A5007488660- Immune Cell Function and Interaction
- HIV Research and Treatment
- T-cell and B-cell Immunology
- HIV/AIDS drug development and treatment
- NF-κB Signaling Pathways
- Immune Response and Inflammation
- HIV-related health complications and treatments
- HIV/AIDS Research and Interventions
- interferon and immune responses
- Complement system in diseases
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Renal Diseases and Glomerulopathies
- Virology and Viral Diseases
- Reproductive System and Pregnancy
- vaccines and immunoinformatics approaches
- Plant and animal studies
- Reproductive tract infections research
- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- MicroRNA in disease regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Hepatitis C virus research
- Plant Micronutrient Interactions and Effects
- Enzyme Production and Characterization
Garvan Institute of Medical Research
2015-2024
University of North Carolina at Chapel Hill
2017-2024
University of Central Florida
2024
University of Central Arkansas
2024
Conway School of Landscape Design
2024
Jacksonville State University
2024
UNSW Sydney
2019-2022
St Vincent's Clinic
2019-2022
University of North Carolina Health Care
2020
Indiana University School of Medicine
2017
Abstract Gene-targeted C1q-deficient mice have been shown to develop a syndrome reminiscent of human systemic lupus erythematosus with antinuclear Abs and proliferative glomerulonephritis. Initial phenotypic analysis conducted in (129 × C57BL/6) hybrid showed that background genes were significant factor for the full expression autoimmune disease. To assess contribution phenotype, disrupted C1qa gene was backcrossed seven generations onto C57BL/6 MRL/Mp+/+ strains. These intercrossed...
Systemic lupus erythematosus (SLE) is a multisystem autoimmune disorder in which complex genetic factors play an important role. Several strains of gene-targeted mice have been reported to develop SLE, implicating the null genes causation disease. However, hybrid between 129 and C57BL/6 mice, widely used generation spontaneous autoimmunity. Furthermore, background markedly influences phenotype SLE mice. This suggests role expression autoimmunity as-yet-uncharacterised originating from these...
Abstract T follicular regulatory (Tfr) cells control the magnitude and specificity of germinal centre reaction, but how regulation is contained to ensure generation high-affinity antibody unknown. Here we show that this balance maintained by reciprocal influence interleukin (IL)-2 IL-21. The number IL-2-dependent FoxP3 + increased in peripheral blood human patients with loss-of-function mutations IL-21 receptor (IL-21R). In mice, IL-21:IL-21R interactions phenotype cells, reducing expression...
Inoculation of embryonated eggs with toxoplasma resulted in a generalized parasitic disease this host fatal termination. The agent could be maintained by serial passage embryo brain. Chorioallantoic membranes from infected contained stable, specific soluble antigen which fixed complement immune animal serum. Human sera both proven and suspected cases toxoplasmosis were known to contain neutralizing antibody against the parasite also chorioallantoic membrane antigen.
Membranoproliferative glomerulonephritis (MPGN) type II (dense deposit disease) is an inflammatory renal disease characterized by electron-dense deposits and complement C3 on the glomerular basement membrane. There no effective therapy. We investigated role of C5 activation in a model MPGN that develops spontaneously factor H-deficient mice (Cfh(-/-)). At 12 months there was significant reduction mortality, cellularity, neutrophil numbers, serum creatinine levels Cfh(-/-) deficient C5....
Micro-RNAs (miRNAs) are frequently dysregulated in a range of human malignancies, many have been shown to act either as tumour supressors or oncogenes and several implicated breast cancer. However, cancer is diverse disease little known about the relationships between miRNA expression, clinical outcome subtype. We used locked nucleic acid probe situ hybridization (LNA-ISH) visualize, tissue micro-arrays (TMAs) 2919 formalin-fixed paraffin-embedded (FFPE) archival tumours, expression two key...
In order to inform the rational design of HIV-1 preventive and cure interventions it is critical understand events occurring during acute infection (AHI). Using viral deep sequencing on six participants from early capture RV217 cohort, we have studied evolution in plasma collected twice weekly first weeks following advent viremia. The analysis infections established by multiple transmitted/founder (T/F) viruses revealed novel profiles that included: a) low-level persistence minor T/F...
The selection of affinity-matured Ab-producing B cells is supported by interactions with T follicular helper (Tfh) cells. In addition to cell surface-expressed molecules, cytokines produced Tfh cells, such as IL-21 and IL-4, provide signals. this study, we analyze how the fitness Th can influence Ab responses. To do this, used a model in which IL-21R-sufficient (wild-type [WT]) -deficient (Il21r(-/-)) Ag-specific were help immunodeficient Il21r(-/-) following T-dependent immunization. that...
Approaches to deplete persistent HIV infection are needed. We investigated the combined impact of latency reversing agent vorinostat (VOR) and AGS-004, an autologous dendritic cell immunotherapeutic, on reservoir. HIV+, stably treated participants in whom resting CD4+ T cell-associated RNA (rca-RNA) increased after VOR exposure ex vivo received 4 doses AGS-004 every 3 weeks, followed by 72 hours for 30 days, then cycle repeated. Change VOR-responsive host gene expression, HIV-specific...
Freezing (−75 C) and storage (−20 of a cell suspension Salmonella gallinarum resulted in heterogeneous population dead, metabolically injured, unharmed cells. Injured cells constituted as much 40% those surviving freezing for 1 day. Replica plating frozen thawed indicated metabolic injury was repairable not stable mutation. Penicillin used to increase the ratio injured uninjured from suspension. Pathogenicity evaluated by observing per cent mortality after injecting or into separate sets...
Significance The use of specific IL-2/anti–IL-2 complexes (IL-2 cplxs) has shown great potency in the prevention allograft rejection mice. While long-term tolerance could be achieved toward islet allografts, clinically relevant skin graft models have remained elusive so far. Using a new protocol, this study documents that IL-2 cplxs can greatly prolong survival allografts and prevent sensitization recipient. data provide support for importance regulatory mechanisms organ-specific approaches...
Chlamydia trachomatis (CT) is a globally prevalent sexually transmitted infection that can result in pelvic inflammatory disease, ectopic pregnancy, and infertility women. Currently, there no prophylactic vaccine. This study examined T-cell immunity cohort of women recently infected with CT. Participants were screened against peptides spanning 33 894 possible CT proteins, either ex vivo or using short-term cell lines. CT-specific T cells characterized by interferon (IFN) γ enzyme-linked...
Formalin-fixed paraffin-embedded (FFPE) samples are valuable but underutilized in single-cell omics research due to their low RNA quality. In this study, leveraging a recent advance genomic technology, we introduce snPATHO-seq, versatile method derive high-quality single-nucleus transcriptomic data from FFPE samples. We benchmarked the performance of snPATHO-seq workflow against existing 10x 3' and Flex assays designed for frozen or fresh highlighted consistency snRNA-seq produced by all...
HIV-1-specific CD8+ T cells are an important component of HIV-1 curative strategies. Viral variants in the reservoir may limit capacity to detect and clear virus-infected cells. We investigated patterns cell escape replication-competent 25 persons living with (PLWH) durably suppressed on antiretroviral therapy (ART). identified all reactive epitopes proteome for each participant sequenced outgrowth viruses from resting CD4+ All non-synonymous mutations were tested their effect size response,...
NF-κB activation unites metabolic and inflammatory responses in many diseases yet less is known about the role that plays normal metabolism. In this study we investigated how RELA impacts beta cell transcriptional landscape provides network control over glucoregulation.We generated novel mouse lines harbouring cell-specific deletion of either Rela gene, encoding canonical transcription factor p65 (βp65KO mice), or Ikbkg essential modulator NEMO (βNEMOKO as well βA20Tg mice carry forced...
The role of the complement system in host defense against Salmonella infection is poorly defined. Bacterial cell wall O-antigen polysaccharide can activate alternative pathway vitro. No studies, however, have elucidated classical immunity to spp. vivo. C1q-deficient mice (C1qa(-/-)) on a 129/Sv genetic background and strain-matched controls were infected intraperitoneally intravenously with enterica serovar Typhimurium monitored over 14-day period. After inoculation by either route,...
Abstract IL-17–producing γδ T (γδT-17) cells have proved to be an important early source of IL-17 in many inflammatory settings and are emerging as participant protumor immune responses. Considering that their peripheral activation depends largely on innate signals rather than TCR ligation, it is understand what mechanisms exist curb unwanted activation. Expression the high-affinity IL-2R γδT-17 prompted us investigate a role for this cytokine. We found enriched, not depleted, IL-2–deficient...
ABSTRACT. CD59 is a complement regulatory protein that inhibits the terminal part of system, membrane attack complex (MAC), mediator renal injury. Mice deficient in <i>Cd59a</i> gene (<i>mCd59a</i>−/−) were used to investigate role experimentally induced accelerated nephrotoxic nephritis, model immune complex–mediated glomerulonephritis. After nephritis was by administration sheep globulin, <i>mCd59a</i>−/− mice and strain-matched controls on two genetic backgrounds, 129/Sv × C57BL/6 129/Sv,...
The archetypal T cell-dependent antigen is sheep red blood cells (SRBCs), which have defined much of what we know about humoral immunity. Early studies using solubilized or sonicated SRBCs argued that the intact structure was important for optimal antibody responses. However, reason requirement response to polyvalent protein remained unknown. Here, report immune driven by cytosolic recognition SRBC RNA through RIG-I-like receptor (RLR)-mitochondrial anti-viral signaling adaptor (MAVS)...