A5010992487- Gold and Silver Nanoparticles Synthesis and Applications
- Polymer-Based Agricultural Enhancements
- Protein Interaction Studies and Fluorescence Analysis
- Spectroscopy Techniques in Biomedical and Chemical Research
- SARS-CoV-2 and COVID-19 Research
- Advanced biosensing and bioanalysis techniques
- Advanced Nanomaterials in Catalysis
- Computational Drug Discovery Methods
- Laser-Ablation Synthesis of Nanoparticles
- Alzheimer's disease research and treatments
- Nanoporous metals and alloys
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Nanoparticle-Based Drug Delivery
- Hedgehog Signaling Pathway Studies
- Protein Structure and Dynamics
- Nanocomposite Films for Food Packaging
- Neuroscience and Neural Engineering
- Cholinesterase and Neurodegenerative Diseases
- SARS-CoV-2 detection and testing
- Long-Term Effects of COVID-19
- Advanced Electron Microscopy Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Developmental Biology and Gene Regulation
- Biosensors and Analytical Detection
- Hydrogels: synthesis, properties, applications
University of Hong Kong
2020-2025
Hong Kong University of Science and Technology
2020-2025
De La Salle University
2019-2020
Abstract Surface-enhanced Raman spectroscopy (SERS) has emerged as a powerful tool to detect biomolecules in aqueous environments. However, it is challenging identify protein structures at low concentrations, especially for the proteins existing an equilibrium mixture of various conformations. Here, we develop situ optical tweezers-coupled visualize and control hotspot between two Ag nanoparticle-coated silica beads, generating tunable reproducible SERS enhancements with single-molecule...
SignificanceSonic Hedgehog (Shh) is a key signaling molecule that plays important roles in embryonic patterning, cell differentiation, and organ development. Although fundamentally important, the molecular mechanisms regulate secretion of newly synthesized Shh are still unclear. Our study reveals role for cargo receptor, SURF4, facilitating export from endoplasmic reticulum (ER) via ER signal. In addition, our provides evidence suggesting proteoglycans promote dissociation SURF4 at Golgi,...
It is challenging to characterize single or a few biomolecules in physiological milieus without excluding the influences of surrounding environment. Here we utilize optical plasmonic trapping construct dynamic nanocavity, which reduces diffraction-limited detection volume and provides reproducible electromagnetic field enhancements achieve high-throughput single-molecule surface-enhanced Raman spectroscopy (SERS) characterizations aqueous environments. Specifically, study human Islet Amyloid...
Reusing nanoporous Ag sheets 20 times after simple ultrasonic cleaning for sensitive SERS detection of organochlorine pesticides.
The neurological symptoms of long COVID and viral neuroinvasion have raised concerns about the potential interactions between SARS-CoV-2 protein segments neuronal proteins, which might confer a risk post-infection neurodegeneration, but underlying mechanisms remain unclear. Here, we reported that receptor-binding domain (RBD) spike nine-residue segment (SK9) envelope could bind to α-synuclein (αSyn) with Kd values 503 ± 24 nM 12.7 1.6 μM, respectively. RBD inhibit αSyn fibrillization by...
Abstract Membrane environments affect protein structures and functions through protein-membrane interactions in a wide range of important biological processes. To better study the effects from lipid’s hydrophilic hydrophobic interaction with on different membrane regions, we developed lipid-coated nanoporous silver sheets to provide tunable supported lipid monolayer/bilayer for situ surface-enhanced Raman vibrational spectroscopy (SERS) characterizations. Under controllable surface pressure,...
A coated nitrogen-phosphorus-potassium (NPK) fertilizer was prepared via crosslinking chitosan and alginate using citric acid.Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), particle size analysis, zeta potential measurement showed the successful crosslinking, appropriate size, colloidal stability of NPK fertilizer.The encapsulation capability crosslinked chitosan/alginate (Chi/Alg) studied fluorescence spectroscopy analysis.The release behavior studies...
Dynamic light scattering (DLS) is used to monitor the formation of truncated ACE2 peptide-conjugated gold nanoparticles and determine their binding constants kinetics SARS-CoV-2 RBD.
An encapsulant system was prepared via crosslinking of chitosan using citric acid as a crosslinker. FTIR spectroscopy, particle size and zeta potential analyses confirmed successful crosslinking, appropriate colloidal stability. Encapsulation capability the said material investigated fluorescence spectroscopy. The effect pH on crosslinked particles also conducted.
Abstract Amyloidosis is characterized by the abnormal accumulation of misfolded proteins, called amyloid fibrils, leading to diverse clinical manifestations. Recent studies on amyloidogenesis SARS‐CoV‐2 protein segments have raised concerns their potential link post‐infection neurodegeneration, however, mechanisms remain unclear. Herein, we investigated structure, stability, and amyloidogenic propensity a nine‐residue segment (SK9) envelope impact neuronal α‐synuclein (αSyn) aggregation....
This work described the successful preparation of encapsulated nitrogen-phosphorus-potassium (NPK) fertilizers with use carboxymethyl cellulose/alginate (CMC/Alg) blend employing citric acid (CA) as crosslinking agent. The study involved preparation, characterization, release studies, and efficacy evaluation said fertilizer system. Fourier transform infrared (FTIR) fluorescence spectroscopic methods, scanning electron microscopy (SEM), particle size analysis, zeta potential measurements...
A carboxymethylcellulose-based encapsulant system for the controlled release of nitrogen-phosphorus-potassium (NPK) fertilizer was synthesized using alginate as a stabilizer, and citric acid crosslinking agent. Fourier-transform infrared (FT-IR) spectroscopy, particle size analysis, zeta potential measurement, scanning electron microscopy showed successful crosslinking, sufficient colloidal stability, well topography formed particles. Fluorescence spectroscopy confirmed encapsulation model...
Abstract This is a protocol to fabricate silver nanoparticle coated silica beads for optical trapping and surface enhanced Raman spectroscopy (SERS) measurement.
Abstract Surface-enhanced Raman spectroscopy (SERS) is a powerful tool for single-molecule studies. However, it challenging to characterize biomacromolecules with conformational complexity in solutions, concerning its credibility, sensitivity, repeatability, and accuracy, due the limited molecular control of existing SERS platforms. Here, we integrate dual-trap optical tweezers nanoparticle-deposited nanostages typical spectro-microscope correlate manipulations site-specified...
Abstract It is challenging to characterize single or a few biomolecules in physiological milieus without excluding the influences of surrounding molecules and micro-environments. Here we utilize optical plasmonic trapping construct dynamic nanocavity, which reduces diffraction-limited detection volume provides reproducible electromagnetic field enhancements for high-throughput single-molecule surface-enhanced Raman spectroscopy (SERS) characterizations crowded aqueous environments....