A5056093130- Cardiac electrophysiology and arrhythmias
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Acute Myocardial Infarction Research
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- COVID-19 Clinical Research Studies
- Congenital heart defects research
- Neuroscience and Neural Engineering
- Venous Thromboembolism Diagnosis and Management
- Atrial Fibrillation Management and Outcomes
- Angiogenesis and VEGF in Cancer
- Long-Term Effects of COVID-19
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Biomarkers in Disease Mechanisms
- COVID-19 and healthcare impacts
- Cardiac Imaging and Diagnostics
- Extracellular vesicles in disease
- Coronary Interventions and Diagnostics
- Cardiomyopathy and Myosin Studies
- SARS-CoV-2 and COVID-19 Research
- Cardiac Ischemia and Reperfusion
- Inflammatory Biomarkers in Disease Prognosis
- 3D Printing in Biomedical Research
University of Pavia
2016-2025
Policlinico San Matteo Fondazione
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2025
University of Padua
2023-2024
IRCCS Istituto Auxologico Italiano
2023-2024
Epatocentro Ticino
2023-2024
Ospedale San Giovanni Bosco
2023-2024
University of Turin
2023
Instituto de Investigación Biomédica de Salamanca
2023
Ospedale SS. Annunziata
2023
We previously reported that intramyocardial injection of bone marrow-derived mesenchymal stem cells overexpressing Akt (Akt-MSCs) inhibits ventricular remodeling and restores cardiac function measured 2 wk after myocardial infarction. Here, we report the functional improvement occurs in < 72 h. This early remarkable effect cannot be readily attributed to regeneration from donor cells. Thus, hypothesized paracrine actions exerted by through release soluble factors might important mechanisms...
Abstract Aims To evaluate the impact of COVID-19 pandemic on patient admissions to Italian cardiac care units (CCUs). Methods and Results We conducted a multicentre, observational, nationwide survey collect data for acute myocardial infarction (AMI) at CCUs throughout 1 week period during outbreak, compared with equivalent in 2019. observed 48.4% reduction AMI 2019 (P &lt; 0.001). The was significant both ST-segment elevation [STEMI; 26.5%, 95% confidence interval (CI) 21.7–32.3; P =...
Stem cell therapy has emerged as a promising tool for the treatment of variety diseases. Previously, we have shown that Akt-modified mesenchymal stem cells mediate tissue repair through paracrine mechanisms. Using comprehensive functional genomic strategy, show secreted frizzled related protein 2 (Sfrp2) is key factor mediates myocardial survival and after ischemic injury. Sfrp2 known to modulate Wnt signaling, demonstrate cardiomyocytes treated with increase cellular beta-catenin...
We previously reported that intramyocardial injection of bone marrow-derived mesenchymal stem cells overexpressing Akt (MSC-Akt) efficiently repaired infarcted rat myocardium and improved cardiac function. Controversy still exists over the mechanisms by which MSC contribute to tissue repair. Herein, we tested if cellular fusion plays a determinant role in injected expressing Cre recombinase, with or without Akt, into reporter mice. In these mice, LacZ is expressed only after Cre-mediated...
<h3>Importance</h3> Myocardial injury, detected by elevated plasma troponin levels, has been associated with mortality in patients hospitalized coronavirus disease 2019 (COVID-19). However, the initial data were reported from single-center or 2-center studies Chinese populations. Compared these patients, European and US are older, more comorbidities higher rates. <h3>Objective</h3> To evaluate prevalence prognostic value of myocardial a large population White Italian COVID-19. <h3>Design,...
Abstract Long COVID has become a world-wide, non-communicable epidemic, caused by long-lasting multiorgan symptoms that endure for weeks or months after SARS-CoV-2 infection already subsided. This scientific document aims to provide insight into the possible causes and therapeutic options available cardiovascular manifestations of long COVID. In addition chronic fatigue, which is common symptom COVID, patients may present with chest pain, ECG abnormalities, postural orthostatic tachycardia,...
The existence of circulating endothelial progenitor cells (CEPCs) has previously been documented. These can be mobilized by cytokines and are recruited to sites injury, where they may participate in tissue repair. In the present study, we examined hypothesis that mobilization CEPCs exogenous granulocyte-colony stimulating factor (G-CSF) enhances repair injured arteries facilitating reendothelialization inhibiting neointima development.Male rats were injected daily with 50 microg/kg...
Vagal activity has protective effects in ischemic heart disease. We tested whether vagal stimulation (VS) could modulate the inflammatory reaction, a major determinant of cardiac injury after ischemia/reperfusion. Four groups male rats underwent myocardial ischemia (30 minutes) and reperfusion (24 hours). One group VS (40 minutes), 1 plus atrial pacing (VS + Pacing), nicotinic inhibition by mecamylamine MEC). After 24 hours, area at risk, infarct size, inflammation parameters, apoptosis were...
Loss-of-function mutations in the hERG gene causes long-QT syndrome type 2 (LQT2), a condition associated with reduced IKr current. Four different mutation classes define molecular mechanisms impairing hERG. Among them, Class determine trafficking defects. Lumacaftor (LUM) is drug acting on channel already successfully tested for cystic fibrosis and its safety profile well known. We hypothesize that LUM might rescue also defects LQT2 exert anti-arrhythmic effects. From five patients, we...
Calmodulin (CaM) is a small protein, encoded by three genes (CALM1-3), exerting multiple Ca2+-dependent modulatory roles. A mutation (F142L) affecting only one of the six CALM alleles associated with long QT syndrome (LQTS) characterized recurrent cardiac arrests. This phenotypic severity unexpected from predicted allelic balance. In this work, effects heterozygous CALM1-F142L have been investigated in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) obtained LQTS...
Abstract The paracrine properties of human amniotic membrane-derived mesenchymal stromal cells (hAMCs) have not been fully elucidated. goal the present study was to elucidate whether hAMCs can exert beneficial effects on infarcted rat hearts, in particular through cardioprotection and angiogenesis. Moreover, we aimed identify putative active mediators. were isolated, expanded, characterized. In vitro, conditioned medium from hAMC (hAMC-CM) exhibited cytoprotective proangiogenic properties....
Abstract Aims Having shown that Lumacaftor rescued the hERG trafficking defect in induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) of two LQT2 patients, we tested whether commercial association + Ivacaftor (LUM IVA) could shorten QTc same patients. Methods and results After hospital admission 1 day baseline recordings, half dose LUM IVA was administered on Day 1, followed by full 800 mg 500 mg) for 7 days. A continuous 12-lead Holter ECG allowed a large number blind...
The release of neutrophil extracellular traps (NETs), a process termed NETosis, avoids pathogen spread but may cause tissue injury. NETs have been found in severe COVID-19 patients, their role disease development is still unknown. aim this study to assess the capacity drive epithelial-mesenchymal transition (EMT) lung epithelial cells and analyze involvement COVID-19. Bronchoalveolar lavage fluid patients showed high concentration that correlates with neutrophils count; moreover, analysis...
We reported previously that predelivery of the anti-oxidant gene heme oxygenase-1 (HO-1) to heart by adeno associated virus (AAV) markedly reduces injury after acute myocardial infarction (MI). However, effect HO-1 delivery on postinfarction recovery has not been investigated. In current study, we assessed post-MI left ventricle (LV) remodeling and function using echocardiographic imaging histomorphometric approaches. Two groups Sprague-Dawley rats were injected with 4 x 10(11) particles...
Abstract Administration of mesenchymal stem cells (MSCs) is an effective therapy to repair cardiac damage after myocardial infarction (MI) in experimental models. However, the mechanisms action still need be elucidated. Our group has recently suggested that MSCs mediate their therapeutic effects primarily via paracrine cytoprotective action. Furthermore, we have shown overexpressing Akt1 (Akt-MSCs) exert even greater cytoprotection than unmodified MSCs. So far, little been reported on...
Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death in world. The multikinase inhibitor sorafenib only demonstrated marginal improvement overall survival for advanced disease prompted search alternative treatment options. Human mesenchymal stem cells (MSCs) have ability to home tumor cells. However, its functional roles on microenvironment remain controversial. Herein, we showed that conditioned media derived from human fetal MSC (CM-hfMSCs) expressed high level...