A5079669538- Meteorological Phenomena and Simulations
- Precipitation Measurement and Analysis
- Erythrocyte Function and Pathophysiology
- Epigenetics and DNA Methylation
- Hemoglobinopathies and Related Disorders
- RNA modifications and cancer
- Climate variability and models
- Virus-based gene therapy research
- Genomics and Chromatin Dynamics
- Soil Moisture and Remote Sensing
- Atmospheric aerosols and clouds
- Acute Myeloid Leukemia Research
- CRISPR and Genetic Engineering
- Geophysics and Gravity Measurements
- Ionosphere and magnetosphere dynamics
- Single-cell and spatial transcriptomics
- Hematopoietic Stem Cell Transplantation
- Tropical and Extratropical Cyclones Research
- RNA Interference and Gene Delivery
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- RNA Research and Splicing
- Kruppel-like factors research
- Radio Wave Propagation Studies
- Flood Risk Assessment and Management
- Animal Genetics and Reproduction
University of Oklahoma
2016-2025
Radar (United States)
2016-2025
National Human Genome Research Institute
2015-2024
Applied Radar (United States)
2023
National Institutes of Health
2011-2022
NOAA National Severe Storms Laboratory
2015-2022
NOAA National Weather Service
2022
University of Alabama in Huntsville
2022
Kyoto University
2020-2022
Cooperative Institute for Mesoscale Meteorological Studies
2020
Moloney Murine Leukemia Virus (MoMuLV) causes T cell neoplasms in rodents but is not known to be a pathogen primates. The core protein and enzyme genes of the MoMuLV genome together with an amphotropic envelope gene are utilized engineer lines that generate retroviral vectors for use current human therapy applications. We developed producer clone generates very high concentration vector particles optimize conditions insertion into pluripotent hematopoietic stem cells. This line also much...
ABSTRACT We investigated the function of Lhx2, a LIM homeobox gene expressed in developing B-cells, forebrain and neural retina, by analyzing embryos deficient functional Lhx2 protein. mutant are anophthalmic, have malformations cerebral cortex, die utero due to severe anemia. In Lhx2−/− specification optic vesicle occurs; however, development eye arrests prior formation an cup. Deficient cellular proliferation results hypoplasia neocortex aplasia hippocampal anlagen. addition central...
Interleukin 6 (IL-6) is an important regulator of the acute phase response, T cell function, and terminal B differentiation. Excessive or inappropriate production this cytokine may be involved in a variety autoimmune neoplastic disorders. To investigate consequences dysregulated synthesis IL-6 vivo, high-titer recombinant retroviral vector produced psi-2 packaging cells was used to introduce coding sequences murine into mouse hematopoietic cells. Congenitally anemic W/Wv mice reconstituted...
The mechanisms that regulate hematopoietic stem cell (HSC) fate decisions between proliferation and multilineage differentiation are unclear. Members of the Wnt family ligands activate canonical signaling pathway, which utilizes beta-catenin to relay signal, have been demonstrated HSC function. In this study, we examined role noncanonical in regulating fate. We observed Wnt5a inhibited Wnt3a-mediated HSCs suppressed alterations gene expression associated with differentiation, such as...
Myelofibrosis is a severe myeloproliferative neoplasm characterized by increased numbers of abnormal bone marrow megakaryocytes that induce fibrosis, destroying the hematopoietic microenvironment. To determine cellular and molecular basis for aberrant megakaryopoiesis in myelofibrosis, we performed single-cell transcriptome profiling 135,929 CD34+ lineage− stem progenitor cells (HSPCs), proteomics, genomics, functional assays. We identified bias toward megakaryocyte differentiation apparent...
Abstract Mobile weather radars often utilize rapid-scan strategies when collecting observations of severe weather. Various techniques have been used to improve volume update times, including the use agile and multibeam radars. Imaging radars, similar in some respects phased arrays, steer radar beam software, thus requiring no physical motion. In contrast imaging gather data for an entire simultaneously within field view (FOV) radar, which is defined by a broad transmit beam. As result,...
Human hematopoiesis is a dynamic process that starts in utero 18–21 days post-conception. Understanding the site- and stage-specific variation important if we are to understand origin of hematological disorders, many which occur at specific points human lifespan. To unravel how hematopoietic stem/progenitor cell (HSPC) compartment changes during ontogeny underlying gene regulatory mechanisms, compare 57,489 HSPCs from 5 different tissues spanning 4 developmental stages through lifetime....
Abstract The scientific community has expressed interest in the potential of phased array radars (PARs) to observe atmosphere with finer spatial and temporal scales. Although convergence occurred between meteorological engineering communities, need exists increase access PAR meteorologists. Here, we facilitate these interdisciplinary efforts field ground-based PARs for atmospheric studies. We cover high-level technical concepts terminology as applied studies atmosphere. A historical...
Weather radars have become indispensable to meteorologists and the general public for both understanding awareness of high-impact weather events as part operational warning infrastructure. In U.S., radar network is composed approximately 160 WSR-88D radars, which are S-band, dish-based, polarimetric Doppler radars. This work reports on development a fully digital phased array that being used assess potential such technology replacement The “Horus” truck-based, radar. Fully systems hold...
Pluripotent hematopoietic stem cells (PHSCs) were highly enriched from mouse bone marrow by counterflow centrifugal elutriation, lineage subtraction, and fluorescence-activated cell sorting based on high c-kit receptor expression (c-kitBR). We used reverse transcriptase polymerase chain reaction to assay the c-kitBR subset subsets expressing low (c-kitDULL) no (c-kitNEG) for of mRNA encoding growth factor receptors transcription factors. The had approximately 3.5-fold more than...
Hmgb3 is an X-linked member of a family sequence-independent chromatin-binding proteins that preferentially expressed in hematopoietic stem cells (HSC). Hmgb3-deficient mice (Hmgb3(-/Y)) contain normal numbers HSCs, capable self-renewal and repopulation, but fewer common lymphoid (CLP) myeloid progenitors (CMP). In this study, we tested the hypothesis Hmgb3(-/Y) HSCs are biased toward at expense progenitor production. Wild-type CLPs CMPs proliferate differentiate equally vitro, indicating...
Erythroid Kru ¨ppel-like factor (EKLF) is a transcription identified as transcriptional activator and chromatin modifier in erythroid cells.EKLF-deficient (Eklf ؊/؊ ) mice die at day 14.5 of gestation from severe anemia.In this study, we demonstrate that early progenitor cells fail to undergo terminal differentiation Eklf embryos.To discover potential EKLF target genes responsible for the failure erythropoiesis, profiling was performed with RNA wild-type cells.These analyses significant...
Thousands of epigenomic data sets have been generated in the past decade, but it is difficult for researchers to effectively use all relevant their projects. Systematic integrative analysis can help meet this need, and VISION project was established
Genotype-phenotype correlations are revealed by bone marrow single-cell RNA sequencing in Diamond-Blackfan anemia.
A tornado outbreak occurred in central Oklahoma on 10 May 2010, including two tornadoes with enhanced Fujita scale ratings of 4 (EF-4). Tragically, three deaths were reported along significant property damage. Several strong and violent near Norman, Oklahoma, which is a major hub for severe storms research arguably one the best observed regions country multiple Doppler radars operated by both federal government University (OU). One most recent additions to Norman high-resolution OU...
Abstract This study investigates the use of tornadic debris signature (TDS) parameters to estimate tornado damage severity using Norman, Oklahoma (KOUN), polarimetric radar data (polarimetric version Weather Surveillance Radar-1988 Doppler radar). Several TDS are examined, including based on 10th or 90th percentiles variables (lowest tilt parameters) and volumetric coverage (spatial parameters). Two highly detailed National Service (NWS) surveys compared parameters. The tend be correlated...
Abstract Mobile radar platforms designed for observation of severe local storms have consistently pushed the boundaries spatial and temporal resolution in order to allow detailed analysis storm structure evolution. Digital beamforming, or imaging, is a technique that similar nature photograwphic camera, where data samples from different spaces at same range are collected simultaneously. This allows rapid volumetric update rates compared radars scan with single narrow beam. The Atmospheric...