A5070820810- Reproductive tract infections research
- Reproductive System and Pregnancy
- Reproductive Physiology in Livestock
- Cervical Cancer and HPV Research
- Genetic Associations and Epidemiology
- Tuberculosis Research and Epidemiology
- Genomic variations and chromosomal abnormalities
- Gene expression and cancer classification
- Genomics and Rare Diseases
- Bioinformatics and Genomic Networks
- Genital Health and Disease
- Genetic and phenotypic traits in livestock
- Enzyme Structure and Function
- Mycobacterium research and diagnosis
- Cancer-related molecular mechanisms research
- Genetic Mapping and Diversity in Plants and Animals
- Hepatitis B Virus Studies
- Adolescent Sexual and Reproductive Health
- Endometrial and Cervical Cancer Treatments
- Ovarian cancer diagnosis and treatment
- MicroRNA in disease regulation
- Preterm Birth and Chorioamnionitis
- Prenatal Screening and Diagnostics
- Biotin and Related Studies
- Congenital heart defects research
University of North Carolina at Chapel Hill
2015-2025
Sun Yat-sen University
2021-2024
Sun Yat-sen University Cancer Center
2021-2024
State Key Laboratory of Oncology in South China
2022-2024
Third Affiliated Hospital of Sun Yat-sen University
2024
AbbVie (United States)
2023
Xi'an Jiaotong University
2023
Qinghai University
2023
Weifang Medical University
2021-2022
Chongqing Emergency Medical Center
2022
Pdcd4 is a novel transformation suppressor that inhibits tumor promoter-induced neoplastic and the activation of AP-1-dependent transcription required for transformation. A yeast two-hybrid analysis revealed associates with eukaryotic translation initiation factors eIF4AI eIF4AII. Immunofluorescent confocal microscopy showed colocalizes eIF4A in cytoplasm. an ATP-dependent RNA helicase needed to unwind 5' mRNA secondary structure. Recombinant specifically inhibited activity eIF4F. In vivo...
Abstract Motivation: Microarray technology has been widely applied in biological and clinical studies for simultaneous monitoring of gene expression thousands genes. Gene clustering analysis is found useful discovering groups correlated genes potentially co-regulated or associated to the disease conditions under investigation. Many methods including hierarchical clustering, K-means, PAM, SOM, mixture model-based tight have used literature. Yet no comprehensive comparative study performed...
Chlamydia trachomatis genital tract infection is a major cause of female reproductive morbidity. Risk factors for ascending are unknown, and the role antibody in protection not well established.We recruited 225 women from urban outpatient clinics followed them median 12 months. We performed cross-sectional analysis serum anti-chlamydial immunoglobulin G (IgG), behavioral factors, microbiological associated with endometrial at enrollment, longitudinal incident infection.Oral contraceptives...
Natural infection induces partial immunity to Chlamydia trachomatis Identification of chlamydial antigens that induce interferon γ (IFN-) secretion by T cells from immune women could advance vaccine development. IFN-γ production CD4+ and CD8+ peripheral blood 58 high-risk was measured after coculture with antigen-presenting preincubated recombinant Escherichia coli expressing a panel 275 antigens. Quantile median regression analysis used compare frequencies responses in only cervical those...
An immunosuppressive tumor microenvironment in ovarian cancer facilitates progression and resistance to immunotherapy. The function of MYB Proto-Oncogene Like 2 (MYBL2) the remains largely unexplored.A syngeneic intraovarian mouse model, flow cytometry analysis, immunohistochemistry were used explore biological MYBL2 immune escape. Molecular biochemical strategies-namely RNA-sequencing, western blotting, quantitative reverse transcription-polymerase chain reaction (qRT-PCR), enzyme-linked...
Abstract Platinum resistance represents a major barrier to the survival of patients with ovarian cancer (OC). Cdc2‐like kinase 2 (CLK2) is protein associated oncogenic phenotype and development in some solid tumors. However, exact role underlying mechanism CLK2 progression OC currently unknown. Using microarray gene expression profiling immunostaining on tissues, we found that was upregulated tissues short platinum‐free interval patients. Functional assays showed protected cells from...
Cyclin-dependent kinase 5 (Cdk5) is a ubiquitously expressed serine/threonine kinase. However, requirement for Cdk5 has been demonstrated only in postmitotic neurons where there abundant expression of its activating partners p35 and/or p39. Although hyperactivation the Cdk5–p35 complex found variety inflammatory neurodegenerative disorders, potential contribution nonneuronal activity not explored this context. We describe previously unknown function T cells that required induction...
<i>Aim:</i> Our goal wasto produce a field synopsis of genetic associations with preterm birth and to set up publicly available online database summarizing the data. <i>Methods:</i> We performed systematic review meta-analyses identify birth. have association data on called PTBGene (http://ric.einstein.yu.edu/ptbgene/index.html) report structured thereof as December 1, 2008. <i>Results:</i> Data 189 polymorphisms in 84 genes been included 36 performed....
Chlamydia trachomatis can cause reproductive morbidities after ascending to the upper genital tract of women, and repeated infection lead worse disease. Data related protective immune responses at cervical mucosa that could limit chlamydial cervix and/or prevent reinfection inform vaccine approaches biomarkers risk.We measured 48 cytokines in secretions from women having alone (n = 92) or both endometrial 68). Univariable regression identified associated with differential odds risk,...
Problem Chlamydia infections in women can ascend to the upper genital tract, and repeated are common, placing at risk for sequelae. The protective role of anti‐chlamydia antibodies surface exposed antigens ascending incident infection is unclear. Method study A whole‐bacterial ELISA was used quantify chlamydia‐specific IgG IgA serum cervical secretions 151 high‐risk followed longitudinally. Correlations were determined between antibody burden, causal mediation analysis investigated effect on...
Chlamydia trachomatis (CT) is a globally prevalent sexually transmitted infection that can result in pelvic inflammatory disease, ectopic pregnancy, and infertility women. Currently, there no prophylactic vaccine. This study examined T-cell immunity cohort of women recently infected with CT. Participants were screened against peptides spanning 33 894 possible CT proteins, either ex vivo or using short-term cell lines. CT-specific T cells characterized by interferon (IFN) γ enzyme-linked...
<ns3:p>Background Our understanding of the human immune system’s response to viral respiratory tract infections (VRTIs) and vaccines, including molecular mechanisms correlates protection, remains incomplete. Extensive transcriptomic data from inoculation vaccination studies have been deposited in publicly available databases. However, these are often separate difficult locate. Methods To bridge this research gap, we systematically searched reviewed datasets NCBI Gene Expression Omnibus...
Abstract Raman microscopy was used to follow conformational changes in single protein crystals. Crystals of native insulin and the 5S 12S subunits enzyme transcarboxylase showed a mixture marker bands signifying α‐helix, β‐sheet nonordered secondary structure. However, by reducing S–S bonds crystal, or lowering pH for soaking substrates into structure each crystal became predominantly β‐sheet. The β‐form crystals could be dissolved only with difficulty yielded high–molecular weight...
Tens of thousands reproducibly identified GWAS (Genome-Wide Association Studies) variants, with the vast majority falling in non-coding regions resulting no eventual protein products, call urgently for mechanistic interpretations. Although numerous methods exist, there are few, if any methods, simultaneously testing mediation effects multiple correlated SNPs via some mediator (e.g. expression a gene neighborhood) on phenotypic outcome. We propose multi-SNP intersection-union test (SMUT) to...
Temporal lobe epilepsy (TLE) often occurs in childhood and is the most common type of epilepsy. Studies have confirmed that long non-coding RNAs (lncRNAs) can affect progression neurological diseases. This study explored expression level lncRNA TUG1 TLE children its clinical significance investigated role hippocampal neurons. 86 healthy individuals 88 were recruited. The expressions miR-199a-3p serum detected by qRT-PCR. Hippocampal neurons treated with non-Mg2+ to establish cell model. MTT...
Background: The systemic immune-inflammation index (SII) has been identified as a predictor of chemotherapy efficacy for variety cancers, and we aimed to determine its ability predict the response long-term prognosis patients with cervical squamous cell carcinoma (CSCC) who have underwent platinum-based neoadjuvant (NACT). Methods: date from 210 (133 in training cohort 77 validation cohort) CSCC received NACT were analyzed retrospectively. association between SII pathological complete (pCR)...
Background Tislelizumab is an anti-programmed cell death 1 (PD-1) monoclonal antibody engineered to minimize binding Fcγ receptors. It has been used treat several solid tumors. However, its efficacy and toxicity, the predictive prognostic value of baseline hematological parameters in patients with recurrent or metastatic cervical cancer (R/M CC) receiving tislelizumab remain unclear. Methods We reviewed 115 treated for R/M CC from March 2020 June 2022 our institute. The antitumor activity...
Chlamydia trachomatis (CT) is the most common bacterial sexually transmitted infection globally. Understanding natural immunity to CT will inform vaccine design. This study aimed profile immune cells and associated functional features in CT-infected women, determine profiles with reduced risk of ascended endometrial reinfection. PBMCs from CT-exposed women were profiled by mass cytometry random forest models identified key that distinguish outcomes. CT+ participants exhibited higher...
Abstract Accurate deconvolution of cell types from bulk gene expression is crucial for understanding cellular compositions and uncovering cell-type specific differential physiological states diseased tissues. Existing methods have limitations, such as requiring complete signatures or neglecting partial biological information. Moreover, these often overlook varying messenger RNA amounts, leading to biased proportion estimates. Additionally, they do not effectively utilize valuable reference...
About 40–60% of patients with late-onset Alzheimer’s disease (AD) develop psychosis, which represents a distinct phenotype more severe cognitive and functional deficits. The estimated heritability AD+P is ~61%, makes it good target for genetic mapping. We performed genome-wide copy-number variation (CNV) study on 496 AD cases psychosis (AD+P), 639 subjects intermediate (AD P) 156 without (AD−P) who were recruited at the University Pittsburgh Alzheimer's Disease Research Center using over 1...
N6-methyladenosine (m6A) is a highly abundant RNA modification in eukaryotic cells. Methyltransferase-like 3 (METTL3), major protein the m6A methyltransferase complex, plays important roles many malignancies, but its role cervical cancer metastasis remains uncertain. Here, we found that METTL3 was significantly upregulated tissue, and upregulation associated with poor prognosis patients. Knockdown of reduced cell migration invasion. Conversely, overexpression markedly promoted vitro vivo....