A vaccine is needed to combat the Chlamydia epidemic. Replication-deficient viral vectors are safe and induce antigen-specific T-cell memory. We tested ability of intramuscular immunization with modified vaccinia Ankara (MVA) virus or chimpanzee adenovirus (ChAd) expressing chlamydial outer membrane protein (OmcB) secreted protein, protease-like activating factor (CPAF), enhance immunity protection in mice previously infected plasmid-deficient muridarum CM972 elicit naïve mice. MVA.OmcB...

Introduction Resurgence of pertussis, caused by Bordetella necessitates novel vaccines and vaccination strategies to combat this disease. Alum-adjuvanted acellular pertussis (aPV) delivered intramuscularly reduce bacterial numbers in the lungs immunized animals humans, but do not nasal colonization. Thus, aPV-immunized individuals are sources community transmission. We showed previously that modification a commercial aPV (Boostrix) addition Th1/17 polarizing adjuvant Colonization Factor A...

Chlamydia trachomatis (CT) is a globally prevalent sexually transmitted infection that can result in pelvic inflammatory disease, ectopic pregnancy, and infertility women. Currently, there no prophylactic vaccine. This study examined T-cell immunity cohort of women recently infected with CT. Participants were screened against peptides spanning 33 894 possible CT proteins, either ex vivo or using short-term cell lines. CT-specific T cells characterized by interferon (IFN) γ enzyme-linked...

Performance of a 16S rRNA analysis the cervicovaginal microbiome 220 participants recruited into T Cell Response against Chlamydia (TRAC) cohort between February 2011 and August 2014 in Allegheny County, Pennsylvania USA detected DNA encoding chlamydial samples from seven whose tests were negative for trachomatis (CT) gonococcal five Neisseria gonorrhoeae (NG) infection with Aptima Combo 2 assay (Hologic). We used targeted PCR amplification followed by sequencing to characterize 23S locus...

Despite high global vaccine coverage, whooping cough, also known as pertussis, caused by the gram-negative obligate human pathogen Bordetella pertussis (Bp), is resurging worldwide.The inactivated whole cell vaccines (wPV), introduced in 1940s, were extremely effective preventing severe disease, controlling bacterial burden entire respiratory tract, and transmission.Because of reactogenicity negative public perceptions regarding safety, wPV discontinued acellular subunit (1 to 5 protein...

Chlamydia trachomatis (CT) is the most common bacterial sexually transmitted infection globally. Understanding natural immunity to CT will inform vaccine design. This study aimed profile immune cells and associated functional features in CT-infected women, determine profiles with reduced risk of ascended endometrial reinfection. PBMCs from CT-exposed women were profiled by mass cytometry random forest models identified key that distinguish outcomes. CT+ participants exhibited higher...

Infections and disease caused by the obligate human pathogen Bordetella pertussis (Bp) are increasing, despite widespread vaccinations. The current acellular vaccines remain ineffective against nasopharyngeal colonization, carriage, transmission. In this work, we tested hypothesis that polysaccharide (Bps), a member of poly-β-1,6-N-acetyl-D-glucosamine (PNAG/PGA) family polysaccharides promotes respiratory tract colonization Bp resisting killing antimicrobial peptides (AMPs). Genetic...

Chlamydia trachomatis (Ct) is the most common bacterial sexually transmitted infection globally, and a vaccine urgently needed to stop transmission disease. Chlamydial Protease Activity Factor (CPAF) an immunoprevalent immunodominant antigen for CD4 T cells B cells, which makes it strong candidate. Due tolerogenic nature of female genital tract (FGT) its lack secondary lymphoid tissue, effective induction protective cell-mediated immunity will likely require potent safe mucosal adjuvants. To...

ABSTRACT Chlamydia trachomatis (CT) is the most common bacterial sexually transmitted infection globally. Understanding natural immunity to CT will inform vaccine design. This study aimed profile immune cells and associated functional features in CT-infected women, determine profiles with reduced risk of ascended endometrial reinfection. PBMCs from CT-exposed women were profiled by mass cytometry random forest models identified key that distinguish outcomes. CT+ participants exhibited higher...

Abstract Chlamydia trachomatis (CT) is the most common bacterial sexually transmitted infection (STI) in U.S. despite effective antibiotics. Understanding natural immunity to CT crucial inform vaccine design. This study aimed identify immune cell populations and functional features associated with reduced risk of reinfection. PBMCs were collected from 82 CT-exposed women tested for other STIs upon enrollment, repeatedly over 1 year follow-up. Immune responses profiled by mass cytometry....

Performance of a 16S rRNA analysis the cervicovaginal microbiome 220 participants recruited into T Cell Response against Chlamydia (TRAC) cohort between February 2011 and August 2014 in Allegheny County, Pennsylvania USA surprisingly detected DNA encoding chlamydial samples from seven who had tested negatively for

Introduction: Chlamydia trachomatis (CT) infection can lead to pelvic inflammatory disease, infertility and other reproductive sequelae when it ascends the upper genital tract. Factors including chlamydial burden, co-infection with sexually-transmitted bacterial pathogens oral contraceptive use influence risk for tract spread. Cervicovaginal microbiome composition influences CT susceptibility we investigated if contributes spread by analyzing amplicon sequence variants (ASVs) derived from V4...

Bordetella bronchiseptica is an etiologic agent of respiratory diseases in animals and humans. Despite the widespread use veterinary B. vaccines, there limited information on their composition relative efficacy immune responses that they elicit. Furthermore, human vaccines are not available. We leveraged dual antigenic adjuvant functions colonization factor A (BcfA) to develop acellular absence additional adjuvant.

Vaccines are a 20th century medical marvel. They have dramatically reduced the morbidity and mortality caused by infectious diseases contributed to striking increase in life expectancy around globe. Nonetheless, determining vaccine efficacy remains challenge. Emerging evidence suggests that current acellular (aPV) for Bordetella pertussis (B. pertussis) induces suboptimal immunity. Therefore, major challenge is designing next-generation protective immunity without adverse side effects of...

(Ct) is the most common bacterial sexually transmitted infection globally, and a vaccine urgently needed to stop transmission disease. Chlamydial Protease Activity Factor (CPAF) an immunoprevalent immunodominant antigen for CD4 T cells B cells, which makes it strong candidate. Due tolerogenic nature of female genital tract (FGT) its lack secondary lymphoid tissue, effective induction protective cell-mediated immunity will likely require potent safe mucosal adjuvants. To address this need, we...

Abstract Infections and disease caused by the obligate human pathogen Bordetella pertussis (Bp) are increasing, despite widespread vaccinations. The current acellular vaccines remain ineffective against nasopharyngeal colonization, carriage, transmission. In this work, we tested hypothesis that polysaccharide (Bps), a member of poly-β-1,6- A -acetyl-D-glucosamine (PNAG/PGA) family polysaccharides promotes respiratory tract colonization Bp resisting killing antimicrobial peptides (AMPs)....

Objectives Identify genetic loci of enhanced susceptibility to Chlamydial trachomatis (Ct) upper genital tract infection in women. Methods We performed an integrated analysis DNA genotypes and blood-derived mRNA profiles from 200 Ct- exposed women identify expression quantitative trait (eQTL) determine their association with endometrial chlamydial using a mediation test. further evaluated the effect lead eQTL on CD151 by immune cells associated low high whole blood , respectively. Results...

ABSTRACT Bordetella bronchiseptica (B. bronchiseptica) is an etiologic agent of respiratory diseases in animals and humans. Despite widespread use veterinary B. vaccines, there limited information on their composition, relative efficacy, the immune responses they elicit. Furthermore, human vaccines are not available. We leveraged dual antigenic adjuvant functions BcfA to develop acellular absence additional adjuvant. Balb/c mice immunized with alone or a trivalent vaccine containing antigens...

Vaccines are a 20th century medical marvel. They have dramatically reduced the morbidity and mortality caused by infectious diseases contributed to striking increase in life expectancy around globe. Nonetheless, determining vaccine efficacy remains challenge. Emerging evidence suggests that current acellular (aPV) for Bordetella pertussis (B. pertussis) induces suboptimal immunity. Therefore, major challenge is designing next-generation protective immunity without adverse side effects of...

Abstract Bordetella pertussis is the causative agent of whooping cough, a resurging vaccine-preventable disease. This highly contagious disease most severe in infants and young children. Current alum-adjuvanted acellular vaccines (aPV) prevent but do not nasal carriage subsequent person-to-person pathogen transmission. The Th1/2 skewed immune response induced by aPV one potential explanation for this failure. We are testing hypothesis that modification current addition an adjuvant, BcfA,...