A5055817938- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Renal Transplantation Outcomes and Treatments
- Hematopoietic Stem Cell Transplantation
- Immunotherapy and Immune Responses
- Allergic Rhinitis and Sensitization
- Transplantation: Methods and Outcomes
- Mesenchymal stem cell research
- Organ Transplantation Techniques and Outcomes
- Liver physiology and pathology
- Organ and Tissue Transplantation Research
- CAR-T cell therapy research
- Reproductive System and Pregnancy
- Cancer Genomics and Diagnostics
- Asthma and respiratory diseases
- Colorectal Cancer Treatments and Studies
- Food Allergy and Anaphylaxis Research
- Cytomegalovirus and herpesvirus research
- Hepatocellular Carcinoma Treatment and Prognosis
- Peptidase Inhibition and Analysis
- Mast cells and histamine
- Pluripotent Stem Cells Research
- Sodium Intake and Health
- Virus-based gene therapy research
- Complement system in diseases
Medical University of Vienna
2016-2025
Garvan Institute of Medical Research
2019-2021
St Vincent's Clinic
2019
UNSW Sydney
2019
Vienna General Hospital
2009-2012
Foundation for Human Potential
2009
Fachhochschule Wiener Neustadt
2009
Secondary, so-called costimulatory, signals are critically required for the process of T cell activation. Since landmark studies defined that cells receiving a receptor signal without costimulatory signal, tolerized in vitro, investigation costimulation has attracted intense interest. Early demonstrated interrupting allows attenuation alloresponse, which is particularly difficult to modulate due clone size alloreactive cells. The understanding since evolved substantially and now encompasses...
Costimulatory blockade of CD28-B7 interaction with CTLA4Ig is a well-established strategy to induce transplantation tolerance. Although previous in vitro studies suggest that upregulates expression the immunoregulatory enzyme IDO dendritic cells, relationship and vivo organ remains unclear. In this study, we studied whether concerted immunomodulation by depends on IDO. C57BL/6 recipients receiving fully MHC-mismatched BALB/c heart graft treated + donor-specific transfusion showed indefinite...
In this randomized placebo-controlled crossover study, we evaluated whether previously reported cardiovascular benefits of plant-derived inorganic nitrate via NO translate into improved vascular function and blood pressure-lowering in middle-aged older men women with treated hypertension.
Vascular oxidative stress and low-grade inflammation are important in the pathology of cardiovascular disorders, including hypertension. Cell culture animal studies suggest that inorganic dietary nitrate may attenuate through nitric oxide (NO), there is a need to investigate whether this translates humans. In randomised, placebo-controlled crossover study, by measuring combination multiple blood biomarkers, we evaluated previously reported benefits translate reduced an improved status 15 men...
Although γδ T cells are known to participate in immune dysregulation solid tumors, their relevance human microsatellite-stable (MSS) colorectal cancer (CRC) is still undefined. Here, using integrated gene expression analysis and cell receptor sequencing, we characterized MSS CRC, with a focus on Vδ1 + cells. We identified Vδ1+ shared motifs the third complementarity-determining region of δ-chain, reflective antigen recognition. Changes protein levels suggested dysfunctional effector state...
BackgroundThe mixed chimerism approach induces donor-specific tolerance in both pre-clinical models and clinical pilot trials. However, chronic rejection of heart allografts acute skin were observed some chimeric animals despite persistent hematopoietic toward donor antigens vitro. We tested whether additional cell therapy with regulatory T cells (Tregs) is able to induce full immunologic prevent rejection.MethodsWe recently developed a murine "Treg bone marrow (BM) transplantation (BMT)...
Significance The use of specific IL-2/anti–IL-2 complexes (IL-2 cplxs) has shown great potency in the prevention allograft rejection mice. While long-term tolerance could be achieved toward islet allografts, clinically relevant skin graft models have remained elusive so far. Using a new protocol, this study documents that IL-2 cplxs can greatly prolong survival allografts and prevent sensitization recipient. data provide support for importance regulatory mechanisms organ-specific approaches...
Abstract Allergy represents a hypersensitivity disease that affects >25% of the population in industrialized countries. The underlying type I allergic immune reaction occurs predisposed atopic individuals response to otherwise harmless Ags (i.e., allergens) and is characterized by production allergen-specific IgE, an T cell response, release biologically active mediators such as histamine from mast cells basophils. Regimens permanently tolerizing still need be developed. We therefore...
B7.1/2-targeted costimulation blockade (CTLA4 immunoglobulin [CTLA4-Ig]) is available for immunosuppression after kidney transplantation, but its potentially detrimental impact on regulatory T cells (Tregs) of concern. We investigated the effects CTLA4-Ig monotherapy in a fully mismatched heart transplant model (BALB/c onto C57BL/6). was injected chronically (on days 0, 4, 14, and 28 every 4 weeks thereafter) dosing regimens paralleling clinical use, shown per mouse: low dose (LD), 0.25 mg...
Organ transplantation is a surgery in which doctors replace person’s sick or damaged organ with healthy from another person. The new can come living person who wants to help, someone whose brain died and thus does not need their organs anymore. main problem transplants rejection, happens when the immune system treats like foreign invader such as an infection tries remove it. To prevent patients received transplant take many pills for rest of lives. These pills, called immunosuppressants,...
The mixed chimerism approach achieves donor-specific tolerance in organ transplantation, but clinical use is inhibited by the toxicities of current bone marrow (BM) transplantation (BMT) protocols. Blocking CD40:CD154 pathway with anti-CD154 monoclonal antibodies (mAbs) exceptionally potent inducing chimerism, these mAbs are clinically not available. Defining roles donor and recipient CD40 a murine allogeneic BMT model, we show that CD4 or CD8 activation through an intact direct T cell...
Background. Mixed chimerism is an effective strategy for the induction of transplantation tolerance but toxicity recipient conditioning makes current bone marrow (BM) (BMT) protocols unsuitable widespread clinical application. Therapies promoting BM engraftment under minimal would facilitate translation this concept to clinic. Recently, we have shown that regulatory T cell (Treg) therapy has potent engraftment-enhancing effects in irradiation-free noncytotoxic BMT protocol, only if it...
Immunosuppressive cytotoxic T lymphocyte associated antigen-4 immunoglobulin fusion proteins (CTLA4-Ig) block the CD28:CD80/86 costimulatory pathway. On a cellular level, CTLA4-Ig is understood to dampen cell responses. As mechanism, has been reported affect dendritic (DC) function via inducing immunosuppressive indoleamine 2,3 dioxygenase (IDO) pathway and promoting DC regulatory phenotype. We here probed mechanisms of immunoregulation in an allogeneic setting using C57BL/6 splenic or bone...
Since the discovery of regulatory T cells (Tregs) as crucial regulators immune tolerance against self-antigens, these have become a promising tool for induction donor-specific in transplantation medicine. The therapeutic potential increasing vivoTreg numbers favorable Treg to Teff cell ratio has already been demonstrated several sophisticated pre-clinical models and clinical pilot trials. In addition improving quantity, enhancing function utilizing engineering techniques led encouraging...
Donor-specific antibodies of the IgG isotype are measured routinely for diagnostic purposes in renal transplant recipients and associated with antibody-mediated rejection long-term graft loss.