A5049773661- Pancreatic and Hepatic Oncology Research
- Oral microbiology and periodontitis research
- Gut microbiota and health
- Immune Cell Function and Interaction
- Cancer Research and Treatments
- Immune cells in cancer
- Salivary Gland Disorders and Functions
- CAR-T cell therapy research
- Influenza Virus Research Studies
- Oral Health Pathology and Treatment
- vaccines and immunoinformatics approaches
- Cancer Immunotherapy and Biomarkers
- Bone health and treatments
- Pancreatitis Pathology and Treatment
- Phagocytosis and Immune Regulation
- Bone Tissue Engineering Materials
- Oral and Maxillofacial Pathology
- COVID-19 Clinical Research Studies
- Dental Implant Techniques and Outcomes
- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- Smoking Behavior and Cessation
- Advanced Proteomics Techniques and Applications
- Dental materials and restorations
- Long-Term Effects of COVID-19
New York University
2014-2024
We found that the cancerous pancreas harbors a markedly more abundant microbiome compared with normal in both mice and humans, select bacteria are differentially increased tumorous gut. Ablation of protects against preinvasive invasive pancreatic ductal adenocarcinoma (PDA), whereas transfer from PDA-bearing hosts, but not controls, reverses tumor protection. Bacterial ablation was associated immunogenic reprogramming PDA microenvironment, including reduction myeloid-derived suppressor cells...
Abstract Background Bacterial infections have been linked to malignancies due their ability induce chronic inflammation. We investigated the association of oral bacteria in squamous cell carcinoma (OSCC/tumor) tissues and compared with adjacent non-tumor mucosa sampled 5 cm distant from same patient ( n = 10 ). By using culture-independent 16S rRNA approaches, denaturing gradient gel electrophoresis (DGGE) cloning sequencing, we assessed total bacterial diversity these clinical samples....
In the oral cavity, chronic inflammation has been observed at various stages of squamous cell carcinomas (OSCC). Such could result from persistent mucosal or epithelial colonization by microorganisms. There is increasing evidence involvement bacteria in inflammation, warranting further studies on association with progression OSCC. The objective this study was to evaluate diversity and relative abundance saliva subjects Using 454 parallel DNA sequencing, ∼58,000 PCR amplicons that span V4-V5...
Modulation of Toll-like receptor (TLR) signaling can have protective or protumorigenic effects on oncogenesis depending the cancer subtype and specific inflammatory elements within tumor milieu. We found that TLR9 is widely expressed early during course pancreatic transformation ligands are ubiquitous microenvironment. ligation markedly accelerates oncogenesis, whereas deletion protective. show activation has distinct epithelial, inflammatory, fibrogenic cellular subsets in carcinoma plays a...
The trend of e-cigarette use among teens is ever increasing. Here we show the dysbiotic oral microbial ecology in users influencing local host immune environment compared with non-smoker controls and cigarette smokers. Using 16S rRNA high-throughput sequencing, evaluated 119 human participants, 40 each three cohorts, found significantly altered beta-diversity (p = 0.006) when never smokers or tobacco abundance Porphyromonas Veillonella 0.008) was higher vapers. Interleukin (IL)-6 IL-1β were...
Blockade of the Piezo1 mechanoreceptor decreases myeloid cell suppression immune responses to cancer and polymicrobial sepsis.
Various surface modifications have been tried for enhancing osseointegration of the dental implants like mechanical and/or chemical treatments and deposition calcium phosphate coatings. The objective this research was to develop calcium-phosphate based thin coatings with antibacterial bioactive properties potential application in implants. Titanium (Ti) discs were immersed different calcifying solutions: CaP (positive control), F-CaP, Zn-CaP, FZn-CaP incubated 24 h. Negative control uncoated...
The gut microbiome shapes local and systemic immunity. liver is presumed to be a protected sterile site. As such, hepatic has not been examined. Here, we showed in mice humans that distinct from of the enriched Proteobacteria. It undergoes dynamic alterations with age influenced by environment host physiology. Fecal microbial transfer experiments revealed populated highly selective manner. Hepatic immunity dependent on microbiome, specifically bacteroidetes species. Targeting oral...
Electronic cigarettes (e-cigs) have become prevalent as an alternative to conventional cigarette smoking, particularly in youth. E-cig aerosols contain unique chemicals which alter the oral microbiome and promote dysbiosis ways we are just beginning investigate. We conducted a 6-month longitudinal study involving 84 subjects who were either e-cig users, smokers, or nonsmokers. Periodontal condition, cytokine levels, subgingival microbial community composition assessed, with periodontal,...
Oral Diseases (2012) 18, 602–612 Objective: Infection has been hypothesized as a contributing factor to bisphosphonate (BP)‐related osteonecrosis of the jaw (BRONJ). The objective this study was determine bacterial colonization jawbone and identify phylotypes associated with BRONJ. Materials methods: Culture‐independent 16S rRNA gene‐based molecular techniques were used compare total diversity in bone samples collected from 12 patients cancer (six, BRONJ history BP; six, controls without...
Oral Diseases (2011) 18 , 85–95 Objective: infection is considered to play a critical role in the pathogenesis of bisphosphonate‐related osteonecrosis jaw (BRONJ), and antibiotic therapy has become mainstay BRONJ therapy. This study was aimed investigate effect antibiotics on bacterial diversity tissues. Materials methods: The profile from soft tissues associated with lesion determined using 16S rRNA‐based denaturing gradient gel electrophoresis (DGGE) sequencing. Twenty subjects classified...
Bacterial biofilms have emerged as potential critical triggers in the pathogenesis of bisphosphonate (BP)-related osteonecrosis jaw (ONJ) or BRONJ. BRONJ lesions shown to be heavily colonized by oral bacteria, most these difficult cultivate and presents many clinical challenges. The purpose this study was characterize bacterial diversity determine host immune response. We examined tissue specimens from three cohorts (n=30); patients with periodontal disease without a history BP therapy...
There is currently no criterion to select appropriate bioinformatics tools and reference databases for analysis of 16S rRNA amplicon data in the human oral microbiome. Our study aims determine influence multiple on α-diversity measurements β-diversity comparisons analyzing We compared results taxonomical classification by Greengenes, Human Oral Microbiome Database (HOMD), National Center Biotechnology Information (NCBI) 16S, SILVA, Ribosomal Project (RDP) using Quantitative Insights Into...
Periodontal disease is a chronic, inflammatory bacterial dysbiosis that associated with both Alzheimer's (AD) and Down syndrome.A total of 48 elderly cognitively normal subjects were evaluated for differences in subgingival periodontal bacteria (assayed by 16S rRNA sequencing) between cerebrospinal fluid (CSF) biomarker groups amyloid neurofibrillary pathology. A dysbiotic index (DI) was defined at the genus level as abundance ratio known to healthy bacteria. Analysis variance/analysis...
Periodontal disease (PerioD) is a chronic inflammatory of dysbiotic etiology. Animal models and few human data showed relationship between oral bacteria gut dysbiosis. However, the effect periodontal inflammation subgingival dysbiosis on unknown. We hypothesized that its associated contribute to even in subjects free known disorders. evaluated compared elderly with Low High (assessed by Inflamed Surface Area (PISA)) for stool derived (assayed 16S rRNA sequencing). The associations...
The effect of electronic cigarette (e-cigarette) smoking, especially its long-term impact on oral health, is poorly understood. Here, we conducted a longitudinal clinical study with two visits, 6 months apart, to investigate the e-cigarette use bacterial community structure in saliva 101 periodontitis patients. Our data demonstrated that altered microbiome patients, enriching members Filifactor, Treponema, and Fusobacterium taxa. For patients at same periodontal disease stage, smokers shared...
A key to improving vaccine design and vaccination strategy is understand the mechanism behind variation of response with host factors. Glycosylation, a critical modulator immunity, has no clear role in determining responses. To gain insight into association between glycosylation vaccine-induced antibody levels, we profiled pre- postvaccination serum protein glycomes 160 Caucasian adults receiving FLUZONE influenza during 2019-2020 season using lectin microarray technology. We found that...
Singlet oxygen is a potent agent for the selective killing of wide range harmful cells; however, current delivery methods pose significant obstacles to its widespread use as treatment agent. Limitations include need photosensitizer proximity tissue because short (3.5 μs) lifetime singlet in contact with water; strong optical absorption photosensitizer, which limits penetration depth; and hypoxic environments that restrict concentration available oxygen. In this article, we describe novel...
Abstract The COVID-19 pandemic, triggered by severe acute respiratory syndrome coronavirus 2, has affected millions of people worldwide. Much research been dedicated to our understanding disease heterogeneity and severity, but less is known about recovery associated changes. To address this gap in knowledge, we quantified the proteome from serum samples 29 convalescents age-, race-, sex-matched healthy controls. Samples were acquired within first months pandemic. Many proteins pathways...
Abstract Background Evaluate the effect of low‐temperature plasma (LTP) on an anaerobic biofilm and biological response in vitro reconstituted gingival epithelium tissue. Methods Porphyromonas gingivalis W83 was cultured titanium discs tissues were submitted to similar treatment conditions. Treatments: LTP1—plasma for 1 minute, LTP3—plasma 3 CHX—0.2% chlorhexidine GAS—gas only (no plasma) NEGATIVE—no treatment. TRITON group included as a positive control tissue analysis. Counting viable...
Abstract Unconventional T-lymphocyte populations are emerging as important regulators of tumor immunity. Despite this, the role TCRαβ+CD4−CD8−NK1.1− innate αβ T cells (iαβT) in pancreatic ductal adenocarcinoma (PDA) has not been explored. We found that iαβTs represent ∼10% lymphocytes infiltrating PDA mice and humans. Intratumoral express a distinct T-cell receptor repertoire profoundly immunogenic phenotype compared with their peripheral counterparts conventional lymphocytes. comprised ∼75%...
Introduction: Tobacco use is one of the main causes periodontitis. E-cigarette are gaining in popularity, and studies needed to better understand impact e-cigarettes on oral health. Objective: To perform a longitudinal study evaluate adverse effects periodontal Methods: Naïve users, cigarette smokers, non-smokers were recruited using newspaper social media. Age, gender, ethnicity, recorded. Participants scheduled for two visits 6 months apart. At each visit, we collected data frequency...
Abstract We have developed a calcium phosphate glass (CPG) doped with Zn 2+ or F − combined and ions, which are naturally found in the human body play dual role bone formation antibacterial activity. Previously, we demonstrated that this family of CPGs has superior osteoconductive resorbable properties vivo . This study aimed to investigate property incorporating and/or used Streptococcus mutans as model organism because it is one major oral pathogens an early colonizer, been associated...