A5057089508- Sulfur Compounds in Biology
- Redox biology and oxidative stress
- Sulfur-Based Synthesis Techniques
- Polyamine Metabolism and Applications
- Synthesis and Catalytic Reactions
- Chemical Synthesis and Analysis
- Neuroscience of respiration and sleep
- Chemical Synthesis and Reactions
- Plant Pathogens and Fungal Diseases
- Chemical Reaction Mechanisms
- Eicosanoids and Hypertension Pharmacology
- Renin-Angiotensin System Studies
- Plant-Microbe Interactions and Immunity
- Mast cells and histamine
- RNA and protein synthesis mechanisms
- Adenosine and Purinergic Signaling
- Plant Pathogens and Resistance
- Organic and Inorganic Chemical Reactions
- Organophosphorus compounds synthesis
- Mycorrhizal Fungi and Plant Interactions
- Phosphorus compounds and reactions
- Atmospheric chemistry and aerosols
- Industrial Gas Emission Control
- Click Chemistry and Applications
- DNA and Nucleic Acid Chemistry
Gangneung–Wonju National University
2016-2024
Washington State University
2011-2022
Seattle University
2016
Division of Chemistry
2015
Duke University Hospital
2012
Duke Medical Center
2012
Abstract Protein S‐sulfhydration (forming ‐S‐SH adducts from cysteine residues) is a newly defined oxidative posttranslational modification and plays an important role in H 2 S‐mediated signaling pathways. In this study we report the first selective, “tag‐switch” method which can directly label protein S‐sulfhydrated residues by forming stable thioether conjugates. Furthermore demonstrate that S alone cannot lead to two possible physiological mechanisms include reaction with sulfenic acids...
Hydrogen sulfide (H2S) is a critical signaling molecule that regulates many physiological and/or pathological processes. Modulation of H2S levels could have potential therapeutic value. In this work, we report the rational design, synthesis, and biological evaluation class phosphonamidothioate-based H2S-releasing agents (i.e., donors). A novel pH-dependent intramolecular cyclization was employed to promote release from donors. These water-soluble compounds showed slow, controllable,...
A reaction based fluorescence turn-on strategy for hydrogen sulfide (H2S) was developed. This on a H2S-specific Michael addition–cyclization sequence. Other biological thiols such as cysteine and glutathione did not pursue the therefore turn fluorescence/consume substrates. The probes showed good selectivity sensitivity sulfide.
"Caged" gem-dithiol derivatives that release H2S upon light stimulation have been developed. This new class of donors was proven, by various spectroscopic methods, to generate in an aqueous/organic medium as well cell culture.
The development of a functional disulfide, FmSSPy-A (Fm = 9-fluorenylmethyl; Py pyridinyl), is reported. It can effectively convert small molecule and protein thiols (−SH) to form −S-SFm adducts under mild conditions. This method allows for H2S-free biomimetic protocol generate highly reactive persulfides (in their anionic forms). high nucleophilicity toward number thiol-blocking reagents also demonstrated. holds promise further understanding the chemical biology S-sulfhydration.
A series of O-aryl- and alkyl-substituted phosphorodithioates were designed synthesized as hydrogen sulfide (H2S) donors. H2S releasing capability these compounds was evaluated using fluorescence methods. O-aryl substituted donors showed slow sustained release while O-alkylated very weak capability. We also donors' protective effects against peroxide (H2O2)-induced oxidative damage in myocytes toxicity toward B16BL6 mouse melanoma cells.
A class of novel thiol-activated H2S donors has been developed on the basis gem-dithiol template. These release in presence cysteine or GSH aqueous solutions as well cellular environments.
Background Hydrogen sulfide (H 2 S) is a gasotransmitter that regulates multiple cardiovascular functions. Krüppel‐like factor 5 (KLF5) exerts diverse functions in the system. Whether and how H S KLF5 myocardial hypertrophy unknown. Methods Results In our study, hypertrophic samples clinic were collected underwent histological molecular biological analysis. Spontaneously hypertensive rats neonatal rat cardiomyocytes studied for functional signaling responses to GYY4137, an S‐releasing...
A synthesis of aziridine-containing peptides via the Cu(II)-promoted coupling unprotected peptide thioacids and N-H aziridine-2-carbonyl is reported. The unique reactivity resulting N-acylated facilitates their subsequent regioselective stereoselective nucleophilic ring-opening to give that are specifically modified at ligation site. aziridine-mediated concept exemplified using H(2)O as nucleophile, producing a Xaa-Thr linkage (where Xaa can be an epimerizable hindered amino acid). overall...
The selective reaction of chemical reagents with reduced protein thiols is critical to biological research. This utilized prevent cross-linking cysteine-containing peptides in common proteomics workflows and applied widely discovery targeted redox investigations the mechanisms underlying physiological pathological processes. However, known commonly used thiol blocking like iodoacetamide, N-ethylmaleimide, others were found cross-react oxidized sulfenic acids (−SOH) introducing significant...
Abstract Chemotaxonomy and the comparative analysis of metabolic features fungi have potential to provide valuable information relating ecology evolution, but not been fully explored in fungal biology. Here, we investigated chemical diversity legume-associated Ascochyta Phoma species possible use a metabolomics approach using liquid chromatography-mass spectrometry for their classification. The 45 strains including 11 known isolated from various legumes were extracted datasets analyzed...
Ascochyta rabiei and Alternaria solani, the causal agents of blight chickpea (Cicer arietinum) early potato (Solanum tuberosum), respectively, produce a set phytotoxic compounds including solanapyrones A, B, C. Although both phytotoxicity their universal production among field isolates have been documented, role in pathogenicity is not well understood. Here, we report functional characterization sol5 gene, which encodes Diels-Alderase that catalyzes final step solanapyrone biosynthesis....
Ascochyta rabiei and Alternaria solani, the causal agents of blight chickpea (Cicer arietinum) early potato (Solanum tuberosum), respectively, produce a set phytotoxic compounds including solanapyrones A, B, C. Although both phytotoxicity their universal production among field isolates have been documented, role in pathogenicity is not well understood. Here, we report functional characterization sol5 gene, which encodes Diels-Alderase that catalyzes final step solanapyrone biosynthesis....
Abstract Protein‐Persulfidbildung (die Bildung von ‐S‐SH‐Addukten aus Cysteinresten) ist eine vor kurzem entdeckte oxidative posttranslationale Modifikation, die wichtige Rolle bei H 2 S‐vermittelten Signalwegen spielt. Hier berichten wir über erste selektive “Tag‐Switch”‐Methode, mit der Protein‐Persulfidreste direkt markiert werden können, um stabile Thioether‐Konjugate zu bilden. Des Weiteren zeigen wir, dass S allein nicht zur Persulfidbildung führen kann und beiden möglichen...
1,3,5-Trithiane functionalized with esterase-sensitive ester groups on the methylene linkers was developed as a class of enzymatic hydrolysis-based hydrogen sulfide (H<sub>2</sub>S) donors.
The reactions between S-nitrosothiols and phosphite esters, including P(OPh)3, P(OBn)3, P(OEt)3, were studied. Two different conjugated adducts, thiophosphoramidates phosphorothioates, formed, depending on the structures of S-nitrosothiol substrate (e.g., primary vs tertiary). These proceeded under mild conditions, reaction mechanisms studied using experiments calculations.
This study presents a global strategy for the transsulfuration of intracellular thiols (RSH) to persulfides (RSSH). Thiiranes comprising fluorenyl/diphenyl and malonate ester moieties directly convert intercellular RSH low-molecular-weight RSSH in cells. The efficiency is determined by counting number olefins produced as byproducts, providing ratiometric signals corresponding persulfide production. Specifically, direct rapid protein S-persulfidation thiirane validated. are expected play...