A5008014053- Bone Metabolism and Diseases
- Bone health and treatments
- NF-κB Signaling Pathways
- TGF-β signaling in diseases
- Immune Response and Inflammation
- Bone and Dental Protein Studies
- Heterotopic Ossification and Related Conditions
- Inflammatory mediators and NSAID effects
- Cytokine Signaling Pathways and Interactions
- Cell Adhesion Molecules Research
- Bone health and osteoporosis research
- Metabolism, Diabetes, and Cancer
- Immune Cell Function and Interaction
- Endodontics and Root Canal Treatments
- Fibroblast Growth Factor Research
- Osteomyelitis and Bone Disorders Research
- Ubiquitin and proteasome pathways
- Bone and Joint Diseases
- Mesenchymal stem cell research
- Bone Tissue Engineering Materials
- Wound Healing and Treatments
- Cell death mechanisms and regulation
- Salivary Gland Disorders and Functions
- dental development and anomalies
- Biomarkers in Disease Mechanisms
Kyushu University
1998-2025
Daiichi University of Pharmacy
2023
Kyushu Dental University
2010-2019
BioChem Technology (United States)
2017
Toxicologie, Pharmacologie et Signalisation Cellulaire
2010-2012
In-Q-Tel
2012
Saitama Medical University
2008-2011
Yale University
2000-2010
Fukuoka Dental College
2004-2005
Department of Physiological Sciences
2005
Osteoclast differentiation factor (ODF, also called RANKL/TRANCE/OPGL) stimulates the of osteoclast progenitors monocyte/macrophage lineage into osteoclasts in presence macrophage colony-stimulating (M-CSF, CSF-1). When mouse bone marrow cells were cultured with M-CSF, M-CSF–dependent macrophages (M-BMMφ) appeared within 3 d. Tartrate-resistant acid phosphatase–positive formed when M-BMMφ further for d tumor necrosis α (TNF-α) M-CSF. formation induced by TNF-α was inhibited addition...
Osteoclast differentiation factor (ODF), a novel member of the TNF ligand family, is expressed as membrane-associated protein by osteoblasts/stromal cells. The soluble form ODF (sODF) induces osteoclast precursors into osteoclasts in presence M-CSF. Here, effects sODF on survival, multinucleation, and pit-forming activity murine were examined comparison with those M-CSF IL-1. Osteoclast-like cells (OCLs) formed cocultures osteoblasts bone marrow mRNA RANK (receptor activator NF-kappaB),...
Fibrodysplasia ossificans progressiva (FOP) is a rare autosomal dominant disorder characterized by congenital malformation of the great toes and progressive heterotopic bone formation in muscle tissue. Recently, mutation involving single amino acid substitution morphogenetic protein (BMP) type I receptor, ALK2, was identified patients with FOP. We report here that identical mutation, R206H, observed 19 Japanese sporadic This mutant ALK2(R206H), activates BMP signaling without ligand binding....
We previously reported that interleukin-1 (IL-1) promoted the survival of murine osteoclast-like cells (OCLs) formedin vitro and activated a transcription factor, NF-κB, OCLs. The present study examined whether activation NF-κB is directly involved in OCLs by IL-1. expression IL-1 type I receptor mRNA was detected polymerase chain reaction amplification reverse-transcribed mRNA. An electrophoretic mobility shift assay showed transiently nuclei OCLs, maximal occurred at 30 min. degradation...
Notch signaling plays a key role in various cell differentiation processes including bone homeostasis. However, the specific involvement of regulating osteoclastogenesis is still controversial. In present study, we show that RANKL induces expression Jagged1 and Notch2 marrow macrophages during osteoclast differentiation. Suppression by selective gamma-secretase inhibitor or short hairpin RNA suppresses RANKL-induced osteoclastogenesis. contrast, induction ectopic intracellular enhances...
Bone defects often result from tumor resection, congenital malformation, trauma, fractures, surgery, or periodontitis in dentistry. Although dental implants serve as an effective treatment to recover mouth function tooth defects, many patients do not have the adequate bone volume build implant. The gold standard for reconstruction of large is use autogenous grafts. While graft most clinical method, surgical stress part being extracted and quantity extractable limit this method. Recently...
Smad1, Smad5 and Smad9 (also known as Smad8) are activated by phosphorylation bone morphogenetic protein (BMP)-bound type I receptor kinases. We examined the role of creating constitutively active forms (SmadDVD). Transcriptional activity Smad9DVD was lower than that Smad1DVD or Smad5DVD, even though all three SmadDVDs associated with Smad4 bound to target DNA. The linker region sufficient reduce transcriptional activity. expression increased activation BMP signaling, similar inhibitory...
We have established a method for obtaining an enriched preparation of functionally active osteoclast-like multinucleated cells (enriched OCLs) from co-cultures mouse primary osteoblasts and bone marrow cells. Using these OCLs, the effect osteoblastic on osteoclast function was examined in two assays: pit formation assay actin ring formation. The OCLs cultured 24 h dentine slices formed only few resorption pits. When various numbers were added to areas pits increased proportionally number...
When mouse bone marrow cells were co-cultured with a stromal cell line. ST2, numerous osteoclast-like multinucleated (MNCs) formed. To enrich the MNCs which tartrate-resistant acid phosphatase (TRAP)-positive, we treated cultures dispase. Enriched TRAP-positive adhering to bottom of dish cultured for further 24h medium containing 15% fetal calf serum. More than 80% detached from during culture. However, when ST2 included culture period, survival significantly increased. Moreover, among...
We have established a method for obtaining an enriched preparation of functionally active osteoclast-like multinucleated cells (enriched OCLs) from co-cultures mouse primary osteoblasts and bone marrow cells. Using these OCLs, the effect osteoblastic on osteoclast function was examined in two assays: pit formation assay actin ring formation. The OCLs cultured 24 h dentine slices formed only few resorption pits. When various numbers were added to areas pits increased proportionally number...
Cells from a “knock-in” mouse expressing NF-κB p65 mutant bearing an alanine instead of serine at position 276 (S276A) display significant reduction NF-κB-dependent transcription, even though the forms appropriate complexes that translocate normally to nucleus and bind DNA. Surprisingly, however, expected embryonic lethality hepatocyte apoptosis seen in absence activity, S276A knock-in embryos die different days due variegated developmental abnormalities. We now demonstrate this phenotype is...