A5084662043- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Hepatitis C virus research
- Hepatitis B Virus Studies
- COVID-19 epidemiological studies
- Immunotherapy and Immune Responses
- Prion Diseases and Protein Misfolding
- Biosensors and Analytical Detection
- HIV Research and Treatment
- Travel-related health issues
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- Cancer Research and Treatments
- HIV/AIDS drug development and treatment
- Immune Cell Function and Interaction
- Respiratory viral infections research
- vaccines and immunoinformatics approaches
- Advanced Drug Delivery Systems
- Renal Transplantation Outcomes and Treatments
- Reproductive tract infections research
- Primate Behavior and Ecology
- Seaweed-derived Bioactive Compounds
- Amphibian and Reptile Biology
- T-cell and B-cell Immunology
Imperial College London
2020-2024
St Mary's Hospital
2021
Indira Gandhi Medical College
2018
Public Health England
2013-2017
MRC Prion Unit
2016
University College London
2016
Google (United States)
2015
National Institute for Biological Standards and Control
2008-2015
Accurate antibody tests are essential to monitor the SARS-CoV-2 pandemic. Lateral flow immunoassays (LFIAs) can deliver testing at scale. However, reported performance varies, and sensitivity analyses have generally been conducted on serum from hospitalised patients. For use in community testing, evaluation of finger-prick self-tests, non-hospitalised individuals, is required.Sensitivity analysis was 276 participants. All had tested positive for by reverse transcription PCR were ≥21 days...
Knowledge of the window SARS-CoV-2 infectiousness is crucial in developing policies to curb transmission. Mathematical modelling based on scarce empirical evidence and key assumptions has driven isolation testing policy, but real-world data are needed. We aimed characterise across full course infection a community setting.The Assessment Transmission Contagiousness COVID-19 Contacts (ATACCC) study was UK prospective, longitudinal, cohort contacts newly diagnosed, PCR-confirmed index cases....
ABSTRACT Affordable next-generation sequencing (NGS) technologies for hepatitis C virus (HCV) may potentially identify both viral genotype and resistance genetic motifs in the era of directly acting antiviral (DAA) therapies. This study compared ability high-throughput NGS methods to generate full-length, deep, HCV sequence data sets evaluated their utility diagnostics clinical assessment. using (i) unselected RNA (metagenomics), (ii) preenrichment by probe capture, (iii) preamplification...
Access to rapid diagnosis is key the control and management of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Laboratory RT-PCR testing current standard care but usually requires a centralised laboratory significant infrastructure. We describe our diagnostic accuracy assessment novel, point-of-care real time CovidNudge test, which no handling or sample pre-processing.
To evaluate the performance of new lateral flow immunoassays (LFIAs) suitable for use in a national coronavirus disease 2019 (covid-19) seroprevalence programme (real time assessment community transmission 2-React 2). Diagnostic accuracy study. Laboratory analyses were performed United Kingdom at Imperial College, London and university facilities London. Research clinics finger prick sampling run two affiliated NHS trusts. Sensitivity on sera stored from 320 previous participants React 2...
Abstract We report on single-molecule nanopore sensing combined with position-encoded DNA molecular probes, chemistry tuned to simultaneously identify various antigen proteins and multiple RNA gene fragments of SARS-CoV-2 high sensitivity selectivity. show that this strategy can directly detect spike (S) nucleocapsid (N) in unprocessed human saliva. Moreover, our approach enables the identification from patient samples using nasal/throat swabs, enabling critical mutations such as D614G,...
Abstract Background Mauritian cynomolgus macaques have greatly restricted genetic diversity in the MHC region compared to other non‐human primates; however, frequency of common haplotypes among captive‐bred populations has not been reported. Methods Microsatellite PCR was used determine haplotype frequencies captive at a UK breeding facility. Allele‐specific and reference strand conformational analysis were allele expression profile subset animals. Results Haplotypes H3 (21%) H1 (19%) most...
According to the protein-only hypothesis, infectious mammalian prions, which exist as distinct strains with discrete biological properties, consist of multichain assemblies misfolded cellular prion protein (PrP). A critical test would be produce synthetically from defined components. Crucially, high-titre ‘synthetic' prions could then used determine structural basis infectivity and strain diversity at atomic level. While there have been multiple reports production bacterially expressed...
There are currently few detailed studies describing HIV-1 recombination events or the potential impact of on drug resistance. We describe here viral dynamics in a drug-naive patient initially infected with circulating recombinant form 19 (CRF19) virus containing transmitted resistance mutations followed by superinfection "wild-type" subtype B virus. Single genome analysis showed replacement primary CRF19 recombinants and superinfecting The CRF19/B dominating after had lost at no time was...
Background: The SARS-CoV-2 Delta variant is highly transmissible and spreading globally but a detailed understanding of community transmission risks in vaccinated populations lacking.Methods: Between September 2020 August 2021, we recruited 510 contacts 422 UK COVID-19 cases to cohort study. A total 7194 upper respiratory tract (URT) samples were tested from sequential daily sampling participants for up 20 days. We analysed risk by vaccination status 139 exposed the variant. compared viral...
Abstract Background Cynomolgus macaques are indigenous to Asia occupying a range of geographical areas. A non‐indigenous population established on Mauritius approximately 500 years ago. Mauritian cynomolgus recognised as having low genetic diversity compared Indonesian macaques, from which they originated. As widely used biomedical model, there have been many studies their relationships. However, and relationships only assessed through analysis either the hypervariable region I or II...
<ns3:p><ns3:bold>Background:</ns3:bold> Lateral flow immunoassays (LFIAs) are able to achieve affordable, large scale antibody testing and provide rapid results without the support of central laboratories. As part development REACT programme extensive evaluation LFIA performance was undertaken with individuals following natural infection. Here we assess selected detect responses in who have received at least one dose severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)...
Hepatitis C virus (HCV) genotype (GT) 3 is the second most prevalent of seven HCV genotypes and exhibits greatest resistance to highly potent, direct-acting antivirals (DAAs) that are currently in use. Previously a stable cell line harbouring S52 GT3 sub-genomic replicon (SGR) was established, but this SGR unable robustly replicate transiently. As transient SGRs critical tool development DAAs, study viral resistance, we sought establish system based on S52. Next-generation sequencing used...
The relationship between gastrointestinal tract infection, the host immune response, and clinical outcome of disease is not well understood in COVID-19. We sought to understand effect intestinal responses SARS-CoV-2 on patient outcomes including magnitude systemic antibody induction. Combining two prospective cohort studies, International Severe Acute Respiratory emerging Infections Consortium Comprehensive Clinical Characterisations Collaboration (ISARIC4C) Integrated Network for...
The purpose of the present study was to formulate and systematically evaluate in vitro, ex vivo performances itraconazole containing bioadhesive vaginal film. We introduce here a novel intravaginal delivery system for based on solid dispersion hydroxypropyl methylcellulose E15 that improve drug solubility produce presence other ingredients. Solid dispersions were prepared with by spray drying method characterized X-ray diffraction differential scanning calorimetry. film using solvent...
The mouse-adapted scrapie prion strain RML is one of the most widely used in research. introduction a cell culture-based assay prions, (SCA) allows more rapid and precise titration. A semi-automated version this (ASCA) was applied to explore range conditions that might influence infectivity properties prions. These include resistance freeze-thaw procedures; stability endogenous proteases brain homogenate despite prolonged exposure varying temperatures; distribution infective material between...
<ns3:p><ns3:bold>Background:</ns3:bold> Lateral flow immunoassays (LFIAs) are able to achieve affordable, large scale antibody testing and provide rapid results without the support of central laboratories. As part development REACT programme extensive evaluation LFIA performance was undertaken with individuals following natural infection. Here we assess selected detect responses in who have received at least one dose severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)...
Abstract Background Lateral flow immunoassays (LFIAs) have the potential to deliver affordable, large scale antibody testing and provide rapid results without support of central laboratories. As part development REACT programme extensive evaluation LFIA performance was undertaken with individuals following natural infection. Here we assess selected detect responses in who received at least one dose SARS-CoV-2 vaccine. Methods This is a prospective diagnostic accuracy study. Setting Sampling...
ABSTRACT The Omicron variant of SARS-CoV-2 (B.1.1.529), first identified during November 2021, is rapidly spreading throughout the world, replacing previously dominant Delta variant. has a high number mutations in spike gene, some which are associated with greatly increased transmissibility and immune evasion. BA.1 sublineage been most prevalent but there recent evidence that BA.2 increasing proportion many countries. Genome sequencing gold standard for identification relatively slow,...