A5054945716- Carcinogens and Genotoxicity Assessment
- Advanced Chemical Sensor Technologies
- Hormonal and reproductive studies
- Air Quality and Health Impacts
- Indoor Air Quality and Microbial Exposure
- Effects and risks of endocrine disrupting chemicals
- Molecular Biology Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Veterinary Equine Medical Research
- Reproductive Physiology in Livestock
- Pharmacological Effects and Assays
- Muscle metabolism and nutrition
- Pharmacogenetics and Drug Metabolism
- Antioxidant Activity and Oxidative Stress
- Odor and Emission Control Technologies
- Smoking Behavior and Cessation
- Olfactory and Sensory Function Studies
- Pharmacology and Obesity Treatment
- Pesticide Exposure and Toxicity
- DNA Repair Mechanisms
- Forensic Toxicology and Drug Analysis
- Plant Toxicity and Pharmacological Properties
- Veterinary Pharmacology and Anesthesia
- Adipose Tissue and Metabolism
- Hypothalamic control of reproductive hormones
University of California, Davis
2012-2024
Mental Research Institute
2021
California Department of Food and Agriculture
2021
Lovelace Respiratory Research Institute
2013-2020
University of North Carolina at Chapel Hill
2010-2017
Northwestern Medicine
2014
Cancer Genetics (United States)
2014
West Health
2012
IVI Madrid Clinic
2010
Poznan University of Medical Sciences
2010
The beneficial outcome associated with the use of proton pump inhibitors (PPIs) in idiopathic pulmonary fibrosis (IPF) has been reported retrospective studies. To date, no prospective study conducted to confirm these outcomes. In addition, potential mechanism by which PPIs improve measures lung function and/or transplant-free survival IPF not elucidated.Here, we used biochemical, cell biological and preclinical studies evaluate regulation markers inflammation fibrosis. our vitro studies,...
The presence of endogenous and exogenous N(2)-hydroxymethyl-dG adducts in DNA from the nasal mucosa bone marrow cynomolgus macaques exposed to 1.9 6.1 ppm [(13)CD(2)]-formaldehyde for 6 h a day 2 consecutive days was investigated using highly sensitive nano-UPLC-MS/MS method with limit detection 20 amol. Both were readily detected quantified tissues both exposure groups, an dependent increase observed. In contrast, only detectable marrow, even though ∼10 times more analyzed.
Formaldehyde is not only a widely used chemical with well-known carcinogenicity but also normal metabolite of living cells. It thus poses unique challenges for understanding risks associated exposure. N2-hydroxymethyl-dG (N2-HOMe-dG) the main formaldehyde-induced DNA mono-adduct, which together DNA-protein crosslinks (DPCs) and toxicity-induced cell proliferation, play important roles in mutagenic mode action cancer. In this study, N2-HOMe-dG was shown to be an excellent biomarker direct...
DNA-protein crosslinks (DPC) arise from a wide range of endogenous and exogenous chemicals, such as chemotherapeutic drugs formaldehyde. Importantly, recent identification aldehydes genotoxins in Fanconi anemia has provided new insight into disease causation. Because their bulky nature, DPCs pose severe threats to genome stability, but previous methods measure formaldehyde-induced were incapable discriminating between sources chemical. In this study, we developed that provide accurate...
In this study, both endogenous and exogenous N2-hydroxymethyl-dG adducts in nasal DNA of rats exposed to 0.7, 2, 5.8, 9.1, or 15.2 ppm [13CD2] formaldehyde for 6 h were quantified by a highly sensitive nano-UPLC-MS/MS method. Our data clearly demonstrated that form nonlinear fashion, with 21.7-fold increase exposure causing 286-fold adducts. The ratio exogenous/endogenous dominated at low exposures, comprising more than 99%. contrast, not detectable the bone marrow formaldehyde.
Significance We have characterized the relative bioactivity of physiological progestin, dihydroprogesterone (DHP), showing in vivo it maintains equine gestation absence progesterone, generally believed only steroid capable supporting pregnancy. DHP activates progesterone receptor (PR) as potently itself, and human PR enough to suggest support for Thus, joins this class other naturally synthesized progestin so defined. This settles five decades speculation that sustains pregnancies second...
MicroRNAs (miRNAs) are critical regulators of gene expression, yet much remains unknown regarding their changes resulting from environmental exposures as they influence cellular signaling across various tissues. We set out to investigate miRNA responses formaldehyde, a air pollutant and known carcinogen that disrupts expression profiles. Rats were exposed by inhalation either 0 or 2 ppm formaldehyde for 7, 28, 28 days followed 7-day recovery. Genome-wide profiles assessed within the nasal...
Summary Reasons for performing the study: Increased plasma progestagen concentrations have been reported in foals with neonatal maladjustment syndrome (NMS). These steroids may cross blood–brain barrier and dampening effects central nervous system. Objectives: To evaluate if infusion of a progesterone derivative (allopregnanolone) healthy foal would induce clinical signs compatible NMS. Methods: A from mare normal gestation (length, no complications), birth placenta was infused...
Formaldehyde is an air pollutant present in both indoor and outdoor atmospheres. Because of its ubiquitous nature, it imperative to understand the mechanisms underlying formaldehyde-induced toxicity carcinogenicity. MicroRNAs (miRNAs) can influence disease caused by environmental exposures, yet miRNAs are understudied relation formaldehyde. Our previous investigation demonstrated that formaldehyde exposure human lung cells disruptions miRNA expression profiles vitro.Using vivo model, we set...
Formaldehyde is a known human and animal carcinogen that forms DNA adducts, causes mutations. While there widespread exposure to formaldehyde in the environment, also an essential biochemical all living cells. The presence of both endogenous exogenous sources makes it difficult develop exposure-specific biomarkers. Furthermore, chemicals such as nitrosodimethylamine form one mole for every methylating agent, raising questions about potential cocarcinogenesis. Formaldehyde-induced...
With formaldehyde as the major source of endogenous N⁶-formyllysine protein adducts, we quantified and exogenous in nasal epithelium rats exposed by inhalation to 0.7, 2, 5.8, 9.1 ppm [¹³C²H₂]-formaldehyde using liquid chromatography-coupled tandem mass spectrometry. Exogenous was detected epithelium, with concentration-dependent formation total well fractionated (cytoplasmic, membrane, nuclear) proteins, but not lung, liver, or bone marrow. Endogenous adducts dominated at all exposure...
The dose-response relationship for biomarkers of exposure (N2-ethylidene-dG adducts) and effect (cell survival micronucleus formation) was determined across 4.5 orders magnitude (50nM–2mM) using [13C2]-acetaldehyde exposures to human lymphoblastoid TK6 cells 12h. There a clear increase in exogenous N2-ethylidene-dG formation at concentrations ≥ 1µM, whereas the endogenous adducts remained nearly constant all concentrations, with an average 3.0 adducts/107 dG. Exogenous were lower than ≤ 10µM...
The adverse health effects of environmental exposure to gaseous and particulate components vehicular emissions are a major concern among urban populations. A link has been established between respiratory the development cardiovascular disease (CVD), but mechanisms driving this interaction remain unknown. Chronic inhalation mixed vehicle linked CVD in animal models. This study evaluated temporal acute (MVE; gasoline diesel emissions) on potentially active metabolites serum exposed mice....
More and more studies are reporting on the natural transmission of SARS-CoV-2 between humans with COVID-19 their companion animals (dogs cats). While horses apparently susceptible to infection based homology human equine ACE-2 receptor, no clinical or subclinical has yet been reported in species. To investigate possible role equids, nasal secretions from 667 acute onset fever respiratory signs were tested for presence by qPCR. The samples collected January December 2020 submitted a...
Methanol is a large volume industrial chemical and widely used solvent fuel additive. Methanol's well known toxicity use in wide spectrum of applications has raised long-standing environmental issues over its safety, including carcinogenicity. not been listed as carcinogen by any regulatory agency; however, there are debates about carcinogenic potential. Formaldehyde, metabolite methanol, proposed to be responsible for the carcinogenesis methanol. Formaldehyde actively targets DNA protein,...
The Association of Official Racing Chemists (AORC) guidelines for drug testing in animal hair provide sport doping control laboratories with a framework the implementation robust and legally defensible program analysis, both screening confirmatory, samples. were compiled by AORC Hair Analysis Committee, which is comprised experts from around world, before being ratified membership. They guidance on all stages sample collection, through pre-treatment extraction onto instrumental analysis.
This study investigated adrenal androgens (AA), gonadotropins, and cortisol in castrated gonad-intact male rhesus macaques from birth through infancy. Blood samples were collected longitudinally (n = 6; weekly, 1-40 wk) intact 4; every other week, 1-17 males. Plasma concentrations of AA determined by liquid chromatography-tandem mass spectrometry, plasma gonadotropins RIA. Dehydroepiandrosterone sulfate (DHEAS) increased almost threefold (to 8 wk), dehydroepiandrosterone (DHEA) more than...