A5046147086- Gold and Silver Nanoparticles Synthesis and Applications
- Probiotics and Fermented Foods
- Advanced biosensing and bioanalysis techniques
- Carbon and Quantum Dots Applications
- RNA Research and Splicing
- Spectroscopy Techniques in Biomedical and Chemical Research
- Protein Hydrolysis and Bioactive Peptides
- Luminescence and Fluorescent Materials
- Photochemistry and Electron Transfer Studies
- Free Radicals and Antioxidants
- Radical Photochemical Reactions
- Protein Interaction Studies and Fluorescence Analysis
- Photochromic and Fluorescence Chemistry
- RNA modifications and cancer
- Nanocluster Synthesis and Applications
- Fish Biology and Ecology Studies
- Luminescence Properties of Advanced Materials
- SARS-CoV-2 and COVID-19 Research
- Radiation Detection and Scintillator Technologies
- Ichthyology and Marine Biology
- Force Microscopy Techniques and Applications
- Biosensors and Analytical Detection
- Microbial Metabolites in Food Biotechnology
- Molecular Junctions and Nanostructures
- Phytochemical Studies and Bioactivities
University of Hong Kong
2014-2025
Hong Kong University of Science and Technology
2020-2025
Guangxi University
2016-2025
Jiangxi Academy of Agricultural Sciences
2023-2024
HKUST Shenzhen Research Institute
2020-2023
Guilin Medical University
2020-2022
South China Agricultural University
2021
Ministry of Agriculture and Rural Affairs
2021
Institute of Zoology
2016-2020
Chinese Academy of Sciences
2016-2020
Abstract Surface-enhanced Raman spectroscopy (SERS) has emerged as a powerful tool to detect biomolecules in aqueous environments. However, it is challenging identify protein structures at low concentrations, especially for the proteins existing an equilibrium mixture of various conformations. Here, we develop situ optical tweezers-coupled visualize and control hotspot between two Ag nanoparticle-coated silica beads, generating tunable reproducible SERS enhancements with single-molecule...
Liquid-liquid phase separation (LLPS) is an emerging and universal mechanism for intracellular organization, particularly, by forming membraneless organelles (MLOs) hosting intrinsically disordered proteins (IDPs) as scaffolds. Genetic engineering generally applied to reconstruct IDPs harboring over 100 amino acid residues. Here, we report the first design of synthetic hybrids consisting short oligopeptides fewer than 10 residues "stickers" dextran a "spacer" recapitulate characteristics...
Many intracellular membraneless organelles (MLOs) appear to adapt a hierarchical multicompartment organization for efficient coordination of highly complex reaction networks. Recapitulating such an internal architecture in biomimetic platforms is, therefore, important step facilitate the functional understanding MLOs and enable design advanced microreactors. Herein, we present modular bottom-up approach building synthetic multiphasic condensates using set engineered multivalent...
Abstract RNA helicase DDX21 plays vital roles in ribosomal biogenesis, transcription, and the regulation of host innate immunity during virus infection. How recognizes unwinds how interacts with remain poorly understood. Here, crystal structures human determined three distinct states are reported, including apo‐state, AMPPNP plus single‐stranded (ssRNA) bound pre‐hydrolysis state, ADP‐bound post‐hydrolysis revealing an open to closed conformational change upon binding unwinding. The core...
Reusing nanoporous Ag sheets 20 times after simple ultrasonic cleaning for sensitive SERS detection of organochlorine pesticides.
In recent decades, the sustainability, availability and low toxicity of biomass polyols have attracted more attention. Biomass were used to prepare polyfunctional polyurethane acrylates, which can effectively improve green performance UV-curable coatings. Herein, a multifunctional acrylate containing variety was synthesized characterized. Firstly, itaconic acid (IA), ricinoleic (RA) oleic (OA) reacted with glycidyl methacrylate (GMA) respectively obtain (IAG), (RAG) (OAG). Determination...
SignificanceSonic Hedgehog (Shh) is a key signaling molecule that plays important roles in embryonic patterning, cell differentiation, and organ development. Although fundamentally important, the molecular mechanisms regulate secretion of newly synthesized Shh are still unclear. Our study reveals role for cargo receptor, SURF4, facilitating export from endoplasmic reticulum (ER) via ER signal. In addition, our provides evidence suggesting proteoglycans promote dissociation SURF4 at Golgi,...
It is challenging to characterize single or a few biomolecules in physiological milieus without excluding the influences of surrounding environment. Here we utilize optical plasmonic trapping construct dynamic nanocavity, which reduces diffraction-limited detection volume and provides reproducible electromagnetic field enhancements achieve high-throughput single-molecule surface-enhanced Raman spectroscopy (SERS) characterizations aqueous environments. Specifically, study human Islet Amyloid...
Abstract Membraneless organelles (MLOs) formed via protein phase separation have great implications for both physiological and pathological processes. However, the inability to precisely control bioactivities of MLOs has hindered our understanding their roles in biology, not mention translational applications. Here, by combining intrinsically disordered domains such as RGG mussel-foot proteins, we create an cellulo system, which various biological activities can be introduced metal-mediated...
In this study, functional lactic acid bacteria (LAB) specific for vegetable fermentation were screened from Chinese sauerkraut brine by designing purpose media. The selected LAB strains showed excellent tolerance to high concentrations of bile salt and acids. They can also survive passage through the simulated gastric fluid may be able reach intestine. Through performance measurements fermentation, one these strains, NCU116 was found have best ability, further subjected a series analyses...
Abstract Z-DNA binding protein 1 (ZBP1) is a crucial player in the intracellular recognition of Z-form nucleic acids (Z-NAs) through its Zαβ domain, initiating downstream interactions with RIPK1 and RIPK3 via RHIM domains. This engagement leads to assembly PANoptosomes, ultimately inducing programmed cell death curb pathogen dissemination. How domain cooperate trigger Z-NAs signal transduction remains unclear. Here, we show that ZBP1 condensate formation facilitates antiviral transduction....