A5018526439- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Diet, Metabolism, and Disease
- Hedgehog Signaling Pathway Studies
- Hepatitis C virus research
- Liver Disease and Transplantation
- Organ Transplantation Techniques and Outcomes
- Liver Diseases and Immunity
- Hepatitis B Virus Studies
- Pancreatic function and diabetes
- Genetic and Kidney Cyst Diseases
- Alcohol Consumption and Health Effects
- Drug-Induced Hepatotoxicity and Protection
- Digestive system and related health
- Dietary Effects on Health
- Bone and Dental Protein Studies
- Thyroid Disorders and Treatments
- Drug-Induced Adverse Reactions
- Religion, Spirituality, and Psychology
- Renal and related cancers
- Mitochondrial Function and Pathology
- Hops Chemistry and Applications
- Lipid metabolism and disorders
- thermodynamics and calorimetric analyses
- Genetic Neurodegenerative Diseases
Saint Louis University
2023-2025
University of the Basque Country
2011-2025
Medical University of South Carolina
2017-2023
Ralph H. Johnson VA Medical Center
2017-2022
Indiana University – Purdue University Indianapolis
2019
Foundation for Liver Research
2012-2017
Barts Health NHS Trust
2014-2015
Duke University
2009-2015
Duke Medical Center
2010-2014
Duke University Hospital
2012-2014
Liver inflammation is greater in nonalcoholic steatohepatitis (NASH) than steatosis, suggesting that immune responses contribute to fatty liver disease (NAFLD) progression. Livers normally contain many natural killer T (NKT) cells produce factors modulate inflammatory and fibrogenic responses. Such are relatively depleted but their status more advanced NAFLD uncertain. We hypothesized NKT accumulate promote fibrosis progression NASH. aimed determine if livers become enriched with during...
Nonalcoholic steatohepatitis (NASH) is a leading cause of cirrhosis. Recently, we showed that NASH-related cirrhosis associated with Hedgehog (Hh) pathway activation. The gene encoding osteopontin (OPN), profibrogenic extracellular matrix protein and cytokine, direct transcriptional target the Hh pathway. Thus, hypothesize signaling induces OPN to promote liver fibrosis in NASH. Hepatic expression were analyzed wild-type (WT) mice, Patched-deficient (Ptc+/−) (overly active signaling)...
<h3>Objective</h3> Immune responses are important in dictating non-alcoholic steatohepatitis (NASH) outcome. We previously reported that upregulation of hedgehog (Hh) and osteopontin (OPN) occurs NASH, Hh-regulated accumulation natural killer T (NKT) cells promotes hepatic stellate cell (HSC) activation, cirrhotic livers harbour large numbers NKT cells. <h3>Design</h3> The hypothesis activated drive fibrogenesis during NASH was evaluated by assessing if depletion protects against fibrosis;...
Objective Chronic fibrosing liver injury is a major risk factor for hepatocarcinogenesis in humans. Mice with targeted deletion of Mdr2 (the murine ortholog MDR3) develop chronic injury. Hepatocellular carcinoma (HCC) emerges spontaneously such mice by 50–60 weeks age, providing model fibrosis-associated hepatocarcinogenesis. We used Mdr2−/− to investigate the hypothesis that activation hedgehog (Hh) signaling pathway promotes development both fibrosis and HCC. Methods Hepatic fibrosis, Hh...
<h3>Objective</h3> Vascular remodelling during liver damage involves loss of healthy sinusoidal endothelial cell (LSEC) phenotype via capillarisation. Hedgehog (Hh) signalling regulates vascular development and increases injury. This study therefore examined its role in <h3>Design</h3> Primary LSEC were cultured for 5 days to induce Pharmacological, antibody-mediated genetic approaches used manipulate Hh signalling. Effects on mRNA protein expression Hh-regulated genes capillarisation...
Smoothened (SMO), a coreceptor of the Hedgehog (Hh) pathway, promotes fibrogenic repair chronic liver injury. We investigated roles SMO+ myofibroblast (MF) in regeneration by conditional deletion SMO α smooth muscle actin (αSMA)+ cells after partial hepatectomy (PH).αSMA-Cre-ER(T2)×SMO/flox mice were treated with vehicle (VEH) or tamoxifen (TMX), and sacrificed 24-96 h post-PH. Regenerating livers analysed for proliferation, progenitors fibrosis qRT-PCR quantitative immunohistochemistry...
Hepatocellular carcinoma (HCC) typically develops in cirrhosis, a condition characterized by Hedgehog (Hh) pathway activation and accumulation of Hh-responsive myofibroblasts. Although Hh signaling generally regulates stromal-epithelial interactions that support epithelial viability, the role Hh-dependent myofibroblasts hepatocarcinogenesis is unknown. Here, we used human HCC samples, mouse model, hepatoma cell/myofibroblast cocultures to examine hypothesis modulates myofibroblasts'...
Noninvasive biomarkers are clinically useful for evaluating liver fibrosis stage in patients with nonalcoholic fatty disease (NAFLD). The aim of the present study was to compare plasma proteins early steatohepatitis (NASH) (F0-F1) versus NASH significant/advanced (F2-F4) determine whether candidate could be used as potential noninvasive biomarkers. Nineteen biopsy-proven NAFLD including ten and nine were enrolled study. High-resolution proteomics screening performed SCIEX TripleTOF 5600...
Background and Aims: Non-alcoholic beers (NABs) are gaining popularity as alternatives to alcoholic beverages, yet their metabolic health effects compared no consumption of these drinks remain unclear. Material Methods: The investigator-blinded, single-center, randomized study compares the on metabolism, health, gut microbiome daily different NABs—pilsener, mixed beer, wheat beer—on glucose fat body composition, liver function in 44 healthy young men. participants consumed 660 mL one or...
Background/Aims Emerging data links different aspects of lipid metabolism to liver regeneration. In patients with acute failure (ALF), low levels lipids may correlate disease severity. Thus, we determined whether there is an etiology-specific link between in suffering from ALF and aimed investigate effect on the prognosis ALF. Methods this retrospective single center study, reviewed 89 consecutive patients, who met criteria "Acute Liver Failure Study Group". Patient characteristics, clinical...
Despite advances in management, bladder cancer remains a major cause of related complications. Characterisation gene expression patterns allows the identification pathways involved its pathogenesis, and may stimulate development novel therapies targeting these pathways. Between 2004 2005, cystoscopic biopsies were obtained from 19 patients 11 controls. These subjected to whole transcript-based microarray analysis. Unsupervised hierarchical clustering was used identify samples with similar...
OPN (osteopontin)) is a Hh (Hedgehog)-regulated cytokine that up-regulated during chronic liver injury and directly promotes fibrosis. We have reported signalling enhances viral permissiveness replication in HCV (hepatitis C virus)-infected cells. Hence we hypothesized replication, targeting could be beneficial HCV. In the present study, compared expression of mRNA protein (JFH1)-infected Huh7 Huh7.5 cells, evaluated whether modulating levels using exogenous ligands (up-regulate OPN) or...