several lysine residues by acetyltransferase CREBBP (CREB binding protein), with K276 as the major site. also identified ubiquitination site...
Abstract HER2 amplification or mutation accounts for 25% of breast cancer patients that can advance to metastatic disease. Therefore, it is important identify novel genes which mediate metastasis in HER2+ cancer. Here, we describe a new suppressor gene, Class II phosphatidylinositol 3-kinases (Pik3c2b), by vivo CRISPR-Cas9 library screening custom designed targeting implicated autophagy using murine (N418) cells. We further showed PI3KC2β KO N418 cells increased their migration and invasion...
Abstract Immune checkpoint inhibitors (ICI) have the potential to induce durable therapeutic responses, yet response rates in breast cancer are modest and limited particular subtypes. To expand applicability of ICI, we examined role an essential autophagy gene, FIP200, which has been shown be important for tumor progression mammary tumors. Specific disruption function FIP200 or complete ablation genetic mouse models revealed that was required PyMT-driven However, a noncanonical responsible...
Autophagy is a conserved cellular process, and its dysfunction implicated in cancer other diseases. Here, we employ an vivo CRISPR screen targeting genes the regulation of autophagy to identify Nsfl1c gene encoding p47 as suppressor human epidermal growth factor receptor 2 (HER2)+ breast metastasis. ablation specifically increases metastasis without promoting primary mammary tumor growth. Analysis patient databases tissue samples indicates correlation lower expression levels with decreased...