A5087403063- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Genetic and Kidney Cyst Diseases
- Lipid Membrane Structure and Behavior
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Electron Spin Resonance Studies
- Photosynthetic Processes and Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Algal biology and biofuel production
- Nuclear Structure and Function
- Advanced Clustering Algorithms Research
- Microtubule and mitosis dynamics
- Long-Term Effects of COVID-19
- Advanced biosensing and bioanalysis techniques
- Geotechnical Engineering and Soil Stabilization
- Acoustic Wave Resonator Technologies
- Biochemical and Structural Characterization
- Wnt/β-catenin signaling in development and cancer
- RNA regulation and disease
- Landslides and related hazards
- Gold and Silver Nanoparticles Synthesis and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Cloud Computing and Resource Management
- Advanced Electron Microscopy Techniques and Applications
University of Hong Kong
2017-2024
Anhui Normal University
2022-2024
Hong Kong University of Science and Technology
2017-2023
United States Air Force Academy
2010-2012
Wuhan University
2004-2012
PLA Army Service Academy
2012
DuPont (United States)
1993-1994
Experimental Station
1994
Yale University
1993
Princeton University
1991
Abstract Single-molecule localization microscopy (SMLM) can be used to resolve subcellular structures and achieve a tenfold improvement in spatial resolution compared that obtained by conventional fluorescence microscopy. However, the separation of single-molecule events requires thousands frames dramatically increases image acquisition time phototoxicity, impeding observation instantaneous intracellular dynamics. Here we develop deep-learning based single-frame super-resolution (SFSRM)...
Significance In the early secretory pathway, newly synthesized proteins undergo folding and modifications then leave ER through COPII-coated vesicles. How these processes are coordinated maintained important but mostly unclear. We show here that ATL, a GTPase connects tubules, controls protein mobility regulates cargo packaging coat assembly of COPII The tethering fusion activity by ATL likely maintains tension other necessary parameters for formation in membranes. These findings reveal role...
The molecular oxygen in our atmosphere is a product of water-splitting reaction that occurs the oxygen-evolving complex photosystem II oxygenic photosynthesis. catalytic core an ensemble four manganese atoms arranged cluster undetermined structure. pulsed electron paramagnetic resonance (EPR) technique spin-echo envelope modulation (ESEEM) can be used to measure nuclear spin transitions nuclei magnetically coupled metal centers enzymes. We report results ESEEM experiments on cyanobacterium...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSpectroscopic evidence from site-directed mutants of Synechocystis PCC6803 in favor a close interaction between histidine 189 and redox-active tyrosine 160, both polypeptide D2 the photosystem II reaction centerXiao Song Tang, Dexter A. Chisholm, G. Charles Dismukes, Gary W. Brudvig, Bruce DinerCite this: Biochemistry 1993, 32, 49, 13742–13748Publication Date (Print):December 14, 1993Publication History Published online1 May 2002Published inissue...
Significance Protein sorting in the secretory pathway is a fundamentally important cellular process, but clients of specific cargo machinery remains largely underinvestigated. Here, utilizing vesicle formation assay to profile proteins associated with vesicles, we identified cytosolic that are membranes GTP-dependent manner or interact GTP-bound Sar1A. We found two them, FAM84B and PRRC1, regulate anterograde trafficking. Moreover, revealed export adaptors, SURF4 ERGIC53. These analyses...
SignificanceSonic Hedgehog (Shh) is a key signaling molecule that plays important roles in embryonic patterning, cell differentiation, and organ development. Although fundamentally important, the molecular mechanisms regulate secretion of newly synthesized Shh are still unclear. Our study reveals role for cargo receptor, SURF4, facilitating export from endoplasmic reticulum (ER) via ER signal. In addition, our provides evidence suggesting proteoglycans promote dissociation SURF4 at Golgi,...
Aimed to the defects of traditional hospital management system, this paper develops a new intelligent system based on cloud computing. The characteristics computing are listed by introducing definition, framework and basic capabilities. It can closely link various medical departments coordinate among institutions in business. With achieving strong information technology resources optimizing patient flow, improves efficiency use resources.
The neurological symptoms of long COVID and viral neuroinvasion have raised concerns about the potential interactions between SARS-CoV-2 protein segments neuronal proteins, which might confer a risk post-infection neurodegeneration, but underlying mechanisms remain unclear. Here, we reported that receptor-binding domain (RBD) spike nine-residue segment (SK9) envelope could bind to α-synuclein (αSyn) with Kd values 503 ± 24 nM 12.7 1.6 μM, respectively. RBD inhibit αSyn fibrillization by...
Planar cell polarity (PCP) is a process during which cells are polarized along the plane of epithelium and regulated by several transmembrane signaling proteins. After their synthesis, these PCP proteins delivered secretory transport pathway to plasma membrane, where they perform physiological functions. However, molecular mechanisms that regulate protein remain largely unclear. Here, we found delivery protein, Frizzled-6, surface two conserved polybasic motifs: one located in its first...
Dynamic light scattering (DLS) is used to monitor the formation of truncated ACE2 peptide-conjugated gold nanoparticles and determine their binding constants kinetics SARS-CoV-2 RBD.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMonitoring oxygen concentration in solution by ESR oximetry using lithium phthalocyanine: application to photosynthesisX. S. Tang, M. Moussavi, and G. C. DismukesCite this: J. Am. Chem. Soc. 1991, 113, 15, 5914–5915Publication Date (Print):July 1, 1991Publication History Published online1 May 2002Published inissue 1 July 1991https://pubs.acs.org/doi/10.1021/ja00015a085https://doi.org/10.1021/ja00015a085research-articleACS PublicationsRequest reuse...
Abstract Single-molecule localization microscopy (SMLM) can be used to resolve subcellular structures and achieve a tenfold improvement in spatial resolution compared that obtained by conventional fluorescence microscopy. However, the separation of single-molecule events thousands frames dramatically increases image acquisition time phototoxicity, impeding observation instantaneous intracellular dynamics. Based on deep learning networks, we develop single-frame super-resolution (SFSRM)...
In the conventional secretory pathway, cargo receptors play important roles in exporting newly synthesized proteins from endoplasmic reticulum (ER). We previously showed that a receptor, surfeit locus protein 4 (SURF4), promotes ER export of soluble signaling molecule, sonic hedgehog, via recognizing polybasic residues within its Cardin-Weintraub motif. addition to we found 30 more containing motif (K/R)(K/R)(K/R)XX(K/R)(K/R), but whether SURF4 plays general role mediating these is unclear....
Protein sorting at the trans Golgi network (TGN) plays important roles in targeting newly synthesized proteins to their specific destinations. The aim of this proposal is reconstitute packaging non-Golgi resident cargo into vesicles TGN, utilizing rat liver cytosol, semi-intact mammalian cells and nucleotides. protocol describes how perform vesicle formation assay, isolate detect vesicles. This reconstitution assay can be used quantitatively measure efficiency a protein transport TGN under...
The failure of slope is a gradual accumulation process. Under the effect many interior and exterior factors, some parts in reach yield with increase stress; sliding surface forms gradually till complete transfixion; plastic strain continuous increases, overall happens on slope. Traditional analysis method cannot display mechanic conditions whole process deformation, transfixion surfaces failure. Meanwhile, FEM strength reduction can quantitatively show deformation features occurrence...
This paper describes the method and realization of objective binary segmented image to obtain goal characteristic quantities. The target block is formed by pixel labeling. Through using mathematical morphology processing filter out noise, it achieves a clear extracting boundary. With license plate character recognition, experiment shows that algorithm effective. All numerical examinations illustrate high convergence speed prove validity recognition rate.
Differential evolution algorithm is a kind of heuristic random search algorithm, and the traditional sample learning to find inductive assertion including all positive examples but not counter-examples in example space. But this process endless cumbersome because large number samples. The merit difference searching community. So paper using combine with then promoting efficiency.
Abstract The fidelity of protein transport in the secretory pathway relies on accurate sorting proteins to their correct destination. To deepen our understanding underlying molecular mechanisms, it is important develop a robust approach systematically reveal cargo that depend specific machinery be efficiently packaged into vesicles. Here, we used an vitro assay reconstitutes packaging human vesicles quantify capture. Quantitative mass spectrometry analyses isolated revealed novel cytosolic...