A5088865756- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Microfluidic and Bio-sensing Technologies
- Plasmonic and Surface Plasmon Research
- Microfluidic and Capillary Electrophoresis Applications
- Biochemical and Molecular Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Force Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Mitochondrial Function and Pathology
- 3D Printing in Biomedical Research
- Erythrocyte Function and Pathophysiology
- Adenosine and Purinergic Signaling
- Electrowetting and Microfluidic Technologies
- Thermal Radiation and Cooling Technologies
- RNA modifications and cancer
- Polymer Surface Interaction Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- Phagocytosis and Immune Regulation
- Metamaterials and Metasurfaces Applications
- Protein Interaction Studies and Fluorescence Analysis
- Nanopore and Nanochannel Transport Studies
- Optical Coatings and Gratings
National Institute of Neurological Disorders and Stroke
2021-2024
National Institutes of Health
2023
Pennsylvania State University
2012-2022
Park University
2015-2018
Chinese University of Hong Kong
2010-2014
Rapid and homogeneous mixing inside a microfluidic channel is demonstrated via the acoustic streaming phenomenon induced by oscillation of sidewall sharp-edges. By optimizing design sharp-edges, excellent performance fast speed can be achieved in simple device, making our sharp-edge-based micromixer promising candidate for wide variety applications.
Purine biosynthetic enzymes organize into dynamic cellular bodies called purinosomes. Little is known about the spatiotemporal control of these structures. Using super-resolution microscopy, we demonstrated that purinosomes colocalized with mitochondria, and results were supported by isolation purinosome mitochondria. Moreover, number purinosome-containing cells responded to dysregulation mitochondrial function metabolism. To explore role intracellular signaling, performed a kinome screen...
We introduce a novel microfluidic device for cell sorting in continuous flow using tunable standing surface acoustic waves. This method allows individual cells to be precisely directed into five different outlet channels single step. It is versatile, simple, label-free, non-invasive, and highly controllable.
A 3D acoustic tweezers platform is developed to fabricate size-controllable multicellular spheroids in a rapid and high-throughput manner, utilizing the Gor'kov potential field microstreaming.
Significance We show that the assembly/disassembly of purinosome is cell cycle-dependent and correlates with cellular demands for purine biosynthesis encountered during cycle. The number purinosome-containing cells fluctuates: it peaks in G 1 phase HeLa cultured under purine-depleted conditions, but remains high across , S, 2 /M phases fibroblasts incapable salvage owing to a deficiency hypoxanthine-guanine phosphoribosyltransferase. Thus, function biomarker increased metabolic flux through...
We present a novel concept of generating both static and pulsatile chemical gradients using acoustically activated bubbles designed in ladder-like arrangement. Furthermore, by regulating the amplitude bubble oscillation, we demonstrate that gradient profiles can be effectively tuned.
Significance This study draws on the power of superresolution microscopy to investigate how metabolons behave near different subcellular components. We revealed an interdependent relationship among purinosomes, mitochondria, and microtubules. further suggests a role for each in maximizing purine production times high intracellular demand. With increasing number reported metabolons, this has uncovered potential general strategy use networks facilitate metabolic trade between themselves other...
More than a decade of research work in optofluidics has yielded large catalogue optofluidic elements that can manipulate light at the micro-scale (e.g., lenses, prisms). Although these have proven useful for many on-chip processes miniaturized flow cytometry, interferometry and sample spectroscopy), certain deficiencies precluded their use imaging. However, recent imaging avoided entirely focused instead on image capture composition techniques, demonstrating impressive resolution both 2D...
Resolving the temporal dynamics of cell signaling pathways is essential for regulating numerous downstream functions, from gene expression to cellular responses. Mapping these requires exposure cells time-varying chemical signals; are difficult generate and control over a wide range. Herein, we present an acoustofluidic signal generator based on sharp-edge-based micromixing strategy. The device, simply by modulating driving signals acoustic transducer including ON/OFF switching frequency,...
An active, spatiotemporally controllable chemical gradient generator is demonstrated utilizing the acoustic streaming effects induced by acoustically oscillating sharp-edge structures.
Enzymes within the de novo purine biosynthetic pathway spatially organize into dynamic intracellular assemblies called purinosomes. The formation of purinosomes has been correlated with growth conditions resulting in high demand, and therefore, cellular advantage complexation hypothesized to enhance metabolite flux through pathway. However, properties this structure are unclear. Here, we define purinosome a transient expression system as biomolecular condensate using fluorescence microscopy....
Vesicle fusion at preestablished plasma membrane release sites releases transmitters and hormones to mediate fundamental functions like neuronal network activities fight-or-flight responses. This half-a-century-old concept—fusion well-established in excitable cells—needs be modified include the sequential compound reported here—vesicle previously fused Ω-shaped vesicular membrane. With superresolution STED microscopy neuroendocrine chromaffin cells, we real-time visualized pore openings...
Abstract Membrane budding entails forces to transform flat membrane into vesicles essential for cell survival. Accumulated studies have identified coat-proteins (e.g., clathrin) as potential factors. However, mediating many non-coated buddings remain unclear. By visualizing proteins in endocytic live neuroendocrine cells, performing vitro protein reconstitution and physical modeling, we discovered how non-coated-membrane is mediated: actin filaments dynamin generate a pulling force...
The angle resolved surface enhanced Raman scattering (SERS) on two-dimensional Ag hole arrays has been studied as a function of size. enhancement factor found to increase with increasing In particular, by correlating the mappings dispersion relations, attributed fast plasmon polariton radiative decay rate and strong coupling efficiency. Our results indicate that it is possible optimize geometry obtain desirable SERS.
In this article, we present a simple, rapid prototyped polystyrene-based microfluidic device with three-dimensional (3D) interconnected microporous walls for long term perfusion cell culture. Patterned 3D structures were created by chemical treatment together protective mask and the native hydrophobic nature of selectively made hydrophilic using oxygen plasma mask. Using culture device, successfully demonstrated support four days hepatocytes (C3A cells).