A5102766405- HIV Research and Treatment
- Immune Cell Function and Interaction
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Acute Myeloid Leukemia Research
- HIV-related health complications and treatments
- T-cell and B-cell Immunology
- Hematopoietic Stem Cell Transplantation
- Immunotherapy and Immune Responses
- Stress Responses and Cortisol
- RNA modifications and cancer
- Cytomegalovirus and herpesvirus research
- Hormonal Regulation and Hypertension
- Protein Tyrosine Phosphatases
- vaccines and immunoinformatics approaches
- Chronic Lymphocytic Leukemia Research
- Adipose Tissue and Metabolism
- Cancer Immunotherapy and Biomarkers
- Bioactive natural compounds
- Pregnancy and preeclampsia studies
- Hepatitis C virus research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Adolescent Sexual and Reproductive Health
- Reproductive System and Pregnancy
University of North Carolina at Chapel Hill
2015-2024
Nanjing Maternity and Child Health Care Hospital
2013-2022
Nanjing Medical University
2006-2022
Creative Commons
2020
Jilin University
2020
Johns Hopkins University Center for AIDS Research
2019
Nagoya University
2006-2018
University of North Carolina Health Care
2017
Kettering University
2009-2011
Obihiro National Hospital
2007
Blockade of inhibitory signals mediated by cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) has been shown to enhance cell responses and induce durable clinical in patients with metastatic melanoma. The functional impact anti-CTLA-4 therapy on human immune is still unclear. To explore this, we analyzed immune-related adverse events melanoma treated ipilimumab, a fully monoclonal antibody. Fifteen were selected the basis availability suitable specimens for immunologic monitoring, eight...
Ipilimumab, a monoclonal antibody against cytotoxic T lymphocyte antigen 4 (CTLA-4), has been shown to improve survival in patients with advanced metastatic melanoma. It also enhances immunity NY-ESO-1, cancer/testis expressed subset of To characterize the association between immune response and clinical outcome, we first analyzed NY-ESO-1 serum by ELISA 144 ipilimumab-treated melanoma found 22 140 (16%) seropositive at baseline 31 (22%) following treatment. These NY-ESO-1–seropositive had...
The histone deacetylase (HDAC) inhibitor vorinostat (VOR) can increase HIV RNA expression in vivo within resting CD4+ T cells of aviremic HIV+ individuals. However, while studies VOR or other HDAC inhibitors have reported reversal latency, none has demonstrated clearance latent infection. We sought to identify the optimal dosing for effective serial latency.In a study 16 HIV-infected, individuals, we measured cell-associated ex and following single exposure VOR, then after pair doses...
Berberine has drawn extensive attention toward their wide range of biochemical and pharmacological effects, including antineoplastic effect in recent years, but the precise mechanisms remain unclear. Treatment human breast cancer cells (MCF-7 MDA-MB-231 cells) with berberine induced inhibition cell viability concentration- time-dependent manner irrespective estrogen receptor (ER) expression. Hoechst33342 staining confirmed apoptosis manner. Because induction is considered to be a crucial...
Despite the extraordinary success of HIV-1 antiretroviral therapy in prolonging life, infected individuals face lifelong because a reservoir latently-infected cells that harbor replication competent virus. Recently, compounds have been identified can reverse latency vivo. These latency- reversing agents (LRAs) could make vulnerable to clearance by immune cells, including cytolytic CD8+ T cells. We investigated effects two leading LRA classes on cell phenotype and function: histone...
Acquired aplastic anemia is an immune-mediated disease characterized by severe defects in stem cell number resulting hypocellular marrow and peripheral blood cytopenias. Minor paroxysmal nocturnal hemoglobinuria populations a short telomere length were identified as predictive biomarkers of immunosuppressive therapy responsiveness anemia. We enrolled 113 patients (63 boys 50 girls) this study to evaluate their response therapy. The detected flow cytometry. Forty-seven (42%) carried minor...
Mutations in NF1, PTPN11, NRAS, KRAS and CBL have been reported to play a pathogenetic role juvenile myelomonocytic leukaemia (JMML), rare myelodyplastic/myeloproliferative neoplasm occurring children. Recently, mutations ASXL1 were identified chronic other myeloid malignancies. We sequenced exon 12 of ASLX1 49 JMML patients, found 2 novel heterozygous (nonsense frameshift) mutations, one as sole lesion, the was conjunction with PTPN11 mutation. cooperates KDM1A transcriptional repression...
Predicting the response to immunosuppressive therapy could provide useful information help clinician define treatment strategies for patients with aplastic anemia. In our current study, we evaluated relationship between telomere length of lymphocytes at diagnosis and in 64 children anemia, using flow fluorescence situ hybridization. Median age was ten years (range 1.5-16.2 years). Severity disease classified as very severe 23, 21, moderate 20 patients. All were enrolled multicenter studies...
Chlamydia trachomatis (CT) is a globally prevalent sexually transmitted infection that can result in pelvic inflammatory disease, ectopic pregnancy, and infertility women. Currently, there no prophylactic vaccine. This study examined T-cell immunity cohort of women recently infected with CT. Participants were screened against peptides spanning 33 894 possible CT proteins, either ex vivo or using short-term cell lines. CT-specific T cells characterized by interferon (IFN) γ enzyme-linked...
Urocortin (UCN) functions via corticotrophin-releasing factor receptors (CRFRs), CRFR1 & 2. CRFR2 is reported to be a tonic suppressor of vascularization, implying its role in tumor angiogenesis. Here, it was found that UCN inhibited the growth hepatocellular carcinoma (HCC) and reduced microvessel density nude mice. Hepatoma cells didn't express CRFRs whereas vessels expressed CRFRs, mainly CRFR2. In vitro three-dimensional culture assay showed angiogenesis, this effect abolished by...
We assessed the clinical utility of next-generation sequencing (NGS)-based monitoring minimal residual disease (MRD) in a uniformly treated cohort 79 patients with paediatric B-cell acute lymphoblastic leukaemia. Bone marrow samples were collected at time diagnosis, days 33 and 80, pre- (4-5 months) post- (24 maintenance therapy points, relapse. identified leukaemia-specific CDR3 sequences 72 (91%) detected MRD 59 232 samples. Although was 28 55 (51%) on day 33, frequencies detection...
HIV-1-specific CD8+ T cells are an important component of HIV-1 curative strategies. Viral variants in the reservoir may limit capacity to detect and clear virus-infected cells. We investigated patterns cell escape replication-competent 25 persons living with (PLWH) durably suppressed on antiretroviral therapy (ART). identified all reactive epitopes proteome for each participant sequenced outgrowth viruses from resting CD4+ All non-synonymous mutations were tested their effect size response,...
Autophagy, identified as type II programmed cell death, has already been known to be involved in the pathophysiology of preeclampsia (PE), which is a gestational disease with high morbidity. The present study aims investigate functional role let‐7i, miRNA, trophoblastic autophagy. Placental tissue used this was collected from patients severe (SPE) or normal pregnant women. A decreased level let‐7i found placenta SPE. In addition, autophagic vacuoles were observed SPE and expression...