A5026333854- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Pneumonia and Respiratory Infections
- Antibiotic Use and Resistance
- Genetic diversity and population structure
- Pancreatic function and diabetes
- CRISPR and Genetic Engineering
- Evolution and Genetic Dynamics
- Diabetes Management and Research
- Genomics and Chromatin Dynamics
- Diabetes and associated disorders
- Pluripotent Stem Cells Research
- Amphibian and Reptile Biology
- Respiratory viral infections research
- Human-Automation Interaction and Safety
- Molecular Biology Techniques and Applications
- Species Distribution and Climate Change
- Rough Sets and Fuzzy Logic
- Congenital heart defects research
- Text and Document Classification Technologies
- Color perception and design
- Neural and Behavioral Psychology Studies
- Traffic and Road Safety
- Wildlife Ecology and Conservation
- Innovative Human-Technology Interaction
Yunnan University
2014-2024
Kunming Institute of Zoology
2015-2024
Chinese Academy of Sciences
2015-2024
Hubei University of Arts and Science
2021-2024
Anhui Medical University
2012-2021
University of Chinese Academy of Sciences
2015-2020
Institute of Psychology, Chinese Academy of Sciences
2014-2020
Center for Life Sciences
2019
City of Hope
2014-2015
Beckman Research Institute
2014-2015
Abstract Common carp ( Cyprinus carpio ) is an allotetraploid species derived from recent whole genome duplication and provides a model to study polyploid evolution in vertebrates. Here, we generate three chromosome-level reference genomes of C. compare related diploid Cyprinid genomes. We identify Barbinae lineage as potential progenitor then divide the into two subgenomes marked by distinct similarity progenitor. estimate that progenitors diverged around 23 Mya merged 12.4 based on...
To meet the need of a large quantity hPSC-derived cardiomyocytes (CM) for pre-clinical and clinical studies, robust scalable differentiation system CM production is essential. With human pluripotent stem cells (hPSC) aggregate suspension culture we established previously, developed matrix-free, scalable, GMP-compliant process directing hPSC to in by modulating Wnt pathways with small molecules. By optimizing critical parameters including: cell size, molecule concentrations, induction timing,...
Significance Why is polyploidization rarer in animals than plants? This question remains unanswered due to the absence of a suitable system for studying instantaneous and crucial changes that immediately follow hybridization. RNA-seq analyses discover extensive chimeric genes immediate mutations orthologs both diploid tetraploid offspring goldfish (♀) × common carp (♂) hybrids. Overall, show paternal-biased expression, yet tetraploids maternal-biased expression. Some differentially expressed...
VennPainter is a program for depicting unique and shared sets of genes lists generating Venn diagrams, by using the Qt C++ framework. The software produces Classic Venn, Edwards' Nested diagrams allows eight in graph mode 31 data processing only. In comparison, previous programs produce allow maximum six sets. incorporates user-friendly features works Windows, Linux Mac OS. Its graphical interface does not require user to have programing skills. Users can modify diagram content up datasets...
A persistent enigma is the rarity of polyploidy in animals, compared to its prevalence plants. Although animal polyploids are thought experience deleterious genomic chaos during initial polyploidization and subsequent rediploidization processes, this hypothesis has not been tested. We provide an improved reference-quality de novo genome for allotetraploid goldfish whose origin dates ~15 million years ago. Comprehensive analyses identify changes subgenomic evolution from asymmetrical...
Identifying and protecting hotspots of endemism species richness is crucial for mitigating the global biodiversity crisis. However, our understanding spatial diversity patterns far from complete, which severely limits ability to conserve hotspots. Here, we report a comprehensive analysis amphibian in China, one most species-rich countries on Earth. Our study combines 20 y field surveys with new molecular analyses 521 described also identifies 100 potential cryptic species. We identify 10...
Effective conservation of threatened biota relies on accurate assessments and scientific guidance. As an unfortunate example, Chinese giant salamanders ( Andrias, CGS) remain critically endangered in nature. Misguided efforts, e.g., commercial propagation releasing millions likely non-indigenous or interspecific hybrids, have further compromised initiatives. Limited information wild populations CGS poses a significant challenge. Following 18-month long field monitoring, we now report the...
Progenitor cells in the adult pancreas are potential sources of endocrine beta for treating type 1 diabetes. Previously, we identified tri-potent progenitor (2-4month-old) murine that were capable self-renewal and differentiation into duct, acinar, vitro. These named pancreatic colony-forming units (PCFUs). However, because PCFUs a minor population (~1%) they difficult to study. To enrich PCFUs, strategies using cell-surface marker analyses fluorescence-activated cell sorting developed. We...
Abstract Hybridization and polyploidization are considered important driving forces that form new epigenetic regulations. To study the changing patterns of expression accompanying hybridization polyploidization, we used RNA-seq qRT-PCR to investigate global homoeologue in diploid tetraploid hybrids Carassius auratus red var. (♀) (R) Cyprinus carpio (♂) (C). By comparing relative levels between their parents, defined level dominance (ELD) bias (HEB) liver tissue. The results showed...
The behavioral and cognitive characteristics of dangerous drivers differ significantly from those safe drivers. However, differences in emotional information processing have seldom been investigated. Previous studies revealed that with higher anger/anxiety trait scores are more likely to be involved crashes individuals anger traits exhibit stronger negativity biases when emotions compared control groups. researchers not explored the relationship between driving behavior. In this study, we...
Previous studies describe a unique culture method for the commitment of murine embryonic stem cells to early endocrine pancreata. In this report, pancreatic-like β-cell progenitors were enriched and colony assay devised allow these differentiate into insulin-expressing colonies in vitro. An cell line with enhanced green fluorescent protein (EGFP) inserted one allele neurogenin 3 (Ngn3), marker pancreatic progenitors, was differentiated. During late stage culture, 20–30% Ngn3-EGFP+. Gene...
Embryonic stem (ES) cell technology may serve as a platform for the discovery of drugs to treat diseases such diabetes. However, because difficulties in establishing reliable ES differentiation methods and creating cost-effective plating conditions high-throughput format, screening molecules that regulate pancreatic beta cells their immediate progenitors has been limited. A relatively simple inexpensive protocol allows efficient generation insulin-expressing from murine was previously...
Aims. This article aims at analyzing regional differences in smoking, drinking, and physical activities China informing cross-regional initiatives addressing these behaviors. Methods. Data were extracted from available large-scale surveys. Index of dissimilarity (ID), linear correlation, geographical mapping used to analyze the prevalence smoking (PS), cessation (PSC), regular drinking (PRD), (PPA). Results. Significant existed across regions all behaviors ( P < .01). IDs ranged 0.04...
A new Usability Metric for User Experience (UMUX) is translated and validated native Chinese speakers in this study. The forward-backward translation method applied to translate the UMUX. results are optimized through structure-back interviews obtain a final, unambiguous UMUX version. version of questionnaire proven have high reliability, sensitivity, effectiveness. correspondence between version, UMUX-LITE, SUS also investigated. work may provide usability practitioners with standardized...
The phylogenetic relationships of Asian schilbid catfishes the genera Clupisoma, Ailia, Horabagrus, Laides and Pseudeutropius are poorly understood, especially those Clupisoma. Herein, we reconstruct phylogeny 38 species belonging to 28 14 families using concatenated mitochondrial genes COI, cytb, 16S rRNA, as well nuclear RAG1 RAG2. resulting trees consistently place Clupisoma sister taxon Laides, five representative form two monophyletic groups with (Ailia (Laides, Clupisoma)) (Horabagrus,...
Prader-Willi and Angelman syndrome (PWS AS) patients typically have an approximately 5 Mb deletion of human chromosome 15q11-q13, opposite parental origin. A mouse model PWS AS has a transgenic insertion-deletion (TgPWS/TgAS) 7B/C subsequent to paternal or maternal inheritance, respectively. In this study, we define the endpoints examine impact on expression flanking genes.Using molecular cytological methods demonstrate that 13 imprinted 11 non-imprinted genes are included in TgPWS/TgAS...
The Review of Diabetic Studies,2014,11,1,35-50.DOI:10.1900/RDS.2014.11.35Published:May 2014Type:Original ArticleAuthors:Liang Jin, Tao Feng, Jing Chai, Nadiah Ghazalli, Dan Gao, Ricardo Zerda, Zhuo Li, Jasper Hsu, Alborz Mahdavi, David A. Tirrell, Arthur D. Riggs, and Hsun Teresa Ku Author(s) affiliations:Liang Jin1,*, Feng1,*, Chai1,*, Ghazalli1,2, Gao1, Zerda3, Li3, Hsu1, Mahdavi4, Tirrell5, Riggs1, Ku1,2 1Department Diabetes Metabolic Diseases Research, Beckman Research Institute, City...