A5044313291- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- Toxoplasma gondii Research Studies
- Trauma and Emergency Care Studies
- Malaria Research and Control
- Biochemical and Molecular Research
- Tuberculosis Research and Epidemiology
- Metabolomics and Mass Spectrometry Studies
- Emergency and Acute Care Studies
- Posttraumatic Stress Disorder Research
- Migration, Health and Trauma
- Monoclonal and Polyclonal Antibodies Research
- Synthesis and Catalytic Reactions
- Biotin and Related Studies
- Grief, Bereavement, and Mental Health
- Cannabis and Cannabinoid Research
- Forest ecology and management
- Polyamine Metabolism and Applications
- Sex work and related issues
- Allelopathy and phytotoxic interactions
- Cultural Competency in Health Care
- Sexual Assault and Victimization Studies
- Microbial Natural Products and Biosynthesis
- Innovative Human-Technology Interaction
University of Michigan
2017-2023
Stanford University
2019-2022
ORCID
2022
Terre des Hommes
2022
Michigan Medicine
2022
Haverford College
2018
VA Center for Clinical Management Research
2017
VA Ann Arbor Healthcare System
2017
Brunswick (United States)
1975-1977
Salinipostin A (Sal A) is a potent antiplasmodial marine natural product with an undefined mechanism of action. Using Sal A-derived activity-based probe, we identify its targets in the Plasmodium falciparum parasite. All identified proteins contain α/β serine hydrolase domains and several are essential for parasite growth. One displays high degree homology to human monoacylglycerol lipase (MAGL) able process lipid esters including MAGL acylglyceride substrate. This target inhibited by...
The N-acyl amino acids are a family of bioactive lipids with pleiotropic physiologic functions, including in energy homeostasis. Their endogenous levels regulated by an extracellular mammalian acid synthase/hydrolase called PM20D1 (peptidase M20 domain containing 1). Using activity-guided biochemical approach, we report the molecular identification fatty amide hydrolase (FAAH) as second intracellular synthase/hydrolase. In vitro, FAAH exhibits more restricted substrate scope compared to...
Scientific outreach efforts traditionally involve formally trained scientists teaching the general public about methods, significance, and excitement of science. We recently experimented with an alternative "symbiotic outreach" model that prioritizes building a reciprocal relationship between "outsider" to facilitate active two-way communication. Herein, we present results our effort involving college students adults intellectual developmental disabilities working together make biological...
ABSTRACT Secreted polypeptides are a fundamental biochemical axis of intercellular and endocrine communication. However, global understanding composition dynamics cellular secretomes in intact mammalian organisms has been lacking. Here, we introduce proximity biotinylation strategy that enables labeling, detection, enrichment secreted cell type-selective manner mice. We generate proteomic atlas hepatocyte, myocyte, pericyte, myeloid by direct purification biotinylated from blood. Our...
Bovine respiratory disease remains the leading cause of feedlot morbidity and mortality in North America. The objective this study was to evaluate effect generic vs. pioneer tulathromycin for control bovine disease.
Summary The increasing incidence of antibiotic-resistant Mycobacterium tuberculosis infections is a global health threat necessitating the development new antibiotics. Serine hydrolases (SHs) are promising class targets because their importance for synthesis mycobacterial cell envelope. We screened library small molecules containing serine-reactive electrophiles and identified narrow spectrum inhibitors M. tuberculous growth. Using these lead molecules, we performed competitive...
Abstract The mortality of pine seedlings caused by seymeria [Seymeria cassioides (J. F. Gmelin) Blake] infestations has been documented. results our study in Georgia substantiate the suggestions previous authors that growth losses occur infested surviving attack and increment extend into following growing season.
Our work focuses on the discovery of new biosynthetic routes to chemical diversity through characterization unexplored polyketide synthase (PKS) gene clusters and enzymes from ancient diverse phyla. In first part this work, two non‐actinobacterial organisms were cultured, their secondary metabolites extracted, fractionated, characterized by liquid chromatography mass spectrometry (LC‐MS) in search for novel molecules pathways. These data are currently being compared existing molecular...
SUMMARY Salinipostin A (Sal A) is a potent antimalarial marine natural product with an undefined mechanism of action. Using Sal A-derived activity-based probe, we identify its targets in the Plasmodium falciparum parasite. All identified proteins contain α/β serine hydrolase domains, and several are essential for parasite growth. One displays high homology to human monoacylglycerol lipase (MAGL) able process lipid esters including MAGL acylglyceride substrate. This target inhibited by...
Salinipostin A (Sal A) is a potent antimalarial marine natural product with an undefined mechanism of action. Using Sal A-derived activity-based probe, we identify its targets in the Plasmodium falciparum parasite. All identified proteins contain α/β serine hydrolase domains and several are essential for parasite growth. One displays high homology to human monoacylglycerol lipase (MAGL) able process lipid esters including MAGL acylglyceride substrate. This target inhibited by anti-obesity...
Summary The intracellular protozoan parasite Toxoplasma gondii must scavenge cholesterol and other lipids from the host to facilitate growth replication. Enzymes responsible for neutral lipid synthesis have been identified but there is no evidence enzymes that catalyze lipolysis of esters esterified lipids. Here we characterize several T. serine hydrolases with esterase thioesterase activities were previously thought be depalmitoylating enzymes. We find they do not cleave palmitoyl thiol...
The intracellular protozoan parasite Toxoplasma gondii must scavenge cholesterol and other lipids from the host to facilitate growth replication. Enzymes responsible for neutral lipid synthesis have been identified but there is no evidence enzymes that catalyze lipolysis of esters esterified lipids. Here we characterize several T. serine hydrolases with esterase thioesterase activities were previously thought be depalmitoylating enzymes. We find they do not cleave palmitoyl thiol rather...
INTRODUCTION: Most people have experienced assault, an adverse childhood event, or other trauma. Trauma adversely impacts reproductive health outcomes and patients' experiences of care. Survivors' preferences for trauma-informed pelvic care are established in the literature, but there scant data on clinicians' adherence to these practices. This study assessed knowledge, attitudes, practices regarding (TIC) examinations. METHODS: The team distributed anonymous Qualtrics survey 1,017...