A5071258079- HIV Research and Treatment
- vaccines and immunoinformatics approaches
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Healthcare cost, quality, practices
- HIV/AIDS Research and Interventions
- Health Systems, Economic Evaluations, Quality of Life
- Monoclonal and Polyclonal Antibodies Research
- Clinical practice guidelines implementation
- HIV/AIDS drug development and treatment
- Cardiac Health and Mental Health
- Virus-based gene therapy research
- COVID-19 and Mental Health
- Cytomegalovirus and herpesvirus research
- Intensive Care Unit Cognitive Disorders
- Alzheimer's disease research and treatments
- T-cell and B-cell Immunology
- Pressure Ulcer Prevention and Management
- Prion Diseases and Protein Misfolding
- Neonatal skin health care
- Infection Control and Ventilation
- Biosensors and Analytical Detection
- Frailty in Older Adults
Imperial College London
2012-2025
Median SCP (Spain)
2025
International AIDS Vaccine Initiative
2024
Chelsea and Westminster Hospital NHS Foundation Trust
2022
University of Michigan
2010-2021
St Mary's Hospital
2021
International HIV/AIDS Alliance
2014-2021
VA Ann Arbor Healthcare System
2020-2021
Michigan Medicine
2014-2020
Geriatric Research Education and Clinical Center
2018-2020
Accurate antibody tests are essential to monitor the SARS-CoV-2 pandemic. Lateral flow immunoassays (LFIAs) can deliver testing at scale. However, reported performance varies, and sensitivity analyses have generally been conducted on serum from hospitalised patients. For use in community testing, evaluation of finger-prick self-tests, non-hospitalised individuals, is required.Sensitivity analysis was 276 participants. All had tested positive for by reverse transcription PCR were ≥21 days...
To evaluate the performance of new lateral flow immunoassays (LFIAs) suitable for use in a national coronavirus disease 2019 (covid-19) seroprevalence programme (real time assessment community transmission 2-React 2). Diagnostic accuracy study. Laboratory analyses were performed United Kingdom at Imperial College, London and university facilities London. Research clinics finger prick sampling run two affiliated NHS trusts. Sensitivity on sera stored from 320 previous participants React 2...
BI 1703880, a novel STimulator of INterferon Genes (STING) agonist, has demonstrated preclinical antitumor activity. As STING activation can upregulate programmed death ligand 1 and human leukocyte antigen in tumor cells, combination 1703880 an anti-programmed cell protein 1-antibody, such as ezabenlimab, may improve efficacy. This first-in-human phase Ia study (NCT05471856) is evaluating plus ezabenlimab patients with advanced solid tumors. The utilizes innovative lead-in design; all...
We conducted a phase I, randomized, double-blind, placebo-controlled trial to assess the safety and immunogenicity of escalating doses two recombinant replication defective adenovirus serotype 35 (Ad35) vectors containing gag, reverse transcriptase, integrase nef (Ad35-GRIN) env (Ad35-ENV), both derived from HIV-1 subtype A isolates. The enrolled 56 healthy HIV-uninfected adults.Ad35-GRIN/ENV (Ad35-GRIN Ad35-ENV mixed in same vial equal proportions) or Ad35-GRIN was administered...
Abstract It is currently unknown how post-COVID-19 syndrome (PCS) may affect those infected with severe acute respiratory coronavirus 2 (SARS-CoV-2). This longitudinal study includes healthcare staff who tested positive for SARS-CoV-2 between March and April 2020, follow-up of their antibody titers symptoms. More than half (21 38) had PCS after 7–8 months. There was no statistically significant difference initial reverse-transcription polymerase chain reaction or serial levels did not...
Even within the context of antiretroviral treatment and prevention, an HIV-1 vaccine remains best strategy for ending HIV/AIDS epidemic. A is particularly needed in sub-Saharan Africa, where greatly affects people's lives economy. Here, we aimed to assess safety immunogenicity candidate T-cell vaccines African populations. HIV-CORE 006 was a double-blind, randomised, placebo-controlled phase 1 trial conducted across four clinical research centres Uganda, Kenya, Zambia. Eligible participants...
Emergence of SIV and HIV specific CD8 T cells has been shown to correlate with control in vivo replication. Poor correlation between IFN-γ ELISPOT responses the virus triggered development more relevant assays assess functional HIV-1 T-cell for evaluation prioritization new vaccine candidates. We previously established a viral inhibition assay (VIA) that measures ability vaccine-induced inhibit replication autologous CD4 cells. In this assay, is determined by measuring p24 culture...
Abstract An LTR-based quantitative PCR (qPCR) assay was modified and optimized for the quantification of total HIV-1 nucleic acids in plasma PBMC. TaqMan qPCR primers probes were designed against NCBI/LANL compendium database by analyzing sequences used assays sensitive cross-clade detection as reported literature elucidating regions improved cross-subtype specificity. Inosine mixed nucleotide bases included at polymorphic sites. Real-time RT-qPCR performed on viral RNA cellular lysates. A...
Accurate and sensitive detection of antibody to SARS-CoV-2 remains an essential component the pandemic response. Measuring that predicts neutralising activity vaccine response is absolute requirement for laboratory-based confirmatory reference activity. The viral receptor binding domain (RBD) constitutes prime target antigen antibody. A double assay (DABA), providing most format has been exploited in a novel hybrid manner employing solid-phase S1 preferentially presenting RBD, coupled with...
The ability of HIV-1 vaccine candidates MRKAd5, VRC DNA/Ad5 and ALVAC/AIDSVAX to elicit CD8 T cells with direct antiviral function was assessed compared HIV-1-infected volunteers.Adenovirus serotype 5 (Ad5)-based regimens MRKAd5 DNA/Ad5, designed HIV-1-specific cells, are immunogenic but failed prevent infection or impact on viral loads in volunteers infected subsequently. Failure may be due part a lack effective functions.An in-vitro inhibition assay tested the bispecific antibody expanded...
The purposes of this retrospective study are to: 1) describe the amount and type documented mobility activity for patients with an indwelling Pulmonary Artery Catheter (PAC) 2) document frequency complications that occur PAC during patient participation in these activities.This is a single-center, retrospective, descriptive including all (>18 years) between June 2010 October 2012 Cardiology Intensive Care Unit (CICU). Data was extracted on each performed nursing or skilled treatments...
Critical care workers were considered to be at high risk of severe acute respiratory syndrome coronavirus-2 infection from patients during the first wave pandemic. Staff symptoms, previous swab testing, and antibody prevalence correlated with patient admissions investigate this assumption.Cross-sectional study.A large critical department in a tertiary-care teaching hospital London, United Kingdom.Staff working care.None.Participants completed questionnaire provided serum sample for testing...
Background: The world needs an effective HIV-1/AIDS vaccine. Even in the broader context of antiretroviral treatment and prevention, a vaccine remains best solution likely key component any package truly ending AIDS epidemic. Vaccine is particularly needed most affected regions like sub-Saharan Africa, where HIV-1 significantly impacts people's lives economy.Methods: CD8+ killer T cells impose selective pressure on their protective potential should be utilized for vaccination. Our program...
Purpose: Scores on the Activity Measure for Post-Acute Care (AMPAC) 6-Clicks are used in hospital setting as functional measures to guide care and discharge recommendations. However, limited evidence exists regarding accuracy of AMPAC predicting location those with cardiovascular disease (CVD). The purpose this study was determine AM-PAC scores predict home/self-care from an acute adults CVD. Methods: A retrospective review medical record data comprised basic mobility or daily activity at...
Full characterisation of functional HIV-1-specific T-cell responses, including identification recognised epitopes linked with antiviral would aid development effective vaccines but is hampered by HIV-1 sequence diversity. Typical approaches to identify utilising extensive peptide sets require subjects’ cell numbers that exceed feasible sample volumes. To address this, CD8 T-cells were polyclonally expanded from PBMC 13 anti-retroviral naïve subjects living HIV using CD3/CD4 bi-specific...
Introduction Immunological protection against human immunodeficiency virus-1 (HIV-1) infection is likely to require both humoral and cell-mediated immune responses, the latter involving cytotoxic CD8 T-cells. Characterisation of T-cell mediated direct anti-viral activity would provide understanding potential correlates identification critical epitopes associated with HIV-1 control. Methods The present report describes a functional viral inhibition assay (VIA) assess T-cell-mediated...
T cells play an important role in controlling viral replication during HIV infection. An effective vaccine should, therefore, lead to the induction of a strong and early viral-specific CD8+ cell response. While polyfunctional responses are thought be contributors antiviral response, there is evidence show that HIV- specific just small fraction total HIV-specific may absent many individuals who control replication, suggesting other HIV-1 effector subsets key players control. Stem cell-like...
Background & Purpose: Research shows that early mobilization of critically ill patients is safe and effective in shortening length stay improving outcomes. We describe our experience with a patient following total artificial heart (TAH) surgery since limited guidance regarding physical therapy (PT) management available. Case Presentation: A 49-year-old was admitted for left ventricular assist device versus transplant evaluation underwent TAH using CardioWest Total Artificial Heart. Physical...
ABSTRACT The ability of vaccine-induced T cells to inhibit viral replication may contribute protect against human immunodeficiency virus (HIV) acquisition. Here, we tested ex vivo inhibitory activity cell responses induced by a multivalent HIV vaccine based on the replication-incompetent recombinant adenovirus serotype 26 vector with mosaic immunogen strategy (Ad26.Mos.HIV), designed for broad immune coverage diverse HIV-1 strains. Using clinical trial samples range measured IFN-γ ELISpot,...
INTRODUCTION/CLINICAL RELEVANCE: Cardiovascular disease (CVD) effects mobility which could lead to prolonged hospitalization and increased healthcare costs. Measuring function using Activity Measure for Post-Acute Care 6-click (AMPAC) scores can accurately predict acute hospital discharge location but there is limited research in patients with CVD. The purpose of this study was describe the accuracy AMPAC’s daily activity destination from an care hospital. METHODS: A retrospective review...
Background: Accurate and sensitive detection of antibody to SARS-CoV-2 remains an essential component the pandemic response. Measuring that predicts neutralising activity vaccine response is absolute requirement for laboratory-based confirmatory reference activity.Methods: The viral receptor binding domain (RBD) constitutes prime target antigen antibody. A double assay (DABA) provides most format. It has been exploited in a novel hybrid manner employing S1 solid-phase preferentially...