A5025233704- Ocean Waves and Remote Sensing
- Oral microbiology and periodontitis research
- Coastal and Marine Dynamics
- Oceanographic and Atmospheric Processes
- Radar Systems and Signal Processing
- Ion Channels and Receptors
- Immune Response and Inflammation
- Endodontics and Root Canal Treatments
- Inflammatory mediators and NSAID effects
- Protease and Inhibitor Mechanisms
- Advanced Sensor and Energy Harvesting Materials
- Aeolian processes and effects
- Advanced Glycation End Products research
- Neurological Disease Mechanisms and Treatments
- Hydrogels: synthesis, properties, applications
- Oral and gingival health research
- Ion channel regulation and function
- GNSS positioning and interference
- Enzyme Production and Characterization
- Underwater Acoustics Research
- Natural Antidiabetic Agents Studies
- Marine Sponges and Natural Products
- NF-κB Signaling Pathways
- Streptococcal Infections and Treatments
- Dental Erosion and Treatment
Kagoshima University
2003-2015
American Association of Endodontists
2010
Kagoshima University Hospital
2009
Tohoku University
1972-2006
Mie University
2003-2005
Kokusai Junior College
1999-2005
Yamagata University Hospital
2004
University at Buffalo, State University of New York
1996-1998
Nara Women's University
1995
National Institute of Infectious Diseases
1991
Lipopolysaccharides (LPS) prepared from Bacteroides intermedius (Prevotella intermedia) and (Porphyromonas) gingivalis by hot phenol-water extraction induced interleukin-8 (IL-8) mRNA in normal human gingival fibroblast cultures, as demonstrated Northern (RNA) blot analysis. IL-8 levels began to increase after a 2-h exposure, reached maximum 12 h, then dropped the unstimulated level at 48 h. were also enhanced dose-dependent manner. By contrast, LPS specimens various Salmonella species with...
ABSTRACT Large numbers of bacteria coexist in the oral cavity. Streptococcus sanguinis , one major dental plaque, produces hydrogen peroxide (H 2 O ), which interferes with growth other bacteria. mutans a cariogenic bacterium, can S. but to do so, it needs means detoxifying H produced by . In this study, we investigated association three oxidative stress factors, Dpr, superoxide dismutase (SOD), and AhpCF, resistance The knockout dpr sod significantly increased susceptibility while ahpCF had...
Cysteine proteases, including Arg-gingipain of Porphyromonas gingivalis, have been implicated as important virulence factors in periodontal diseases. These enzymes are also involved the hemagglutinating activity organisms. In order to determine role proteases colonization gingival margin, we compared attachment properties P. gingivalis 381 with those its Arg-gingipain-defective mutant, G-102. Interactions gram-positive bacteria, human oral epithelial cells, extracellular matrix proteins, and...
In order to access the role of Porphyromonas gingivalis Arg-gingipain proteases in virulence this organism, a mutant defective rgpA gene was constructed strain 381. This mutant, MT10, displayed only 40% Arg-specific cysteine protease activity wild-type strain. addition, as well recently characterized G-102, which is rgpB gene, reduced self-aggregation, hemagglutination, and ability bind immobilized type I collagen compared levels parent. However, unlike increased binding epithelial cells...
The complete nucleotide sequence of the gene for a cell surface protein antigen (SpaA) Streptococcus sobrinus MT3791 (serotype g) was determined. spaA consisted 4,698 bp and coded 170,202 Da. A putative signal peptide found in amino-terminal end protein. potential promoter Shine-Dalgarno preceded open reading frame. Two internal repeating amino acid sequences were present SpaA. One region, located rich alanine, other, central proline. molecular structure SpaA very similar to that mutans.
High mobility group box 1 (HMGB1), a nonhistone DNA-binding protein, is released into the extracellular space and promotes inflammation. HMGB1 binds to related cell signaling transduction receptors, including receptor for advanced glycation end products (RAGE), which actively participate in vascular inflammatory diseases. The aim of this study was examine whether RAGE are involved pathogenesis pulpitis investigate effect Prevotella intermedia (P. intermedia) lipopolysaccharide (LPS) on...
In a previous study, we demonstrated that the amount of interleukin (IL)-8 mRNA expressed by human gingival fibroblasts stimulated with lipopolysaccharide (LPS) from Prevotella intermedia ATCC 25611 is increased pre-treatment beta or gamma interferon (IFN-β --y). present identified regulatory effects these IFNs on IL-8 expression and production fibroblasts. Priming IFN-alpha (a), -β, --y upregulated in response to P. LPS, whereas co-stimulation reduced cells. The regulation induced...
Streptococcus mutans and sobrinus are the major causative organisms of human dental caries. Pulsed-field gel electrophoresis (PFG) showed that restriction enzyme NotI produced ten six DNA fragments from genomes S. strain MT8148 6715, respectively. The sizes chromosomes were each estimated to be about 2200 kb. map genome was constructed by Southern blot analysis with probes overlapped two adjacent fragments. Several virulence-associated genes placed on map. In addition, unique 'fingerprints'...
Abstract Aquaporin 3 (AQP3) is the predominant water channel protein in human keratinocytes and acts as an inflammatory mediator some lesions. A chronic, process of periodontitis related with a dramatic change surrounding fluid homeostasis to plasma extravasation. The exact pattern aquaporin (AQP) expression its mechanism periodontal disease still unknown. We describe herein up‐regulated AQP3 epithelial lesion chronic functional role. levels inflamed gingival tissues were significantly...
A new method for estimating directional wave spectra from the Doppler by HF radar is proposed. This developed introducing a Bayesian approach, previously proposed one of authors (Hashimoto et al., 1987), as most accurate and reliable methods in situ measurements. The principal advantage that it can be applied without empirical weighting coefficients. Applicability, validity accuracy axe demonstrated with numerically simulated data various conditions.
ABSTRACT Although the strong protease activity of Porphyromonas gingivalis appears to be an important virulence property these organisms, little information is currently available regarding regulation expression multiple genes. Utilizing lacZ reporter gene strategy, environmental factors which regulate Arg-gingipain rgpA and prtT were investigated. These two genes are reciprocally regulated since retarded growth (iron depletion nutrient limitation) appeared upregulate while down-regulating...
Substance P (SP) induces the expression of proinflammatory cytokines, such as interleukin (IL)-6, which are implicated in pulp inflammation. To determine signal pathway SP-induced IL-6, we examined activities mitogen-activated protein kinases (MAPKs) human dental cell (PF-10) cultures. SP induced phosphorylation p38 MAPK within 5 min; this activation persisted for up to 40 min and was independent extracellular signal-related (ERK-1 ERK-2) that were after stimulation PF-10 cells. As shown by...